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词条 Stephen Bustin
释义

  1. Education

  2. Career

  3. Research

  4. Testimony

     Autism omnibus trial  Lundy murders 

  5. References

  6. External links

Stephen Andrew Bustin (born 1954)[1] is a British scientist, former professor of molecular sciences at Queen Mary University of London from 2004 to 2012, as well as visiting professor at Middlesex University, beginning in 2006.[2][3] In 2012 he was appointed Professor of Allied Health and Medicine at Anglia Ruskin University.[4] He is known for his research into polymerase chain reaction, and has written a book on the topic, entitled A-Z of Quantitative PCR. This book has been called "the bible of qPCR."[5]

Education

Bustin obtained his B.A. and PhD from Trinity College, Dublin in molecular genetics.[6]

Career

Following the merger with St Bartholomew's Medical College and Queen Mary University of London, Bustin was promoted to Reader in Molecular Medicine in 2002, followed by the award of a personal chair as Professor of Molecular Science in 2004 at Barts and The London School of Medicine and Dentistry.[7] {{As of|2015}}, Bustin held the position of Professor of Molecular Medicine at Anglia Ruskin University.[8]{{rp|219}} He is a fellow of the Society of Biology.[9]

Bustin also co-founded and edits the journal Biomolecular Detection and Quantification to provide a peer-reviewed outlet for "high-quality quantitative studies".[8]{{rp|219}}[10]

Research

His research group’s general areas of interest are the small and large bowel, as well as colorectal cancer with particular emphasis on investigating the process of invasion and metastasis. An important aim is to translate molecular techniques into clinical practice by including molecular parameters into clinical tumor staging.[11] To this end, Bustin has published many papers on PCR techniques,[12][13] in particular reverse transcription polymerase chain reaction, the subject of his most cited paper, published in 2000.[14]

He also developed the MIQE guidelines in a 2009 paper published in Clinical Chemistry,[15] the goal of which is to create guidelines for how PCR should be performed to ensure that PCR results are being reliably conducted and interpreted, as well as to make replication of experiments easier.

This paper is the fifth most cited one ever to be published in Clinical Chemistry,[16][17] with over 1700 citations on Google Scholar as of September 2013.[18]

Testimony

Autism omnibus trial

Bustin testified on behalf of the Department of Justice in the autism omnibus trial about what he stated was the unreliability of the O'Leary lab's results with regard to testing for contamination. The lab had claimed to find measles virus in the intestines of children with developmental disorders.[19] Bustin describes his conclusions with regard to the lab's alleged detection of measles virus RNA as follows: "My clear conclusion then was that O'Leary's results were caused by defective experimental technique and inappropriate interpretation of results, since he was detecting DNA, and measles virus does not exist as DNA."[20][21] Bustin was described as "one of the most highly qualified and credible expert witnesses I [the Special Master] have ever encountered."[4] In addition to his testimony, Bustin published an analysis of Andrew Wakefield's 2002 study, which had been published in the journal Molecular Pathology. This analysis, like Bustin's testimony, concluded that "The only conclusion possible is that the assays were detecting contaminating DNA. Since MeV is an RNA-only virus and never exists in DNA form, these data must be ignored and it is my opinion that the authors should withdraw this publication from the peer-reviewed literature."[22][23]

Lundy murders

Bustin testified in the trials pertaining to the Lundy murders in 2015, criticizing tests that had claimed to detect human brain cells on Mark Lundy's shirt.[24][25]

References

1. ^{{cite web|url=https://www.researchgate.net/profile/Stephen_Bustin2/info/|title=Stephen Bustin|work=ResearchGate|accessdate=24 September 2013}}
2. ^{{cite web|url=http://selectbiosciences.com/conferences/biographies.aspx?speaker=25335&conf=QPCRA2012|title=Stephen Bustin's Biography|publisher=Selectbiosciences|date=2011|accessdate=12 August 2013}}
3. ^{{cite web|url=http://www.dddmag.com/articles/2007/10/how-reliable-your-qpcr-data|title=How Reliable is Your qPCR Data?|work=Drug Discovery & Development|date=10 May 2007|accessdate=12 December 2013|author=McGee, Patrick}}
4. ^1 {{cite web|url=http://www.qpcrexpert.com/|title=Definitive qPCR|date=2013|accessdate=12 August 2013|author=Bustin, Stephen}}
5. ^{{cite web|url=http://www.the-scientist.com/?articles.view/articleNo/38422/title/PCR--Past--Present----Future/|title=PCR: Past, Present, & Future|work=The Scientist|date=1 December 2013|accessdate=21 February 2014|author=Perkel, Jeffrey M.}}
6. ^{{cite web|url=http://www.anglia.ac.uk/medical-science/about/our-staff/stephen-bustin|title=Stephen Bustin|work=Anglia Ruskin University|accessdate=30 September 2016}}
7. ^{{cite journal|url=http://www.badscience.net/wp-content/uploads/erp_mmr.pdf|title=RT-qPCR and molecular diagnostics: no evidence for measles virus in the GI tract of autistic children|author=Bustin, Stephen|journal=European Pharmaceutical Review|year=2008|issue=1|pages=11–17}}
8. ^1 {{cite journal|last1=Perkel|first1=Jeffrey|title=GUIDING OUR PCR EXPERIMENTS|journal=BioTechniques|date=1 May 2015|volume=58|issue=5|pages=217–21|doi=10.2144/000114283|pmid=25967899}}
9. ^{{Cite web |url=https://www.anglia.ac.uk/medical-science/about/our-staff/stephen-bustin |title=Professor Stephen Bustin |website=Anglia Ruskin University |language=en |access-date=2018-01-18}}
10. ^{{cite book|url=http://www.journals.elsevier.com/biomolecular-detection-and-quantification/|title=Biomolecular Detection and Quantification|publisher=Elsevier|accessdate=30 August 2015}}
11. ^{{cite web|url=http://webinar.sciencemag.org/webinar/archive/future-qpcr|title=The future of qPCR|publisher=American Association for the Advancement of Science|date=30 September 2010|accessdate=11 August 2013}}
12. ^{{Cite journal|last1=Nolan|first1=T.|last2=Hands|first2=R. E.|last3=Bustin|first3=S. A.|doi=10.1038/nprot.2006.236|title=Quantification of mRNA using real-time RT-PCR|journal=Nature Protocols|volume=1|issue=3|pages=1559–1582|year=2006|pmid=17406449|pmc=}}
13. ^{{Cite journal|last1=Mueller|first1=R.|last2=Bustin|first2=S. A.|doi=10.1042/CS20050086|title=Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis|journal=Clinical Science|volume=109|issue=4|pages=365–379|year=2005|pmid=16171460|pmc=|citeseerx=10.1.1.328.8607}}
14. ^{{Cite journal|last1=Bustin|first1=S.|title=Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays|doi=10.1677/jme.0.0250169|journal=Journal of Molecular Endocrinology|volume=25|issue=2|pages=169–193|year=2000|pmid=11013345|pmc=}}
15. ^{{Cite journal|last1=Bustin|first1=S. A.|last2=Benes|first2=V.|last3=Garson|first3=J. A.|last4=Hellemans|first4=J.|last5=Huggett|first5=J.|last6=Kubista|first6=M.|last7=Mueller|first7=R.|last8=Nolan|first8=T.|last9=Pfaffl|first9=M. W.|last10=Shipley|first10=G. L.|last11=Vandesompele|first11=J.|last12=Wittwer|first12=C. T.|doi=10.1373/clinchem.2008.112797|title=The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments|journal=Clinical Chemistry|volume=55|issue=4|pages=611–622|year=2009|pmid=19246619|pmc=}}
16. ^{{cite web|url=http://www.miqe.info/|title=The MIQE Guidelines|accessdate=11 August 2013|author=}}
17. ^{{cite web|url=http://www.clinchem.org/reports/most-cited|title=Most Cited Articles|work=Clinical Chemistry|accessdate=9 September 2013}}
18. ^https://scholar.google.com/citations?view_op=view_citation&hl=en&user=F62lTaUAAAAJ&citation_for_view=F62lTaUAAAAJ:d1gkVwhDpl0C
19. ^{{cite journal|title=PCR: living life amplified and standardized|journal=Nature|date=2013|author=Marx, Vivien|volume=10|issue=5|doi=10.1038/nmeth.2443|pmid = 23629414|pages=391–395}}
20. ^{{cite web|url=https://www.theguardian.com/science/2008/dec/09/medical-research-mmr-vaccine|title=Fading Claims of MMR Link to Autism|publisher=The Guardian|date=8 December 2008|accessdate=11 August 2013|author=Bustin, Stephen}}
21. ^{{cite web|url=http://www.spiked-online.com/newsite/article/3562#.UqqNEWRDuAA|title='The MMR-autism theory? There's nothing in it'|work=Spiked Online|date=4 July 2007|accessdate=12 December 2013|author=Fitzpatrick, Michael}}
22. ^{{Cite book|last1=Bustin|first1=S. A.|chapter=Why There Is no Link Between Measles Virus and Autism|doi=10.5772/52844|title=Recent Advances in Autism Spectrum Disorders - Volume I|year=2013|isbn=978-953-51-1021-7|pmid=|pmc=}}
23. ^{{cite book|title=A History of Autism: Conversations with the Pioneers|publisher=Blackwell Publishing|author=Feinstein, Adam|year=2010|pages=227}}
24. ^{{cite news|url=http://www.nzherald.co.nz/nz/news/article.cfm?c_id=1&objectid=11415544|title=Mark Lundy retrial: DNA on shirt matched to victim|work=New Zealand Herald|date=11 March 2015|accessdate=30 August 2015|author=Quilliam, Rebecca}}
25. ^{{cite web|url=http://www.stuff.co.nz/national/crime/67123673/Mark-Lundy-murder-retrial-Day-20|title=Mark Lundy murder retrial: Day 20|work=Stuff.co.nz|date=9 March 2015|accessdate=30 August 2015|author=Galuszka, Jono}}

External links

  • {{Google Scholar id|F62lTaUAAAAJ}}
{{Authority control}}{{DEFAULTSORT:Bustin, Stephen}}

7 : 1954 births|Academics of Anglia Ruskin University|Academics of Queen Mary University of London|Alumni of Trinity College Dublin|Autism researchers|British molecular biologists|Living people
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