“Deepak T. Nair”的意思、由来-开放百科全书

Biography

His parents are from the southern state of Kerala and he was born in Pune in the western state of Maharashtra on 25 October 1973,^[3] Deepak Nair went to school at the Jai Hind High School (Pimpri) and then later to the St. Vincents Junior College (Pune). He graduated with B.Sc in Chemistry from Fergusson College (1994) and completed his Masters in Biotechnology from the Savitribai Phule Pune University (1996).^[4] Subsequently, he enrolled for his doctoral studies at the National Institute of Immunology, India to secure a PhD in structural immunology in 2001. For his PhD he worked under the supervision of Dinakar Mashnu Salunke. Later, he moved to the US to complete his post-doctoral work in the laboratory of Aneel K. Aggarwal at the Mount Sinai Medical Center. He returned to India in 2007 to take up the position of an independent investigator at the National Centre for Biological Sciences .^[5] He worked in NCBS as Reader-F (2007-2013) and Associate Professor (2013-2014). In July, 2014, he joined the Regional Centre for Biotechnology as an Associate professor, a post he holds to date.^[4]

Research

Deepak Nair has obtained new insight regarding the molecular mechanisms that determine the fidelity of the replication process in bacteria and flaviviruses. He has discovered the mechanism employed by DNA polymerase IV to rescue replication stalled at damaged nucleotides with unprecedented efficiency and accuracy (Structure, 2014, 23:56-67). Nair has provided insight into how specialized DNA polymerases that participate in adaptive mutagenesis ensure achieve function (Nucleic Acids Research, 2013, 41:5104-5014; Acta Crystallogr D Biol Crystallogr. 2012 68:960-7, J Nucleic Acids. 2012:285481). His laboratory has shown how GTP binding to the viral RNA-dependent-RNA polymerase ensures accurate initiation of replication of the viral genome (Nucleic Acids Research, 2014, 42:2758-2573). In addition, he has shown that reactive oxygen species do play an important role in the antimicrobial activity of bactericidal antibiotics (Angew Chem Int Ed Engl. 2016 55:2397-400). In collaboration with D. N. Rao (Department of Biochemistry, IISc), his laboratory has also contributed towards understanding how proteins involved in post-replicative repair of DNA mismatches function (Nucleic Acids Research, 2017, 46:256-266; PLoS One. 2010, 5:e13726)

As a post-doctoral fellow (Dec 2001- July 2007) he focused on understanding the structural basis of DNA lesion bypass by eukaryotic Y-family DNA polymerases using X-ray crystallography. Due to the action of a variety of agents, lesions are formed on DNA which interfere with normal replication and may also prove carcinogenic. Eukaryotes possess up to four specialized DNA polymerases that are able to synthesize DNA across these lesions and thus prevent the replication fork from stalling. Nair determined the crystal structure of the catalytic cores of two such polymerases, human DNA polymerase iota (hPolι) and yeast REV1 (yREV1) –in complex with DNA and incoming nucleotide. The structures of hPolι and yRev1 in complex with undamaged and damaged DNA has shown that these two polymerases prefer altered modes of base-pairing in the active site to facilitate lesion bypass (Nature, 2004, 430:377; Science, 2005, 309:2219; Structure, 2005, 13:1569; Structure, 2006, 14:749; Nat. Struct. Mol. Biol., 2006, 13:619; Structure, 2008, 16:239; Structure, 2009, 17:530). Both hPolι and yREV1 have unique active sites that facilitate the formation of non-Watson-Crick base pairs to achieve lesion bypass and rescue stalled replication. He also played a role in the determination of the structure of a third Y-family polymerase human DNA Polymerase kappa in its functional state (Mol. Cell, 2007, 25:601). In addition, he also participated in projects aimed at understanding the nature of interactions between the translational regulator Pumilio and non-cognate RNA targets (Structure, 2008, 16:549) and discerning the preference of hPolι for incorporating dGTP when the base of the templating nucleotide is thymine (Structure, 2009, 17:974).

His doctoral thesis (July 1996- Dec 2001) describes the crystallographic analysis of a panel of three murine monoclonal antibodies raised against the same promiscuous peptide antigen PS1 (HQLDPAFGANSTNPD). (J Immunol, 2000, 165:6949; J. Immunol, 2002, 168:2371). The comparison of the structure of the antibodies in their bound and unbound state suggests there could be a convergence of both epitope and paratope conformations in an antibody response against a flexible immunodominant epitope (J. Immunol, 2002, 168:2371). He also carried out a computational analysis of the conformational propensities of native and retro-inverso versions of B-cell and T-cell epitopes (J. Immunol, 2003, 170:1362). This study showed that conformational and functional mimicry can be achieved through retro-inversion only if the native peptide is present in a linear extended conformation in its functional state. He was also involved in the structure determination of an antibacterial protein from tasar silkworm Antheraea mylitta (J. Biol. Chem., 2001, 276:41377). In addition, he modeled the complex of the ribonuclease restrictocin and its rRNA substrate (Biochemistry, 2001, 40:9115).

Awards and honors

Nair was selected for the Ramanujan Fellowship by the Department of Biotechnology in 2008, the tenure of the fellowship running until 2013,^[5] and he became a member of the Guha Research Conference in 2013. He received the National Bioscience Award for Career Development (N-BIOS Prize) in 2014.^[6] The Council of Scientific and Industrial Research awarded him the Shanti Swarup Bhatnagar Prize, one of the highest Indian science awards in 2017.^[2]

Publications

See also

Notes

1. ^{{Cite web |url=http://ssbprize.gov.in/Content/Detail.aspx?AID=535 |title=View Bhatnagar Awardees |date=2017 |publisher=Shanti Swarup Bhatnagar Prize |access-date=11 November 2017}}
2. ^¹{{Cite web |url=http://www.thehindu.com/news/national/10-scientists-receive-shanti-swarup-bhatnagar-prize/article19758442.ece |title=10 scientists receive Shanti Swarup Bhatnagar Prize |date=26 September 2017 |publisher=The Hindu |access-date=2017-11-11}}
3. ^{{Cite web |url=http://ssbprize.gov.in/Content/Detail.aspx?AID=528 |title=Brief Profile of the Awardee |date=2017-10-21 |publisher=Shanti Swarup Bhatnagar Prize |access-date=2017-10-21}}
4. ^¹{{Cite web |url=http://www.rcb.res.in/deepak |title=Biographical Information - Deepak Nair |date=2017-11-09 |website=Regional Centre for Biotechnology |access-date=2017-11-09}}
5. ^¹{{Cite web |url=http://serb.gov.in/pdfs/research1/Deepak_Thankappan_Nair.pdf |title=Profile on SERB |date=2017-11-14 |website=Science and Engineering Research Board |access-date=2017-11-14}}
6. ^{{Cite web |url=http://www.dbtindia.nic.in/wp-content/uploads/NationalBioScienceAwardCareerDevelopment2014.pdf |title=N-BIOS Prize 2014 |date=2014 |website=Department of Biotechnology |access-date=2017-11-14}}

References

External links