This list is intended to include all significant botanical gardens and arboretums in the U.S. state of Florida.
| Name | Image | Affiliation | City |
|---|
| American Orchid Society Visitor Center and Botanical Garden | American Orchid Society | Delray Beach |
| Arboretum of the University of Central Florida | University of Central Florida | Orlando |
| Bok Tower Gardens | Lake Wales |
| Botanic Gardens at Kona Kai Resort | Key Largo |
| Cypress Gardens | Winter Haven |
| Cummer Museum of Art and Gardens | Jacksonville |
| Deerfield Beach Arboretum | Deerfield Beach |
| Edison and Ford Winter Estates | Fort Myers |
| Fairchild Tropical Botanic Garden | Coral Gables |
| Flamingo Gardens | Davie |
| The Florida Botanical Gardens | Largo |
| Florida Institute of Technology Botanical Garden | Florida Institute of Technology | Melbourne |
| Four Arts Gardens | Palm Beach |
| Fruit and Spice Park | Homestead |
| John C. Gifford Arboretum | University of Miami | Coral Gables |
| Heathcote Botanical Gardens | Fort Pierce |
| Jacksonville Arboretum & Gardens | Jacksonville |
| Jacksonville Zoo and Gardens | Jacksonville |
| The Kampong | National Tropical Botanical Garden | Miami |
| Kanapaha Botanical Gardens | Gainesville |
| Key West Botanical Forest and Garden | Key West |
| Harry P. Leu Gardens | Orlando |
| Alfred B. Maclay State Gardens | Tallahassee |
| McKee Botanical Garden | Vero Beach |
| Miami Beach Botanical Garden | Miami Beach |
| Montgomery Botanical Center | Coral Gables |
| Morikami Museum and Japanese Gardens | Delray Beach |
| Mounts Botanical Garden | West Palm Beach |
| Naples Botanical Garden | Naples |
| Nature Coast Botanical Gardens | Spring Hill |
| Ormond Memorial Art Museum and Gardens | Ormond Beach |
| Palm and Cycad Arboretum | Florida Community College | Jacksonville |
| Palma Sola Botanical Park | Bradenton |
| Roji-en Japanese Gardens | Morikami Museum and Japanese Gardens | Delray Beach |
| Sarasota Jungle Gardens | Sarasota |
| Marie Selby Botanical Gardens | Sarasota |
| Sunken Gardens | St. Petersburg |
| Tree Hill Nature Center | Jacksonville |
| Tropical Ranch Botanical Garden | Stuart |
| Unbelievable Acres Botanic Gardens | Palm Beach |
| University of South Florida Botanical Gardens | University of South Florida | Tampa |
| Vizcaya Museum and Gardens | Miami |