| Name | Source | Type | | Eber | 2300 AD |
| Ectonurite | Ben 10 | Aliens from planet Anur Phateos, their abilities around those of ghosts. They can become invisible or intangible, fly, unleash tentacles from within their bodies, and possess enemies. However, they wear protective skin that limits their powers, as they burn when exposed to sunlight. |
| Edestekai | Star Frontiers | trilaterally symmetrical nomads |
| Edo | Star Trek |
| Ego the Living Planet | Marvel Comics |
| Ekhonides | Perry Rhodan |
| El-Aurians | Star Trek | Humanoid race of "listeners" |
| Eldar | Warhammer 40,000 | Humanoid |
| Elder Gods | Cthulhu Mythos |
| Elder Thing | Cthulhu Mythos |
| Eldorians | Utopia |
| Electrogoomba | Super Mario Galaxy | Evolved species of a Goomba, the species is divided into different variants |
| Elerians | Master of Orion II | Humanoid |
| Elites (Sangheili) | Halo | Technologically superior race of military supremacy. Mainstay of Covenant military until philosophical differences removed them from the Hierarchy. They allied themselves to Humans to combat Covenant. Uncertain allies to humans. |
| Ellimist | K. A. Applegate's Animorphs | All powerful being with no known appearance |
| Elves | Bravest Warriors | These creatures are nearly all virtual except for Wankershim, who exited the Holo John and became a separate life form. Wankershim, however, then enveloped the whole universe. |
| Elvis | Perfect Dark | Grey |
| Elygem | Pokémon |
| Engineers (Huragok) | Halo | Maintain Halo Rings, not officially part of covenant. Large, pink, squid like creatures. No real threat to Humanity other than maintaining the Halo Rings, which ARE a threat to humanity. |
| Engineers | Prometheus (2012 film) | 9 ft tall pale white muscular humanoids, the supposed genetic originators of humankind. |
| Eoladi | Master of Orion III |
| Eorna | Star Frontiers | Highly advanced reptilians facing extinction |
| Eosapien | Alien Planet |
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| Ep-Hogers | Perry Rhodan |
| Eppori | Harry Turtledove's Worldwar series | An animal native to Home. Its appearance was described resembling "a cross between a zebra and a duckbilled dinosaur" striped in a pattern of gold and dark brown. It was domesticated by the Race early in its history and was used as a riding animal and beast of burden. With the advent of mechanized vehicles, the eppori in Race society was mostly rendered obsolete. However, they continued to be used by zisuili farmers and others for attraction. |
| Erg | Hyperion Cantos | Silicon-based life forms |
| Eridani | Battlelords of the 23rd Century | Humanoid |
| Eschiff | London Wild | Feline Humanoid |
| Esmers | Little Big Adventure 2 |
| Esperians | Star Fleet | Humanoid |
| E.T | E.T. the Extra-Terrestrial |
| Ethereals | UFO Defense | Alien with telepathic powers capable of breaking the laws of physics and manipulating other beings (such as humans) to do their bidding. Even through their bodies should not be able to keep them alive as they have no functioning organs (other than their brain) they can still live. |
| Exobot | Star Trek |
| Extraterrestrial Biological Entities (EBE) | The Event | Aliens that strongly resemble humans by 99%. They have enhanced lifespans, meaning they age at a slow rate. |
| Exquivan | Star Control 3 |
| Extraterrestrial Living-metal Shape-shifter (ELS) | Awakening of the Trailblazer |
| Evon | Master of Orion III | Humanoid |
| Evroniani | PKNA - Paperinik New Adventure | Ducks-like humanoid |
| Ewoks | Star Wars | Small furry humanoids |
| Eywa | James Cameron's Avatar | Deity / Neural connection between trees |