| 词条 | Pithovirus |
| 释义 |
|image = Pithovirus sibericum sketch.jpg |virus_group = i |ordo = Megavirales |familia = Pithoviridae |genus = Pithovirus |subdivision_ranks = Species |subdivision =
}} Pithovirus, first described in a 2014 paper, is a genus of giant virus known from one species, Pithovirus sibericum, which infects amoebas.[1][2] It is a double-stranded DNA virus, and is a member of the nucleocytoplasmic large DNA viruses clade. The 2014 discovery was made when a viable specimen was found in a 30,000-year-old ice core harvested from permafrost in Siberia, Russia. DescriptionThe genus name Pithovirus, a reference to large storage containers of ancient Greece known as pithoi, was chosen to describe the new species. A specimen of Pithovirus measures approximately 1.5 µm (1500 nm) in length and 0.5 µm (500 nm) in diameter, making it the largest virus yet found. It is 50% larger in size than the Pandoraviridae, the previous largest known viruses,[3] is larger than Ostreococcus the smallest eukaryotic cell, although Pandoravirus has the largest viral genome, containing 1.9 to 2.5 megabases of DNA.[4] Pithovirus has a thick, oval wall with an opening at one end. Internally, its structure resembles a honeycomb.[1]The genome of Pithovirus contains 467 distinct genes, more than a typical virus but far fewer than the 2556 putative protein-coding sequences found in Pandoravirus.[3] Thus, its genome is far less densely packed than any other known virus. Two-thirds of its proteins are unlike those of other viruses. Despite the physical similarity with Pandoravirus, the Pithovirus genome sequence reveals that it is barely related to that virus, but more closely resembles members of Marseilleviridae, Megaviridae, and Iridoviridae.[7] These families all contain large icosahedral viruses with DNA genomes. The Pithovirus genome has 36% GC-content, similar to the Megaviridae, in contrast to greater than 61% for pandoraviruses.[8] ReplicationPithovirus' genome is one circular, double-stranded DNA (dsDNA) chromosome of about 610,000 base pairs (bp), encoding approximately 467 open reading frames (ORFs), which translate into 467 different proteins.[5] The genome encodes all the proteins needed to produce mRNA; these proteins are present in the purified virions.[7] Pithovirus therefore undergoes its entire replication cycle in its host's cytoplasm, rather than the more typical method of taking over the host's nucleus.[1][7][6]DiscoveryPithovirus sibericum was discovered in a 30,000-year-old sample of Siberian permafrost by Chantal Abergel and Jean-Michel Claverie of Aix-Marseille University.[1][7] The virus was discovered buried {{convert|30|m|ft|abbr=on|sigfig=1}} below the surface of a late Pleistocene sediment.[2][8] It was found when riverbank samples collected in 2000 were exposed to amoebas.[9] The amoebas started dying and when examined were found to contain giant virus specimens. The authors said they got the idea to probe permafrost samples for new viruses after reading about an experiment that revived a similar aged seed of Silene stenophylla two years earlier.[1] The Pithovirus findings were published in the Proceedings of the National Academy of Sciences in March 2014.[3][10]Although the virus is harmless to humans, its viability after being frozen for millennia has raised concerns that global climate change and tundra drilling operations could lead to previously undiscovered and potentially pathogenic viruses being unearthed.[3] However, other scientists dispute that this scenario poses a real threat.[1] EvolutionThe rate of mutation of the genome has been estimated to be 2.23 × 10−6 subsitutions/site/year.[11] The authors have suggested that these viruses evolved at least hundreds of thousands of years ago. Other viruses in this genusA second virus in this genus, Pithovirus massiliensis, has been isolated.[12] Comparison of the genes between the two species suggests a mutation rate of 3 x 10−6 per life. See also{{Portal|Viruses}}
References1. ^1 2 3 4 5 {{cite journal |last=Yong |first=Ed |doi=10.1038/nature.2014.14801 |title=Giant virus resurrected from 30,000-year-old ice |journal=Nature |date=3 March 2014}} 2. ^1 {{cite news |first=Rebecca |last=Morelle |title=30,000-year-old giant virus 'comes back to life' |date=3 March 2014 |url=https://www.bbc.com/news/science-environment-26387276 |work=BBC News}} 3. ^1 2 3 {{cite news |title=Ancient "Giant Virus" Revived From Siberian Permafrost |work=National Geographic |date=3 March 2014 |last=Sirucek |first=Stefan |url=http://news.nationalgeographic.com/news/2014/03/140303-giant-virus-permafrost-siberia-pithovirus-pandoravirus-science/}} 4. ^{{cite news |url=https://www.npr.org/blogs/health/2013/07/18/203298244/worlds-biggest-virus-may-have-ancient-roots |title=World's Biggest Virus May Have Ancient Roots |work=National Public Radio |date=18 July 2013 |last=Brumfiel |first=Geoff}} 5. ^{{cite web |url= http://viralzone.expasy.org/all_by_species/4237.html |title= Pithovirus sibericum |accessdate= 2014-03-10 |work= Swiss Institute of Bioinformatics (SIB)}} 6. ^{{cite news |first=Andy |last=Coghlan |title=Biggest-ever virus revived from Stone Age permafrost |date=3 March 2014 |url=https://www.newscientist.com/article/dn25151-biggestever-virus-revived-from-stone-age-permafrost.html |work=NewScientist |archivedate=2014-03-05 |archiveurl=https://web.archive.org/web/20140305155750/http://www.newscientist.com/article/dn25151-biggestever-virus-revived-from-stone-age-permafrost.html |deadurl=no}} 7. ^{{cite news |last=Pappas |first=Stephanie |url= https://news.yahoo.com/frozen-giant-virus-still-infectious-30-000-years-173243936.html |title=Frozen Giant Virus Still Infectious After 30,000 Years |work=Yahoo News |date=16 September 2015 |accessdate=2015-09-20}} 8. ^1 2 3 {{cite news |first=Vincent |last=Racaniello |title=Pithovirus: Bigger than Pandoravirus with a smaller genome |date=4 March 2014 |url=http://www.virology.ws/2014/03/04/pithovirus-bigger-than-pandoravirus-with-a-smaller-genome/ |work=Virology Blog}} 9. ^{{cite news |title=Out of Siberian Ice, a Virus Revived |work=The New York Times |date=3 March 2014 |first=Carl |last=Zimmer |url=https://www.nytimes.com/2014/03/04/science/out-of-siberian-ice-a-virus-revived.html}} 10. ^1 {{cite journal |doi= 10.1073/pnas.1320670111 |title= Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology |journal= Proc. Natl. Acad. Sci. U.S.A. |volume= 111 |issue= 11 |pages= 4274–9 |date= March 2014 |pmid= 24591590 |last1= Legendre |first1= M. |last2= Bartoli |first2= J. |last3= Shmakova |first3= L. |last4= Jeudy |first4= S. |last5= Labadie |first5= K. |last6= Adrait |first6= A. |last7= Lescot |first7= M. |last8= Poirot |first8= O. |last9= Bertaux |first9= L. |last10= Bruley |first10= C. |last11= Coute |first11= Y. |last12= Rivkina |first12= E. |last13= Abergel |first13= C. |last14= Claverie |first14= J.-M.|pmc= 3964051 }} 11. ^Duchêne S, Holmes EC (2018) Estimating evolutionary rates in giant viruses using ancient genomes. Virus Evol 4(1):vey006. doi: 10.1093/ve/vey006 12. ^{{cite journal |vauthors=Levasseur A, Andreani J, Delerce J, Bou Khalil J, Robert C, La Scola B, Raoult D |title=Comparison of a modern and fossil Pithovirus reveals its genetic conservation and evolution |journal=Genome Biol Evol |volume= 8|issue= |pages= 2333–9|date=July 2016 |pmid=27389688 |doi=10.1093/gbe/evw153 |pmc=5010891}} External links
3 : Nucleocytoplasmic large DNA viruses|Discovered cryopreserved organisms|Species described in 2014 |
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