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词条 Cell spreader
释义

  1. Uses

      Sterilization    After uses  

  2. References

{{more footnotes|date=September 2017}}

A cell spreader is a tool used in the biological field to smoothly spread cells and bacteria on a plate or a Petri dish. Spreaders can come in three different shapes: the triangular shape, the L-shape, and the T-shape. Spreaders can be made from glass, plastic, or metal, depending on their function.

Uses

Drop cells or bacteria at the center of the dish. Researchers can then place the spreader on top of the dish and, without applying much pressure, swirl the spreader around on the dish to evenly distribute the cells or bacteria.[1]

Sterilization

Before using a cell spreader, if the spreader is made from glass or metal, researchers must sterilize the spreader by submerging it in alcohol or ethanol and later burning the alcohol off by placing the spreader in a Bunsen burner flame to eliminate microorganisms.[2]

After uses

After each use, the spreader should be back in alcohol or ethanol to keep it clean and prevent the spreader to contaminated with unwanted particles.

For disposable cell spreaders made of plastic, which are only used once, not necessarily sterilized and then discard them after uses.

References

1. ^{{Cite web|url=http://www.chemistry.ucla.edu/sites/default/files/safety/sop/SOP_Spreading_Bacterial_Cultures.pdf|title=Standard Operating Procedure|last=|first=|date=|website=|archive-url=|archive-date=|dead-url=|access-date=}}
2. ^{{Cite web|url=https://www.phys.ksu.edu/gene/g1.html|title=Laboratory Methods|website=www.phys.ksu.edu|access-date=2017-03-09}}
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