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词条 Y187
释义

  1. References

Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech[1] since at least 2000 and is used as a partner with strain AH109 in mating assays.[2]

Genotype of Y187: MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, 112, gal4Δ, met–, gal80Δ, URA3::GAL1UAS-GAL1TATA-lacZ.[3]

References

1. ^{{cite journal|last=Fromont-Racine|first=Micheline |author2=Rain, Jean-Christophe |author3=Legrain, Pierre|title=Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens|journal=Nature Genetics|year=1997|volume=16|issue=3|pages=277–282|doi=10.1038/ng0797-277|pmid=9207794}}
2. ^{{cite journal|last=Guo|first=Deyin |author2=Rajamäki, Minna-Liisa |author3=Valkonen, Jari|title=Protein–Protein Interactions: The Yeast Two-Hybrid System|journal=Methods in Molecular Biology|year=2008|volume=451|series=Plant Virology Protocols|issue=3|pages=421–439|doi=10.1007/978-1-59745-102-4_29}}
3. ^http://www.uni-jena.de/unijenamedia/Downloads/faculties/bio_pharm/ls_genetik/Teaching/Grundpraktikum/Y2Hscript_SS11.pdf{{dead link|date=July 2016 |bot=InternetArchiveBot |fix-attempted=yes }}
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1 : Yeasts

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