| 词条 | Transposon tagging |
| 释义 |
In genetic engineering, transposon tagging is a process where transposons (transposable elements) are amplified inside a biological cell by a tagging technique. Transposon tagging has been used with several species to isolate genes.[1] Even without knowing the nature of the specific genes, the process can still be used.[1] Transposon tagging in plantsBy molecular separation of transposons, from a cell nucleus, the cloning is enabled for genes which contain the transposons.[2] By using transposon tagging, researchers have been able to add genetic elements from maize (corn)[3] and Antirrhinum into some other species (such as tobacco,[4] aspen[5] and others).[2] A gene responsible for a particular phenotype can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype.[2] Further reading
References1. ^1 {{cite web |first=Paaras V. |last=Shah |year=2007 |title=Transposable Elements |url=http://arabidopsis.info/students/paaras/transposable_elements.htm}}{{Self-published inline|date=March 2011}} {{genetics-stub}}2. ^1 2 {{cite web |title=Transposon Tagging |first=Phillip |last=McClean |year=1998 |url=http://www.ndsu.edu/pubweb/~mcclean/plsc731/transposon/tag4.htm}}{{Self-published inline|date=March 2011}} 3. ^{{cite journal |last1=Brutnell |first1=Thomas |title=Transposon tagging in maize |journal=Functional & Integrative Genomics |volume=2 |issue=1-2 |pages=4–12 |year=2002 |pmid=12021846 |doi=10.1007/s10142-001-0044-0}} 4. ^{{cite journal |doi=10.1073/pnas.92.10.4175 |last1=Dinesh-Kumar |first1=S. P. |last2=Whitham |first2=S. |last3=Choi |first3=D. |last4=Hehl |first4=R. |last5=Corr |first5=C. |last6=Baker |first6=B. |title=Transposon Tagging of Tobacco Mosaic Virus Resistance Gene N: Its Possible Role in the TMV-N-Mediated Signal Transduction Pathway |journal=Proceedings of the National Academy of Sciences of the United States of America |volume=92 |issue=10 |pages=4175–4180 |year=1995 |pmid=7753780 |pmc=41906 |jstor=2367289}} 5. ^{{cite journal |last1=Fladung |first1=M |last2=Deutsch |first2=F |last3=Hönicka |first3=H |last4=Kumar |first4=S |title=T-DNA and Transposon Tagging in Aspen |journal=Plant Biology |volume=6 |issue=1 |pages=5–11 |year=2004 |pmid=15095129 |doi=10.1055/s-2003-44745 }} 2 : Genetic engineering|Cloning |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。