| 词条 | TE buffer |
| 释义 |
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. RecipeA typical recipe for making 1X TE buffer is:
TE buffer is also called as T10E1 Buffer, and read as "T ten E one buffer". To make a 100 ml solution of T10E1 Buffer, 1 ml of 1 M Tris base (pH 10-11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8. Based on nuclease studies from the 1980s, the pH is usually adjusted to 7.5 for RNA and 8.0 for DNA.{{Citation needed|date=September 2011}} The respective DNA and RNA nucleases are supposed to be less active at these pH values, but pH 8.0 can safely be used for storage of both DNA and RNA {{Citation needed|date=December 2012}}. EDTA further inactivates DNase, by binding to metal cations required by this enzyme.[1]Genomic and plasmid DNA can be stored in TE Buffer at 4 °C (39.2 °F) for short-term use, or -20 °C (-4 °F) to -80 °C (-112 °F) for long-term storage. Repeated freeze-thaw cycles should be avoided.[2] See also
Referencesph7.4 TE buffer=100mM/L Tris(pH7.4)+10mM/L EDTA(pH8.0) from Molecular Cloning: A Laboratory Manual 1. ^{{cite journal|vauthors=Yagi N, Satonaka K, Horio M, Shimogaki H, Tokuda Y, Maeda S |title=The role of DNase and EDTA on DNA degradation in formaldehyde fixed tissues|journal=Biotechnic & Histochemistry|year=1996|volume=71|issue=3|pages=123–129|pmid=8724437}} 2. ^{{cite journal|last=Ross|author2=Haites, Kelly |title=Repeated freezing and thawing of peripheral blood and DNA in suspension: effects on DNA yield and integrity|journal=Journal of Medical Genetics|year=1990|volume=27|issue=9|pages=569–570|url=http://jmg.bmj.com/content/27/9/569.short|doi=10.1136/jmg.27.9.569|pmc=1017219}} External links
1 : Buffers |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。