| 词条 | Read (biology) |
| 释义 |
In DNA sequencing, a read is an inferred sequence of base pairs (or base pair probabilities) corresponding to all or part of a single DNA fragment. A typical sequencing experiment involves fragmentation of the genome into millions of molecules, which are size-selected and ligated to adapters. The set of fragments is referred to as a sequencing library, which is sequenced to produce a set of reads.[1] Read lengthSequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short.[2] Typical sequencers produce read lengths in the range of 100-500 bp.[3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp.[4] Read length is a factor which can affect the results of biological studies.[5] For example, longer read lengths improve the resolution of de novo genome assembly and detection of structural variants. It is estimated that read lengths greater than 100 kilobases (kb) will be required for routine de novo human genome assembly.[6] Bioinformatic pipelines to analyze sequencing data usually take into account read lengths.[7] References1. ^{{cite web|title=Sequencing library: what is it?|url=https://bredagenetics.com/sequencing-library-cosa-e/|website=Breda Genetics|accessdate=23 July 2017|date=2016-08-12}} 2. ^{{cite journal|last1=Chaisson|first1=Mark J.|title=De novo fragment assembly with short mate-paired reads: Does the read length matter?|journal=Genome Research|date=2009|volume=19|issue=2|pages=336–346|doi=10.1101/gr.079053.108|pmid=19056694|pmc=2652199|url=http://genome.cshlp.org/content/19/2/336.full|accessdate=23 July 2017}} 3. ^{{cite journal|last1=Junemann|first1=Sebastian|title=Updating benchtop sequencing performance comparison|journal=Nature Biotechnology|date=2013|volume=31|issue=4|pages=294–296|doi=10.1038/nbt.2522|pmid=23563421|url=https://www.nature.com/nbt/journal/v31/n4/full/nbt.2522.html|accessdate=23 July 2017}} 4. ^{{cite journal|last1=Quail|first1=Michael A.|title=A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers|journal=BMC Genomics|date=2012|volume=13|issue=1|page=341|doi=10.1186/1471-2164-13-341|pmid=22827831|pmc=3431227}} 5. ^{{cite journal|last1=Chhangawala|first1=Sagar|last2=Rudy|first2=Gabe|last3=Mason|first3=Christopher E.|last4=Rosenfeld|first4=Jeffrey A.|title=The impact of read length on quantification of differentially expressed genes and splice junction detection|journal=Genome Biology|date=23 June 2015|volume=16|issue=1|doi=10.1186/s13059-015-0697-y|pmid=26100517}} 6. ^{{cite journal|last1=Chaisson|first1=Mark J.P.|title=Genetic variation and the de novo assembly of human genomes|journal=Nature Reviews Genetics|date=2015|volume=16|issue=11|pages=627–640|doi=10.1038/nrg3933|pmid=26442640|url=http://www.nature.com/nrg/journal/v16/n11/full/nrg3933.html|accessdate=23 July 2017|pmc=4745987}} 7. ^{{cite journal|last1=Conesa|first1=Ana|last2=Madrigal|first2=Pedro|last3=Tarazona|first3=Sonia|last4=Gomez-Cabrero|first4=David|last5=Cervera|first5=Alejandra|last6=McPherson|first6=Andrew|last7=Szcześniak|first7=Michał Wojciech|last8=Gaffney|first8=Daniel J.|last9=Elo|first9=Laura L.|last10=Zhang|first10=Xuegong|last11=Mortazavi|first11=Ali|title=A survey of best practices for RNA-seq data analysis|journal=Genome Biology|date=26 January 2016|volume=17|issue=1|pages=13|doi=10.1186/s13059-016-0881-8|pmid=26813401|pmc=4728800}} 1 : DNA |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。