| School | Location | Athletics | Ref |
|---|
| Albright College | Pennsylvania | Division III | [2] |
| Ashland University | Ohio | Division II |
| Averett University | Virginia | Division III |
| Becker College | Massachusetts | Division III |
| Bellevue University | Nebraska | NAIA |
| Benedictine University at Mesa | Arizona | NAIA |
| Boise State University | Idaho | Division I |
| Brescia University | Kentucky | NAIA |
| Campbellsville University | Kentucky | NAIA |
| Central Methodist University | Missouri | NAIA |
| Centralia College | Washington | NWAC |
| Cincinnati Christian University | Ohio | NAIA |
| Coker College | South Carolina | Division II |
| College of St. Joseph | Vermont | Division II/NAIA |
| Columbia College | Missouri | NAIA |
| Culver-Stockton College | Missouri | NAIA |
| Daemen College | New York | Division II |
| Defiance College | Ohio | Division III |
| Dickinson State University | North Dakota | NAIA |
| DigiPen Institute of Technology | Washington | N/A |
| ECPI University | Virginia | [https://nacesports.org/school-directory/ecpi-university// NACE] |
| Embry Riddle Aeronautical University | Arizona | NAIA |
| Fontbonne University | Missouri | Division III |
| Florida Southern College | Florida | Division II |
| Georgia Southern University | Georgia | Division I |
| Georgia State University | Georgia | Division I |
| Grand View University | Iowa | NAIA |
| Harrisburg University | Pennsylvania | N/A |
| Hawkeye Community College | Iowa | NJCAA |
| Illinois College | Illinois | Division III |
| Illinois Wesleyan University | Illinois | Division III |
| Indiana Institute of Technology | Indiana | NAIA |
| Kansas Wesleyan University | Kansas | NAIA |
| Kent State University | Ohio | Division I |
| King University | Tennessee | Division II |
| Lambton College | Ontario | OCAA |
| Lebanon Valley College | Pennsylvania | Division III |
| Lees-McRae College | North Carolina | Division II |
| Lourdes University | Ohio | NAIA |
| Maryville University | Missouri | Division II |
| McPherson College | Kansas | NAIA |
| Menlo College | California | NAIA |
| Miami University | Ohio | Division I |
| Midland University | Nebraska | NAIA |
| Missouri Baptist University | Missouri | NAIA |
| Morningside College | Iowa | NAIA |
| Northern Virginia Community College | Virginia | NJCAA |
| Northwest Christian University | Oregon | NAIA |
| Oregon Institute of Technology | Oregon | NAIA |
| Pratt Community College | Kansas | NJCAA |
| Principia College | Illinois | Division III |
| Robert Morris University Illinois | Illinois | NAIA |
| Schreiner University | Texas | Division III |
| St. Louis College of Pharmacy | Missouri | NAIA |
| South Dakota School of Mines & Technology | South Dakota | Division II |
| Southwestern College | Kansas | NAIA |
| Southwest Baptist University | Missouri | Division II |
| St. Ambrose University | Iowa | NAIA |
| St. Clair College | Ontario | OCAA |
| St. Thomas Aquinas College | New York | Division II |
| Stephens College | Missouri | NAIA |
| Stevenson University | Maryland | Division III |
| SUNY Canton | New York | Division III |
| Tennessee Technological University | Tennessee | Division I |
| University of Texas Dallas | Texas | Division III |
| Texas Wesleyan University | Texas | NAIA |
| Tiffin University | Ohio | Division II |
| Trine University | Indiana | Division III |
| University of Akron | Ohio | Division I |
| University of California-Berkeley | California | Division I |
| University of California-Irvine | California | Division I |
| University of Jamestown | North Dakota | NAIA |
| University of North Texas | Texas | Division I |
| University of Pikeville | Kentucky | NAIA |
| University of Providence | Montana | NAIA |
| University of South Carolina-Sumter | South Carolina | NJCAA |
| University of Utah | Utah | Division I | [3] |
| West Virginia Wesleyan College | West Virginia | Division II |
| Western Kentucky University | Kentucky | Division I |
1. ^{{Cite news |url=http://www.espn.com/esports/story/_/id/21152905/college-esports-list-varsity-esports-programs-north-america|title=List of varsity esports programs spans North America|work=ESPN.com|access-date=2018-11-27|language=en}}
2. ^{{cite web|url=https://www.albright.edu/2018/10/01/esports-to-be-added-as-albright-varsity-sport/|title=eSports to be added as Albright varsity sport|date=October 1, 2018|website=Albright College|accessdate=24 March 2019}}
3. ^{{cite web|url=https://www.utahbusiness.com/new-home-team-varsity-gaming-university-utah/|title=The New Home Team: Varsity Gaming at the University of Utah|last=Foreman|first=Kelsie|date=February 26, 2018|website=Utah Business|accessdate=24 November 2018}}