| 词条 | XPG I protein domain |
| 释义 |
| Symbol = XPG_I | Name = XPG_I | image = PDB 1a77 EBI.jpg | width = | caption = flap endonuclease-1 from methanococcus jannaschii | Pfam = PF00867 | Pfam_clan = CL0464 | InterPro = IPR006086 | SMART = | PROSITE = PDOC00658 | MEROPS = | SCOP = 1a77 | TCDB = | OPM family = | OPM protein = | CAZy = | CDD = cd09868 }} In molecular biology, the XPG-I is a protein domain found on Xeroderma Pigmentosum Complementation Group G (XPG) protein.[1] The XPG protein is an endonuclease which repairs DNA damage caused by ultraviolet light (UV light). The XPG protein repairs DNA by a process called, Nucleotide excision repair. Mutations in the protein commonly cause Xeroderma Pigmentosum which often lead to skin cancer. FunctionThe function of the internal XPG (XPG-I) domain contains many of cysteine and glutamate amino acid residues that are frequently found in various enzyme active sites, DNA nucleases. The I domain, together with the N-terminal forms the catalytic domain that contains the active site.[2] MechanismXPG cleaves the 5'-overhanging flap structure that is generated when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It has both 5'endo-/exonuclease and 5'-pseudo-Y-endonuclease activities. Cleaves the junction between single and double-stranded regions of flap DNA. The endonuclease binds 2 magnesium ions per subunit, which probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding. References1. ^{{cite journal|vauthors=O'Donovan A, Scherly D, Clarkson SG, Wood RD | title=Isolation of active recombinant XPG protein, a human DNA repair endonuclease. | journal=J Biol Chem | year= 1994 | volume= 269 | issue= 23 | pages= 15965–8 | pmid=8206890 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8206890 }} {{InterPro content|IPR006086}}2. ^{{cite journal| author=Clarkson SG| title=The XPG story. | journal=Biochimie | year= 2003 | volume= 85 | issue= 11 | pages= 1113–21 | pmid=14726017 | doi= 10.1016/j.biochi.2003.10.014| pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14726017 }} 3 : Protein families|Protein domains|DNA repair |
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