“ADA (buffer)”的意思、由来-开放百科全书

词条

ADA (buffer)

释义

References

{{chembox
| Name = ADA
| ImageFile = ADA (buffer).png
| ImageName = Chemical structure of ADA
| IUPACName = 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid^[1]
| OtherNames = ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid
|Section1={{Chembox Identifiers
| InChI = 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
| InChIKey = QZTKDVCDBIDYMD-UHFFFAOYSA-N
| SMILES = C(C(=O)N)N(CC(=O)O)CC(=O)O
| CASNo = 26239-55-4
| UNII = 5C4R3O704E
| EC_number = 247-530-0
| PubChem = 117765
| Section2 = {{Chembox Properties
| C=6 | H=10 | N=2 | O=5
| MolarMass = 190.155 g/mol
| pKa = 6.6
| Section3 = {{Chembox Hazards
| MainHazards = Irritant
| FlashPt = Non-flammable
| GHSSignalWord = Warning
| HPhrases = {{H-phrases|302|315|319|335}}
| PPhrases = {{P-phrases|261|264|270|271|280|301+312|302+352|304+340|305+351+338|312|321|330|332+313|337+313|362|403+233|405|501}}
}}

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H⁺, Ca²⁺, and Mg²⁺, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na⁺/K⁺-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.^[2]

References

1. ^{{cite web| title=Compound Summary for CID 117765| website=PubChem| date=2005-03-26| url=https://pubchem.ncbi.nlm.nih.gov/compound/N-_2-Acetamido_iminodiacetic_acid#section=Top}}
2. ^{{cite web| title=ADA| website=Sigma-Aldrich| url=http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a9883pis.pdf}}

4 : Zwitterions|Amines|Tricarboxylic acids|Buffers

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