| 词条 | Sulforhodamine B |
| 释义 |
| verifiedrevid = 470482790 | Name = Sulforhodamine B | ImageFile = SulforhodamineB.png | ImageName = Sulforhodamine B | IUPACName = 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate | Section1 = {{Chembox Identifiers | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} | ChemSpiderID = 58690 | StdInChI_Ref = {{stdinchicite|correct|chemspider}} | StdInChI = 1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35) | StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} | StdInChIKey = IOOMXAQUNPWDLL-UHFFFAOYSA-N | CASNo_Ref = {{cascite|correct|CAS}} | CASNo = 3520-42-1 | PubChem = 65191 | EINECS = 220-025-2 | SMILES = O=S(=O)(O)c4ccc(C=1c3c(OC=2C=1\\C=C/C(=[N+](/CC)CC)/C=2)cc(cc3)N(CC)CC)c(c4)S([O-])(=O)=O | InChI = 1/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35) | InChIKey = IOOMXAQUNPWDLL-UHFFFAOYAJ | Section2 = {{Chembox Properties | Formula = C27H30N2O7S2 | MolarMass = 558.666 g/mol }}Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.[1] SpectroscopyThe dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.[1] It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.[2] ApplicationsSulforhodamine B is often used as a membrane-impermeable polar tracer[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).[4] References1. ^1 {{cite web |title=Sulforhodamine B sodium salt (CAS 3520-42-1) |publisher=Santa Cruz Biotechnology |url=http://www.scbt.com/fr/datasheet-253615-sulforhodamineb-sodium-salt.html |accessdate=}} 2. ^{{cite journal |last1=Coppeta |first1=J. |last2=Rogers |first2=C. |title=Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements |journal=Experiments in Fluids |date=1998 |volume=25 |pages=1–15 |doi=10.1007/s003480050202 }} 3. ^{{cite journal |author1=Viricel W |author2=Mbarek A |author3=Leblond J |title=Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery |journal=Angewandte Chemie International Edition |date=2015 |volume=54 |pages=12743–12747 |doi=10.1002/anie.201504661 |pmid=26189870}} 4. ^{{cite journal |author1=Vichai V |author2=Kirtikara K |title=Sulforhodamine B colorimetric assay for cytotoxicity screening |journal=Nature Protocols |date=2006 |volume=1 |pages=1112–1116 |doi=10.1038/nprot.2006.179}} 3 : Benzenesulfonates|Rhodamine dyes|Xanthenes |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。