| 词条 | Transposable element |
| 释义 |
A transposable element (TE or transposon) is a DNA sequence that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size.[1] Transposition often results in duplication of the same genetic material. Barbara McClintock's discovery of these jumping genes earned her a Nobel Prize in 1983.[2] Transposable elements make up a large fraction of the genome and are responsible for much of the mass of DNA in a eukaryotic cell. It has been shown that TEs are important in genome function and evolution.[3] In Oxytricha, which has a unique genetic system, these elements play a critical role in development.[4] Transposons are also very useful to researchers as a means to alter DNA inside a living organism. There are at least two classes of TEs: Class I TEs or retrotransposons generally function via reverse transcription, while Class II TEs or DNA transposons encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins.[5] DiscoveryBarbara McClintock discovered the first TEs in maize (Zea mays) at the Cold Spring Harbor Laboratory in New York. McClintock was experimenting with maize plants that had broken chromosomes.[6]In the winter of 1944–1945, McClintock planted corn kernels that were self-pollinated, meaning that the silk (style) of the flower received pollen from its own anther.[6] These kernels came from a long line of plants that had been self-pollinated, causing broken arms on the end of their ninth chromosomes.[6] As the maize plants began to grow, McClintock noted unusual color patterns on the leaves.[6] For example, one leaf had two albino patches of almost identical size, located side by side on the leaf.[6] McClintock hypothesized that during cell division certain cells lost genetic material, while others gained what they had lost.[7] However, when comparing the chromosomes of the current generation of plants with the parent generation, she found certain parts of the chromosome had switched position.[7] This refuted the popular genetic theory of the time that genes were fixed in their position on a chromosome. McClintock found that genes could not only move, but they could also be turned on or off due to certain environmental conditions or during different stages of cell development.[7] McClintock also showed that gene mutations could be reversed.[8] She presented her report on her findings in 1951, and published an article on her discoveries in Genetics in November 1953 entitled "Induction of Instability at Selected Loci in Maize".[9] Her work was largely dismissed and ignored until the late 1960s–1970s when, after TEs were found in bacteria, it was rediscovered.[10] She was awarded a Nobel Prize in Physiology or Medicine in 1983 for her discovery of TEs, more than thirty years after her initial research.[11] Approximately 90% of the maize genome is made up of TEs,[12][13] as is 44% of the human genome.[14] ClassificationTransposable elements represent one of several types of mobile genetic elements. TEs are assigned to one of two classes according to their mechanism of transposition, which can be described as either copy and paste (Class I TEs) or cut and paste (Class II TEs).[15] Retrotransposon{{Main|Retrotransposon}}Class I TEs are copied in two stages: first, they are transcribed from DNA to RNA, and the RNA produced is then reverse transcribed to DNA. This copied DNA is then inserted back into the genome at a new position. The reverse transcription step is catalyzed by a reverse transcriptase, which is often encoded by the TE itself. The characteristics of retrotransposons are similar to retroviruses, such as HIV. Retrotransposons are commonly grouped into three main orders:
(Retroviruses can also be considered TEs. For example, after conversion of retroviral RNA into DNA inside a host cell, the newly produced retroviral DNA is integrated into the genome of the host cell. These integrated DNAs are termed proviruses. The provirus is a specialized form of eukaryotic retrotransposon, which can produce RNA intermediates that may leave the host cell and infect other cells. The transposition cycle of retroviruses has similarities to that of prokaryotic TEs, suggesting a distant relationship between the two.) DNA transposonsThe cut-and-paste transposition mechanism of class II TEs does not involve an RNA intermediate. The transpositions are catalyzed by several transposase enzymes. Some transposases non-specifically bind to any target site in DNA, whereas others bind to specific target sequences. The transposase makes a staggered cut at the target site producing sticky ends, cuts out the DNA transposon and ligates it into the target site. A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This results in target site duplication and the insertion sites of DNA transposons may be identified by short direct repeats (a staggered cut in the target DNA filled by DNA polymerase) followed by inverted repeats (which are important for the TE excision by transposase). Cut-and-paste TEs may be duplicated if their transposition takes place during S phase of the cell cycle, when a donor site has already been replicated but a target site has not yet been replicated.[17] Such duplications at the target site can result in gene duplication, which plays an important role in genomic evolution.[18]{{rp|284}} Not all DNA transposons transpose through the cut-and-paste mechanism. In some cases, a replicative transposition is observed in which a transposon replicates itself to a new target site (e.g. helitron). Class II TEs comprise less than 2% of the human genome, making the rest Class I.[19] Autonomous and non-autonomousTransposition can be classified as either "autonomous" or "non-autonomous" in both Class I and Class II TEs. Autonomous TEs can move by themselves, whereas non-autonomous TEs require the presence of another TE to move. This is often because dependent TEs lack transposase (for Class II) or reverse transcriptase (for Class I). Activator element (Ac) is an example of an autonomous TE, and dissociation elements (Ds) is an example of a non-autonomous TE. Without Ac, Ds is not able to transpose. Examples
In diseaseTEs are mutagens and their movements are often the causes of genetic disease. They can damage the genome of their host cell in different ways:[31]
Diseases often caused by TEs include hemophilia A and B, severe combined immunodeficiency, porphyria, predisposition to cancer, and Duchenne muscular dystrophy.[32][33] LINE1 (L1) TEs that land on the human Factor VIII have been shown to cause haemophilia[34] and insertion of L1 into the APC gene causes colon cancer, confirming that TEs play an important role in disease development.[35] Transposable element dysregulation can cause neuronal death in Alzheimer's disease and similar tauopathies.[36] Additionally, many TEs contain promoters which drive transcription of their own transposase. These promoters can cause aberrant expression of linked genes, causing disease or mutant phenotypes. Rate of transposition, induction and defenseOne study estimated the rate of transposition of a particular retrotransposon, the Ty1 element in Saccharomyces cerevisiae. Using several assumptions, the rate of successful transposition event per single Ty1 element came out to be about once every few months to once every few years.[37] Some TEs contain heat-shock like promoters and their rate of transposition increases if the cell is subjected to stress,[38] thus increasing the mutation rate under these conditions, which might be beneficial to the cell. Cells defend against the proliferation of TEs in a number of ways. These include piRNAs and siRNAs,[39] which silence TEs after they have been transcribed. If organisms are mostly composed of TEs, one might assume that disease caused by misplaced TEs is very common, but in most cases TEs are silenced through epigenetic mechanisms like DNA methylation, chromatin remodeling and piRNA, such that little to no phenotypic effects nor movements of TEs occur as in some wild-type plant TEs. Certain mutated plants have been found to have defects in methylation-related enzymes (methyl transferase) which cause the transcription of TEs, thus affecting the phenotype.[5][40] One hypothesis suggests that only approximately 100 LINE1 related sequences are active, despite their sequences making up 17% of the human genome. In human cells, silencing of LINE1 sequences is triggered by an RNA interference (RNAi) mechanism. Surprisingly, the RNAi sequences are derived from the 5' untranslated region (UTR) of the LINE1, a long terminal which repeats itself. Supposedly, the 5' LINE1 UTR that codes for the sense promoter for LINE1 transcription also encodes the antisense promoter for the miRNA that becomes the substrate for siRNA production. Inhibition of the RNAi silencing mechanism in this region showed an increase in LINE1 transcription.[5][41] EvolutionTEs are found in almost all life forms, and the scientific community is still exploring their evolution and their effect on genome evolution. It is unclear whether TEs originated in the last universal common ancestor, arose independently multiple times, or arose once and then spread to other kingdoms by horizontal gene transfer.[42] While some TEs confer benefits on their hosts, most are regarded as selfish DNA parasites. In this way, they are similar to viruses. Various viruses and TEs also share features in their genome structures and biochemical abilities, leading to speculation that they share a common ancestor.[43] Because excessive TE activity can damage exons, many organisms have acquired mechanisms to inhibit their activity. Bacteria may undergo high rates of gene deletion as part of a mechanism to remove TEs and viruses from their genomes, while eukaryotic organisms typically use RNA interference to inhibit TE activity. Nevertheless, some TEs generate large families often associated with speciation events. Evolution often deactivates DNA transposons, leaving them as introns (inactive gene sequences). In vertebrate animal cells, nearly all 100,000+ DNA transposons per genome have genes that encode inactive transposase polypeptides.[44] The first synthetic transposon designed for use in vertebrate (including human) cells, the Sleeping Beauty transposon system, is a Tc1/mariner-like transposon. Its dead ("fossil") versions are spread widely in the salmoid genome and a functional version was engineered by comparing those versions.[45] Human Tc1-like transposons are divided into Hsmar1 and Hsmar2 subfamilies. Although both types are inactive, one copy of Hsmar1 found in the SETMAR gene is under selection as it provides DNA-binding for the histone-modifying protein.[46] Many other human genes are similarly derived from transposons.[47] Hsmar2 has been reconstructed multiple times from the fossil sequences.[48] Large quantities of TEs within genomes may still present evolutionary advantages, however. Interspersed repeats within genomes are created by transposition events accumulating over evolutionary time. Because interspersed repeats block gene conversion, they protect novel gene sequences from being overwritten by similar gene sequences and thereby facilitate the development of new genes. TEs may also have been co-opted by the vertebrate immune system as a means of producing antibody diversity. The V(D)J recombination system operates by a mechanism similar to that of some TEs. TEs can contain many types of genes, including those conferring antibiotic resistance and ability to transpose to conjugative plasmids. Some TEs also contain integrons, genetic elements that can capture and express genes from other sources. These contain integrase, which can integrate gene cassettes. There are over 40 antibiotic resistance genes identified on cassettes, as well as virulence genes. Transposons do not always excise their elements precisely, sometimes removing the adjacent base pairs; this phenomenon is called exon shuffling. Shuffling two unrelated exons can create a novel gene product or, more likely, an intron.[49] Applications{{Main|Transposons as a genetic tool}}The first TE was discovered in maize (Zea mays) and is named dissociator (Ds). Likewise, the first TE to be molecularly isolated was from a plant (snapdragon). Appropriately, TEs have been an especially useful tool in plant molecular biology. Researchers use them as a means of mutagenesis. In this context, a TE jumps into a gene and produces a mutation. The presence of such a TE provides a straightforward means of identifying the mutant allele relative to chemical mutagenesis methods. Sometimes the insertion of a TE into a gene can disrupt that gene's function in a reversible manner, in a process called insertional mutagenesis; transposase-mediated excision of the DNA transposon restores gene function. This produces plants in which neighboring cells have different genotypes. This feature allows researchers to distinguish between genes that must be present inside of a cell in order to function (cell-autonomous) and genes that produce observable effects in cells other than those where the gene is expressed. TEs are also a widely used tool for mutagenesis of most experimentally tractable organisms. The Sleeping Beauty transposon system has been used extensively as an insertional tag for identifying cancer genes.[50] The Tc1/mariner-class of TEs Sleeping Beauty transposon system, awarded Molecule of the Year in 2009,[51] is active in mammalian cells and is being investigated for use in human gene therapy.[52][53][54] TEs are used for the reconstruction of phylogenies by the means of presence/absence analyses.[55] transposons can act as biological mutagen in bacteria De novo repeat identificationDe novo repeat identification is an initial scan of sequence data that seeks to find the repetitive regions of the genome, and to classify these repeats. Many computer programs exist to perform de novo repeat identification, all operating under the same general principles.[64] As short tandem repeats are generally 1–6 base pairs in length and are often consecutive, their identification is relatively simple.[56] Dispersed repetitive elements, on the other hand, are more challenging to identify, due to the fact that they are longer and have often acquired mutations. However, it is important to identify these repeats as they are often found to be transposable elements (TEs).[57]De novo identification of transposons involves three steps: 1) find all repeats within the genome, 2) build a consensus of each family of sequences, and 3) classify these repeats. There are three groups of algorithms for the first step. One group is referred to as the k-mer approach, where a k-mer is a sequence of length k. In this approach, the genome is scanned for overrepresented k-mers; that is, k-mers that occur more often than is likely based on probability alone. The length k is determined by the type of transposon being searched for. The k-mer approach also allows mismatches, the number of which is determined by the analyst. Some k-mer approach programs use the k-mer as a base, and extend both ends of each repeated k-mer until there is no more similarity between them, indicating the ends of the repeats.[57] Another group of algorithms employs a method called sequence self-comparison. Sequence self-comparison programs use databases such as AB-BLAST to conduct an initial sequence alignment. As these programs find groups of elements that partially overlap, they are useful for finding highly diverged transposons, or transposons with only a small region copied into other parts of the genome.[58] Another group of algorithms follows the periodicity approach. These algorithms perform a Fourier transformation on the sequence data, identifying periodicities, regions that are repeated periodically, and are able to use peaks in the resultant spectrum to find candidate repetitive elements. This method works best for tandem repeats, but can be used for dispersed repeats as well. However, it is a slow process, making it an unlikely choice for genome scale analysis.[57]The second step of de novo repeat identification involves building a consensus of each family of sequences. A consensus sequence is a sequence that is created based on the repeats that comprise a TE family. A base pair in a consensus is the one that occurred most often in the sequences being compared to make the consensus. For example, in a family of 50 repeats where 42 have a T base pair in the same position, the consensus sequence would have a T at this position as well, as the base pair is representative of the family as a whole at that particular position, and is most likely the base pair found in the family's ancestor at that position.[57] Once a consensus sequence has been made for each family, it is then possible to move on to further analysis, such as TE classification and genome masking in order to quantify the overall TE content of the genome. Adaptive TEsTransposable elements have been recognized as good candidates for stimulating gene adaptation, through their ability to regulate the expression levels of nearby genes.[59] Combined with their "mobility", transposable elements can be relocated adjacent to their targeted genes, and control the expression levels of the gene, dependent upon the circumstances. The study conducted in 2008, "High Rate of Recent Transposable Element–Induced Adaptation in Drosophila melanogaster", used D. melanogaster that had recently migrated from Africa to other parts of the world, as a basis for studying adaptations caused by transposable elements. Although most of the TEs were located on introns, the experiment showed the significant difference on gene expressions between the population in Africa and other parts of the world. The four TEs that caused the selective sweep were more prevalent in D. melanogaster from temperate climates, leading the researchers to conclude that the selective pressures of the climate prompted genetic adaptation.[60] From this experiment, it has been confirmed that adaptive TEs are prevalent in nature, by enabling organisms to adapt gene expression as a result of new selective pressures. However, not all effects of adaptive TEs are beneficial to the population. In the research conducted in 2009, "A Recent Adaptive Transposable Element Insertion Near Highly Conserved Developmental Loci in Drosophila melanogaster", a TE, inserted between Jheh 2 and Jheh 3, revealed a downgrade in the expression level of both of the genes. Down regulation of such genes has caused Drosophila to exhibit extended developmental time and reduced egg to adult viability. Although this adaptation was observed in high frequency in all non-African populations, it was not fixed in any of them.[61] This is not hard to believe, since it is logical for a population to favor higher egg to adult viability, therefore trying to purge the trait caused by this specific TE adaptation. At the same time, there have been several reports showing the advantageous adaptation caused by TEs. In the research done with silkworms, "An Adaptive Transposable Element insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm", a TE insertion was observed in the cis-regulatory region of the EO gene, which regulates molting hormone 20E, and enhanced expression was recorded. While populations without the TE insert are often unable to effectively regulate hormone 20E under starvation conditions, those with the insert had a more stable development, which resulted in higher developmental uniformity.[62] These three experiments all demonstrated different ways in which TE insertions can be advantageous or disadvantageous, through means of regulating the expression level of adjacent genes. The field of adaptive TE research is still under development and more findings can be expected in the future. See also
Notes
References1. ^{{cite journal | vauthors = Bourque G, Burns KH, Gehring M, Gorbunova V, Seluanov A, Hammell M, Imbeault M, Izsvák Z, Levin HL, Macfarlan TS, Mager DL, Feschotte C | title = Ten things you should know about transposable elements | journal = Genome Biology | volume = 19 | issue = 1 | page = 199 | date = November 2018 | pmid = 30454069 | pmc = 6240941 | doi = 10.1186/s13059-018-1577-z }} 2. ^{{cite journal | vauthors = McCLINTOCK B | title = The origin and behavior of mutable loci in maize | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 36 | issue = 6 | pages = 344–55 | date = June 1950 | pmid = 15430309 | pmc = 1063197 | doi = 10.1073/pnas.36.6.344 | bibcode = 1950PNAS...36..344M }} 3. ^{{cite journal | vauthors = Bucher E, Reinders J, Mirouze M | title = Epigenetic control of transposon transcription and mobility in Arabidopsis | journal = Current Opinion in Plant Biology | volume = 15 | issue = 5 | pages = 503–10 | date = November 2012 | pmid = 22940592 | doi = 10.1016/j.pbi.2012.08.006 }} 4. ^{{cite web |url=https://www.sciencedaily.com/releases/2009/05/090520140408.htm |title='Junk' DNA Has Important Role, Researchers Find |work=Science Daily |date=21 May 2009}} 5. ^1 2 {{cite journal | last =Pray | first =Leslie A. | title =Transposons: The jumping genes | journal =Nature Education | volume =1 | issue =1 | page =204 | year =2008 | url =http://www.nature.com/scitable/topicpage/transposons-the-jumping-genes-518}} 6. ^1 2 3 4 {{cite book |first=Sharon Bertsch |last=McGrayne |title=Nobel Prize Women in Science: Their Lives, Struggles, and Momentous Discoveries |url=https://books.google.com/books?id=e7NaAAAAYAAJ |year=1998 |publisher=Carol Publishing |isbn=978-0-9702256-0-3 |edition=2nd |ref=harv |page=165}} 7. ^1 2 {{harnvb|McGrayne|1998|p=166}} 8. ^{{harnvb|McGrayne|1998|p=167}} 9. ^{{cite journal | vauthors = McClintock B | title = Induction of Instability at Selected Loci in Maize | journal = Genetics | volume = 38 | issue = 6 | pages = 579–99 | date = November 1953 | pmid = 17247459 | pmc = 1209627 | url = http://www.genetics.org/cgi/pmidlookup?view=long&pmid=17247459 }} 10. ^{{cite book |first=Julie |last=Des Jardins |title=The Madame Curie Complex: The Hidden History of Women in Science |url=https://books.google.com/books?id=HULGDNDSenYC&pg=PA246 |year=2010 |publisher=Feminist Press at CUNY |isbn=978-1-55861-655-4 |page=246}} 11. ^{{cite book |editor-first=Nina |editor-last=Fedoroff |editor2-first=David |editor2-last=Botstein |title=The Dynamic Genome: Barbara McClintock's Ideas in the Century of Genetics |url=https://books.google.com/books?id=BF1xfIGd0b4C&pg=PA2 |date=1 January 1992 |publisher=Cold Spring Harbor Laboratory Press |isbn=978-0-87969-422-7 |page=2}} 12. ^{{cite journal | vauthors = SanMiguel P, Tikhonov A, Jin YK, Motchoulskaia N, Zakharov D, Melake-Berhan A, Springer PS, Edwards KJ, Lee M, Avramova Z, Bennetzen JL | display-authors = 6 | title = Nested retrotransposons in the intergenic regions of the maize genome | journal = Science | volume = 274 | issue = 5288 | pages = 765–8 | date = November 1996 | pmid = 8864112 | doi = 10.1126/science.274.5288.765 }} 13. ^{{cite journal | vauthors = Jiao Y, Peluso P, Shi J, Liang T, Stitzer MC, Wang B, Campbell MS, Stein JC, Wei X, Chin CS, Guill K, Regulski M, Kumari S, Olson A, Gent J, Schneider KL, Wolfgruber TK, May MR, Springer NM, Antoniou E, McCombie WR, Presting GG, McMullen M, Ross-Ibarra J, Dawe RK, Hastie A, Rank DR, Ware D | display-authors = 6 | title = Improved maize reference genome with single-molecule technologies | journal = Nature | volume = 546 | issue = 7659 | pages = 524–527 | date = June 2017 | pmid = 28605751 | doi = 10.1038/nature22971 | bibcode = 2017Natur.546..524J }} 14. ^{{cite journal | vauthors = Mills RE, Bennett EA, Iskow RC, Devine SE | title = Which transposable elements are active in the human genome? | journal = Trends in Genetics | volume = 23 | issue = 4 | pages = 183–91 | date = April 2007 | pmid = 17331616 | doi = 10.1016/j.tig.2007.02.006 }} 15. ^{{cite journal | vauthors = Kapitonov VV, Jurka J | title = A universal classification of eukaryotic transposable elements implemented in Repbase | journal = Nature Reviews. Genetics | volume = 9 | issue = 5 | pages = 411–2; author reply 414 | date = May 2008 | pmid = 18421312 | doi = 10.1038/nrg2165-c1 }} 16. ^{{Cite thesis |last=Walter |first=Marius |year=2016 |title=Transposon regulation upon dynamic loss of DNA methylation |url=http://rgdoi.net/10.13140/RG.2.2.18747.21286|publisher=Université Pierre et Marie Curie|doi=10.13140/rg.2.2.18747.21286}} 17. ^{{cite journal |last1=Young |display-authors=et al |title=Review of techniques and methods in replication and hybridization of transposable elements in vitro |journal=Journal of Biomolecular Technology |date=2012 |volume=19 |issue=18 |pages=341–357}} 18. ^{{cite book |veditors=Madigan M, Martinko J |title=Brock Biolog of Microorganisms |edition=11th |publisher=Prentice Hall |year=2006 |isbn=978-0-13-144329-7}} 19. ^{{cite journal | vauthors = Kazazian HH, Moran JV | title = The impact of L1 retrotransposons on the human genome | journal = Nature Genetics | volume = 19 | issue = 1 | pages = 19–24 | date = May 1998 | pmid = 9590283 | doi = 10.1038/ng0598-19 }} 20. ^{{cite journal | vauthors = Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK | display-authors = 6 | title = The B73 maize genome: complexity, diversity, and dynamics | journal = Science | volume = 326 | issue = 5956 | pages = 1112–5 | date = November 2009 | pmid = 19965430 | doi = 10.1126/science.1178534 | bibcode = 2009Sci...326.1112S }} 21. ^{{cite journal | vauthors = Spradling AC, Rubin GM | title = Transposition of cloned P elements into Drosophila germ line chromosomes | journal = Science | volume = 218 | issue = 4570 | pages = 341–7 | date = October 1982 | pmid = 6289435 | doi = 10.1126/science.6289435 | bibcode = 1982Sci...218..341S }} 22. ^{{cite journal | vauthors = Rubin GM, Spradling AC | title = Genetic transformation of Drosophila with transposable element vectors | journal = Science | volume = 218 | issue = 4570 | pages = 348–53 | date = October 1982 | pmid = 6289436 | doi = 10.1126/science.6289436 | bibcode = 1982Sci...218..348R }} 23. ^{{cite journal |vauthors=Cesari F |title=Milestones in Nature: Milestone 9: Transformers, Elements in Disguise |journal=Nature |date=15 October 2007 |doi=10.1038/nrg2254 |url=http://www.nature.com/milestones/miledna/full/miledna09.html}} 24. ^{{cite journal | vauthors = Kazazian HH, Moran JV | title = The impact of L1 retrotransposons on the human genome | journal = Nature Genetics | volume = 19 | issue = 1 | pages = 19–24 | date = May 1998 | pmid = 9590283 | doi = 10.1038/ng0598-19 }} 25. ^{{cite journal | vauthors = Jacobson JW, Medhora MM, Hartl DL | title = Molecular structure of a somatically unstable transposable element in Drosophila | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 83 | issue = 22 | pages = 8684–8 | date = November 1986 | pmid = 3022302 | pmc = 386995 | doi = 10.1073/pnas.83.22.8684 | bibcode = 1986PNAS...83.8684J }} 26. ^{{cite journal | vauthors = Lohe AR, Moriyama EN, Lidholm DA, Hartl DL | title = Horizontal transmission, vertical inactivation, and stochastic loss of mariner-like transposable elements | journal = Molecular Biology and Evolution | volume = 12 | issue = 1 | pages = 62–72 | date = January 1995 | pmid = 7877497 | doi = 10.1093/oxfordjournals.molbev.a040191 }} 27. ^{{cite journal | vauthors = Lampe DJ, Witherspoon DJ, Soto-Adames FN, Robertson HM | title = Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer | journal = Molecular Biology and Evolution | volume = 20 | issue = 4 | pages = 554–62 | date = April 2003 | pmid = 12654937 | doi = 10.1093/molbev/msg069 }} 28. ^{{cite journal | vauthors = Mandal PK, Kazazian HH | title = SnapShot: Vertebrate transposons | journal = Cell | volume = 135 | issue = 1 | pages = 192–192.e1 | date = October 2008 | pmid = 18854165 | doi = 10.1016/j.cell.2008.09.028 }} 29. ^{{cite journal | vauthors = Carlton JM, Hirt RP, Silva JC, Delcher AL, Schatz M, Zhao Q, Wortman JR, Bidwell SL, Alsmark UC, Besteiro S, Sicheritz-Ponten T, Noel CJ, Dacks JB, Foster PG, Simillion C, Van de Peer Y, Miranda-Saavedra D, Barton GJ, Westrop GD, Müller S, Dessi D, Fiori PL, Ren Q, Paulsen I, Zhang H, Bastida-Corcuera FD, Simoes-Barbosa A, Brown MT, Hayes RD, Mukherjee M, Okumura CY, Schneider R, Smith AJ, Vanacova S, Villalvazo M, Haas BJ, Pertea M, Feldblyum TV, Utterback TR, Shu CL, Osoegawa K, de Jong PJ, Hrdy I, Horvathova L, Zubacova Z, Dolezal P, Malik SB, Logsdon JM, Henze K, Gupta A, Wang CC, Dunne RL, Upcroft JA, Upcroft P, White O, Salzberg SL, Tang P, Chiu CH, Lee YS, Embley TM, Coombs GH, Mottram JC, Tachezy J, Fraser-Liggett CM, Johnson PJ | display-authors = 6 | title = Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis | journal = Science | volume = 315 | issue = 5809 | pages = 207–12 | date = January 2007 | pmid = 17218520 | pmc = 2080659 | doi = 10.1126/science.1132894 | bibcode = 2007Sci...315..207C }} 30. ^{{cite journal | vauthors = Kim JM, Vanguri S, Boeke JD, Gabriel A, Voytas DF | title = Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence | journal = Genome Research | volume = 8 | issue = 5 | pages = 464–78 | date = May 1998 | pmid = 9582191 | doi = 10.1101/gr.8.5.464 }} 31. ^{{cite journal | vauthors = Belancio VP, Hedges DJ, Deininger P | title = Mammalian non-LTR retrotransposons: for better or worse, in sickness and in health | journal = Genome Research | volume = 18 | issue = 3 | pages = 343–58 | date = March 2008 | pmid = 18256243 | doi = 10.1101/gr.5558208 }} 32. ^{{cite journal | vauthors = Kazazian HH, Goodier JL | title = LINE drive. retrotransposition and genome instability | journal = Cell | volume = 110 | issue = 3 | pages = 277–80 | date = August 2002 | pmid = 12176313 | doi = 10.1016/S0092-8674(02)00868-1 }} 33. ^{{Cite book |vauthors=Kapitonov VV, Pavlicek A, Jurka J |year=2006 |title=Anthology of Human Repetitive DNA |journal=Encyclopedia of Molecular Cell Biology and Molecular Medicine |doi=10.1002/3527600906.mcb.200300166|isbn=978-3527600908 }} 34. ^{{cite journal | vauthors = Kazazian HH, Wong C, Youssoufian H, Scott AF, Phillips DG, Antonarakis SE | title = Haemophilia A resulting from de novo insertion of L1 sequences represents a novel mechanism for mutation in man | journal = Nature | volume = 332 | issue = 6160 | pages = 164–6 | date = March 1988 | pmid = 2831458 | doi = 10.1038/332164a0 | bibcode = 1988Natur.332..164K }} 35. ^{{cite journal | vauthors = Miki Y, Nishisho I, Horii A, Miyoshi Y, Utsunomiya J, Kinzler KW, Vogelstein B, Nakamura Y | title = Disruption of the APC gene by a retrotransposal insertion of L1 sequence in a colon cancer | journal = Cancer Research | volume = 52 | issue = 3 | pages = 643–5 | date = February 1992 | pmid = 1310068 }} 36. ^{{cite journal | vauthors = Sun W, Samimi H, Gamez M, Zare H, Frost B | title = Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies | journal = Nature Neuroscience | volume = 21 | issue = 8 | pages = 1038–1048 | date = August 2018 | pmid = 30038280 | pmc = 6095477 | doi = 10.1038/s41593-018-0194-1 }} 37. ^{{cite journal | vauthors = Paquin CE, Williamson VM | title = Temperature effects on the rate of ty transposition | journal = Science | volume = 226 | issue = 4670 | pages = 53–5 | date = October 1984 | pmid = 17815421 | doi = 10.1126/science.226.4670.53 | bibcode = 1984Sci...226...53P }} 38. ^{{cite journal | vauthors = Strand DJ, McDonald JF | title = Copia is transcriptionally responsive to environmental stress | journal = Nucleic Acids Research | volume = 13 | issue = 12 | pages = 4401–10 | date = June 1985 | pmid = 2409535 | pmc = 321795 | doi = 10.1093/nar/13.12.4401 }} 39. ^{{cite journal | vauthors = Chung WJ, Okamura K, Martin R, Lai EC | title = Endogenous RNA interference provides a somatic defense against Drosophila transposons | journal = Current Biology | volume = 18 | issue = 11 | pages = 795–802 | date = June 2008 | pmid = 18501606 | pmc = 2812477 | doi = 10.1016/j.cub.2008.05.006 }} 40. ^{{cite journal | vauthors = Miura A, Yonebayashi S, Watanabe K, Toyama T, Shimada H, Kakutani T | title = Mobilization of transposons by a mutation abolishing full DNA methylation in Arabidopsis | journal = Nature | volume = 411 | issue = 6834 | pages = 212–4 | date = May 2001 | pmid = 11346800 | doi = 10.1038/35075612 }} 41. ^{{cite journal | vauthors = Yang N, Kazazian HH | title = L1 retrotransposition is suppressed by endogenously encoded small interfering RNAs in human cultured cells | journal = Nature Structural & Molecular Biology | volume = 13 | issue = 9 | pages = 763–71 | date = September 2006 | pmid = 16936727 | doi = 10.1038/nsmb1141 }} 42. ^{{cite journal | vauthors = Kidwell MG | title = Horizontal transfer of P elements and other short inverted repeat transposons | journal = Genetica | volume = 86 | issue = 1-3 | pages = 275–86 | year = 1992 | pmid = 1334912 | doi = 10.1007/BF00133726 }} 43. ^{{cite book |vauthors=Villarreal L |year=2005 |title=Viruses and the Evolution of Life |location=Washington |publisher=ASM Press}} 44. ^{{cite journal | vauthors = Plasterk RH, Izsvák Z, Ivics Z | title = Resident aliens: the Tc1/mariner superfamily of transposable elements | journal = Trends in Genetics | volume = 15 | issue = 8 | pages = 326–32 | date = August 1999 | pmid = 10431195 | doi = 10.1016/S0168-9525(99)01777-1 }} 45. ^{{cite journal | vauthors = Ivics Z, Hackett PB, Plasterk RH, Izsvák Z | title = Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells | journal = Cell | volume = 91 | issue = 4 | pages = 501–10 | date = November 1997 | pmid = 9390559 | doi = 10.1016/S0092-8674(00)80436-5 }} 46. ^{{cite journal |last1=Miskey |first1=C. |last2=Papp |first2=B. |last3=Mates |first3=L. |last4=Sinzelle |first4=L. |last5=Keller |first5=H. |last6=Izsvak |first6=Z. |last7=Ivics |first7=Z. |title=The Ancient mariner Sails Again: Transposition of the Human Hsmar1 Element by a Reconstructed Transposase and Activities of the SETMAR Protein on Transposon Ends |journal=Molecular and Cellular Biology |date=2 April 2007 |volume=27 |issue=12 |pages=4589–4600 |doi=10.1128/MCB.02027-06|pmc=1900042 }} 47. ^{{cite web |title=Gene group: Transposable element derived genes |url=https://www.genenames.org/data/genegroup/#!/group/1416 |publisher=HUGO Gene Nomenclature Committee |accessdate=4 March 2019}} 48. ^{{cite journal |last1=Gil |first1=Estel |last2=Bosch |first2=Assumpcio |last3=Lampe |first3=David |last4=Lizcano |first4=Jose M. |last5=Perales |first5=Jose C. |last6=Danos |first6=Olivier |last7=Chillon |first7=Miguel |last8=Cordaux |first8=Richard |title=Functional Characterization of the Human Mariner Transposon Hsmar2 |journal=PLoS ONE |date=11 September 2013 |volume=8 |issue=9 |pages=e73227 |doi=10.1371/journal.pone.0073227}} 49. ^{{cite journal | vauthors = Moran JV, DeBerardinis RJ, Kazazian HH | title = Exon shuffling by L1 retrotransposition | journal = Science | volume = 283 | issue = 5407 | pages = 1530–4 | date = March 1999 | pmid = 10066175 | doi = 10.1126/science.283.5407.1530 | bibcode = 1999Sci...283.1530M }} 50. ^{{cite journal | vauthors = Carlson CM, Largaespada DA | title = Insertional mutagenesis in mice: new perspectives and tools | journal = Nature Reviews. Genetics | volume = 6 | issue = 7 | pages = 568–80 | date = July 2005 | pmid = 15995698 | doi = 10.1038/nrg1638 }} 51. ^{{cite journal | vauthors = Luft FC | title = Sleeping Beauty jumps to new heights | journal = Journal of Molecular Medicine | volume = 88 | issue = 7 | pages = 641–3 | date = July 2010 | pmid = 20467721 | doi = 10.1007/s00109-010-0626-1 }} 52. ^{{cite journal | vauthors = Ivics Z, Izsvák Z | title = Transposons for gene therapy! | journal = Current Gene Therapy | volume = 6 | issue = 5 | pages = 593–607 | date = October 2006 | pmid = 17073604 | doi = 10.2174/156652306778520647 }} 53. ^{{cite journal | vauthors = Wilson MH, Coates CJ, George AL | title = PiggyBac transposon-mediated gene transfer in human cells | journal = Molecular Therapy | volume = 15 | issue = 1 | pages = 139–45 | date = January 2007 | pmid = 17164785 | doi = 10.1038/sj.mt.6300028 }} 54. ^{{cite journal | vauthors = Hackett PB, Largaespada DA, Cooper LJ | title = A transposon and transposase system for human application | journal = Molecular Therapy | volume = 18 | issue = 4 | pages = 674–83 | date = April 2010 | pmid = 20104209 | pmc = 2862530 | doi = 10.1038/mt.2010.2 }} 55. ^{{cite journal | vauthors = Kriegs JO, Churakov G, Kiefmann M, Jordan U, Brosius J, Schmitz J | title = Retroposed elements as archives for the evolutionary history of placental mammals | journal = PLoS Biology | volume = 4 | issue = 4 | page = e91 | date = April 2006 | pmid = 16515367 | pmc = 1395351 | doi = 10.1371/journal.pbio.0040091 }} 56. ^{{cite journal |vauthors=Saha S, Bridges S, Magbanua ZV, Peterson DG |title=Computational Approaches and Tools Used in Identification of Dispersed Repetitive DNA Sequences |journal=Tropical Plant Biol. |volume=1 |issue= |pages=85–96 |year=2008 |doi=10.1007/s12042-007-9007-5 }} 57. ^1 2 3 4 {{Cite book |vauthors=Makałowski W, Pande A, Gotea V, Makałowska I |title=Transposable elements and their identification |journal=Methods Mol. Biol. |volume=855 |pages=337–59 |year=2012 |pmid=22407715 |doi=10.1007/978-1-61779-582-4_12 |series=Methods in Molecular Biology |isbn=978-1-61779-581-7 }} 58. ^{{cite journal | vauthors = Saha S, Bridges S, Magbanua ZV, Peterson DG | title = Empirical comparison of ab initio repeat finding programs | journal = Nucleic Acids Research | volume = 36 | issue = 7 | pages = 2284–94 | date = April 2008 | pmid = 18287116 | pmc = 2367713 | doi = 10.1093/nar/gkn064 }} 59. ^{{cite journal | vauthors = Mariño-Ramírez L, Lewis KC, Landsman D, Jordan IK | title = Transposable elements donate lineage-specific regulatory sequences to host genomes | journal = Cytogenetic and Genome Research | volume = 110 | issue = 1-4 | pages = 333–41 | year = 2005 | pmid = 16093685 | pmc = 1803082 | doi = 10.1159/000084965 | lastauthoramp = y }} 60. ^{{cite journal | vauthors = González J, Lenkov K, Lipatov M, Macpherson JM, Petrov DA | title = High rate of recent transposable element-induced adaptation in Drosophila melanogaster | journal = PLoS Biology | volume = 6 | issue = 10 | pages = e251 | date = October 2008 | pmid = 18942889 | pmc = 2570423 | doi = 10.1371/journal.pbio.0060251 | lastauthoramp = y }} 61. ^{{cite journal | vauthors = González J, Macpherson JM, Petrov DA | title = A recent adaptive transposable element insertion near highly conserved developmental loci in Drosophila melanogaster | journal = Molecular Biology and Evolution | volume = 26 | issue = 9 | pages = 1949–61 | date = September 2009 | pmid = 19458110 | pmc = 2734154 | doi = 10.1093/molbev/msp107 | lastauthoramp = y }} 62. ^{{cite journal | vauthors = Sun W, Shen YH, Han MJ, Cao YF, Zhang Z | title = An adaptive transposable element insertion in the regulatory region of the EO gene in the domesticated silkworm, Bombyx mori | journal = Molecular Biology and Evolution | volume = 31 | issue = 12 | pages = 3302–13 | date = December 2014 | pmid = 25213334 | doi = 10.1093/molbev/msu261 | lastauthoramp = y }} External links
2 : Molecular biology|Mobile genetic elements |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。