Tamblyn is a surname. Notable people with the surname include:

• Amber Tamblyn (born 1983), American actress and poet
• Christine Tamblyn, American artist
• Doni Tamblyn, author and comedian
• Eddie Tamblyn (1908–1957), American actor
• Geoff Tamblyn (born 1949), Australian cricketer
• Gordon Tamblyn, founder of the Tamblyn Drugs chain in Canada
• Harold Tamblyn-Watts (1900–1999), British artist
• Ian Tamblyn (born 1947), Canadian folk music singer-songwriter
• Russ Tamblyn (born 1934), actor

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