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词条 Czapek medium
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  1. References

  2. External links

Czapek medium, also called Czapek's agar (CZA)[1][2] or Czapek-Dox medium, is a growth medium for propagating fungi and other organisms in a laboratory. It was named after its inventors, Polish botanist Friedrich Johann Franz Czapek (May 16, 1868 - July 31, 1921) and American chemist Arthur Wayland Dox (September 19, 1882 - 1954). It was developed to grow Aspergillus niger[3] and Penicillium camemberti.[4] It works well for many saprophytic fungi and soil bacteria[5] such as species of Aspergillus, Candida, Penicillium, and Paecilomyces.[3]Friedrich Czapek's original recipe is as follows:[4]
  • 1000 g distilled water
  • 30 g cane sugar – energy source and sole source of carbon[5]
  • 1 g monopotassium phosphate – buffering agent[5]
  • 0.5 g magnesium sulfate – source of cations[5]
  • 0.5 g potassium chloride – source of essential ions[5]
  • 0.01 g iron sulfate – source of cations[5]

Arthur Wayland Dox added 2 g of sodium nitrate in his version, to provide a sole source of nitrogen that is inorganic.[6] This makes the medium a selective growth medium as only organisms that can use inorganic nitrogen can grow.[3] Czapek and Dox did not add agar but many recipes add 15 g to make a solid medium.[5][3]

References

1. ^{{cite web|author1=|title=Czapek Agar (CZA) Recipe|url=http://www.thelabrat.com/protocols/CzapekAgarRecipe.shtml|website=theLabRat.com|publisher=theLabRat.com|accessdate=7 November 2017|language=English|date=2005|quote=Czapek Agar (CZA) Recipe}}
2. ^{{cite web|author1=|title=NRRL Medium No. 8 Czapek's Solution Agar ( CZA )|url=https://nrrl.ncaur.usda.gov/forms/NRRL_Medium_8.pdf|website=ARS (NRRL) Culture Collection|publisher=ARS Culture Collection National Center for Agricultural Utilization Research|accessdate=7 November 2017|location=Peoria, IL|language=English|format=PDF|quote=Czapek’s Solution Agar ( CZA )}}
3. ^1 2 {{cite web|author1=|title=CZAPEK-DOX AGAR|url=https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Czapek-DoxAgar.html|website=Hardy Diagnostics Instructions for Use|publisher=Hardy Diagnostics|accessdate=3 October 2017|location=Santa Maria, CA|language=English}}
4. ^1 {{cite journal |last= Czapek |first=F |title= Untersuchungen über die Stickstoffgewinnung und Eiweifsbildung der Pflanzen |trans-title= Studies on nitrogen production and protein formation of plants |journal= Beiträge zur chemischen Physiologie und Pathologie |date= 1901–1902 |volume=1 |issue=12 |pages= 538–560 |url= https://www.biodiversitylibrary.org/page/39459974|accessdate= 2 October 2017 |language= German |format=PDF |oclc= 1519369}}
5. ^1 2 3 4 5 6 {{cite web|author1=|title=CZAPEK MEDIUM|url=https://tools.thermofisher.com/content/sfs/manuals/IFU9322.pdf|website=Thermo Fisher Scientific|publisher=Remel|accessdate=3 October 2017|location=Lenexa, KS|language=English|format=PDF|date=September 20, 2007}}
6. ^1 {{cite journal|last1=Dox|first1=Arthur Wayland|title=The intracellular enzyms of penicillium and aspergillus: with special reference to those of Penicillium camemberti|journal=Bulletin (United States Bureau of Animal Industry)|url=https://books.google.com/?id=iOS7chsvBwQC&lpg=PA1&dq=Dox%20USDA%20Bureau%20Animal%20Bulletin%20120&pg=PA38#v=onepage&q=potassium&f=false|date=1910|volume=120|page=37|accessdate=3 October 2017|language=English|format=PDF|oclc=22281943}}

External links

  • [https://tools.thermofisher.com/content/sfs/manuals/IFU9322.pdf Czapek Medium recipe]
  • [https://wiki.bugwood.org/Czapek_solution_agar Czapek solution agar recipe]
  • [https://www.atcc.org/~/media/F3504E41D5BB48718DF559C7C334DA94.ashx Czapek's Agar recipe]
  • [https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Czapek-DoxAgar.html Czapek-Dox Agar]
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2 : Microbiological media|Cell culture media
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