Mark 27 may refer to:

• Mark 27 nuclear bomb, 1950s United States nuclear weapon design
• Mark 27 torpedo, 1940s United States Navy submarine-launched torpedo
• Mark 27 mine, submarine-launched mobile ground mine

• Roslindale, Boston, Massachusetts
• Roslindale Congregational Church
• Roslindale Village station
• Roslin (disambiguation)
• Roslin House
• Roslin, Ontario
• Rosliston Primary School
• Rosli Streiff
• Roslonek
• Roslonki
• Roslund/Hellström
• Roslyakovo, Murmansk Oblast
• Roslyn AFS
• Roslyn Air National Guard Station
• Roslyn Atkinson
• Roslyn Bridge
• Roslyn Brock
• Roslyn Brogue
• Roslyn (disambiguation)
• Roslyn, Dunedin
• Roslyn Flats
• Roslyn Grist Mill
• Roslyn Harbor, NY
• Roslyn Heights, NY
• Roslyn high school