This list is intended to include all significant botanical gardens and arboretums in the U.S. state of Pennsylvania.
| Name | Image | Affiliation | City |
|---|
| Ambler Arboretum | Temple University | Ambler |
| American College Arboretum | The American College | Bryn Mawr |
| Appleford/Parsons-Banks Arboretum | Villanova |
| Arboretum at Penn State | Pennsylvania State University | State College |
| Arboretum at the Reading Public Museum | Reading Public Museum | Reading |
| Arboretum at Penn State Behrend | Penn State Erie, The Behrend College | Erie |
| Arboretum of the Barnes Foundation | Barnes Foundation | Merion |
| Arboretum Villanova | Villanova University | Villanova |
| Awbury Arboretum | Philadelphia (East Germantown) |
| Bartram's Garden | Philadelphia (Fairmount Park) |
| Bowman's Hill Wildflower Preserve | New Hope |
| Brandywine Wildflower and Native Plant Gardens | Brandywine River Museum | Chadds Ford |
| Bryn Mawr Campus Arboretum | Bryn Mawr College | Bryn Mawr |
| Centennial Arboretum | Philadelphia (Fairmount Park) |
| Chanticleer Garden | Wayne |
| Chatham University Arboretum | Chatham University | Pittsburgh |
| Crozer Arboretum | Upland |
| William F. Curtis Arboretum | Cedar Crest College | Allentown |
| Curtis Hall Arboretum | Wyncote |
| Lee and Virginia Graver Arboretum | Muhlenberg College | Bath |
| Haverford College Arboretum | Haverford College | Haverford |
| Henry Foundation for Botanical Research | Gladwyne |
| Hershey Gardens | M.S. Hershey Foundation | Hershey |
| Holtwood Arboretum | Holtwood |
| The Horticulture Center | Philadelphia (Fairmount Park) |
| Jenkins Arboretum | Devon |
| Lake Erie Arboretum | Erie |
| Liberty Forge Arboretum | Mechanicsburg |
| Longwood Gardens | Kennett Square |
| Marywood University Arboretum | Marywood University | Scranton |
| Merion Botanical Park | Merion |
| Mont Alto Arboretum | Penn State Mont Alto | Mont Alto |
| Morris Arboretum | University of Pennsylvania | Philadelphia |
| National Aviary | Pittsburgh |
| Phipps Conservatory & Botanical Gardens | Pittsburgh |
| Pittsburgh Botanic Garden | Pittsburgh |
| Renziehausen Park Rose Garden and Arboretum | McKeesport |
| Rodef Shalom Biblical Botanical Garden | Rodef Shalom Congregation | Pittsburgh |
| Henry Schmieder Arboretum | Delaware Valley College | Doylestown |
| Scott Arboretum | Swarthmore College | Swarthmore |
| Swiss Pines | Malvern |
| Louise Arnold Tanger Arboretum | Lancaster |
| Taylor Memorial Arboretum | Wallingford |
| John J. Tyler Arboretum | Media |
| Welkinweir | Pottstown |