| 词条 | Draft:Sulfolobus islandicus Rod-Shaped Virus 2 |
| 释义 |
| image = | image_alt = | image_caption = | taxon = Sulfolobus islandicus rod-shaped virus 2 | authority = | synonyms = | synonyms_ref = | subdivision_ranks = | subdivision = }}Sulfolobus islandicus rod-shaped virus 2, also referred to as SIRV2, is a archeal virus that infects the archeaon Sulfolobus islandicus. This virus belongs to the family Rudiviridae.[1] Biology and biochemistrySIRV2 has a linear double-stranded DNA genome. The viral DNA is replicated by 4 host DNA polymerases: Dpo1 through Dpo4.[1] The virus has a rod-shaped morphology with a width of 23 nanometers (nm) and a length of 900 nm. The virus has terminal plugs that are pointed at one end and have a 45 nm length and 6 nm width. Three tails, 28 nm in length, have been observed on both ends of the virus.[2] SIRV2 is able to survive additions of 6 molar (M) urea, absolute ethanol, octanol-2, and 0.1% Triton X-100 in neutral pH and 25 degrees Celsius.[2] In vitro testing has shown that SIRV2 is still able to infect at 70-80 degrees Celsius and in a pH 3 solution.[3] SIRV2gp19 was found to be a single-stranded DNA endonuclease in 2011. This was proven by inducing a mutation in the SIRVgp19 protein Motif II from the amino acid aspartate to alanine which resulted in a loss of nuclease activity. This protein is functional within pH 7-10. Magnesium chloride was found to be a cofactor to this protein in 1971.[4][3] Sodium chloride concentrations above 100 mM inhibit SIRV2gp19.[3] References1. ^1 {{Cite journal|last=Martínez-Alvarez|first=Laura|last2=Deng|first2=Ling|last3=Peng|first3=Xu|date=2017-04-19|title=Formation of a Viral Replication Focus in Sulfolobus Cells Infected by the Rudivirus Sulfolobus islandicus Rod-Shaped Virus 2|journal=Journal of Virology|volume=91|issue=13|doi=10.1128/jvi.00486-17|pmid=28424282|pmc=5469267|issn=0022-538X}} Category:Rudiviridae2. ^1 {{Cite book|title=A novel virus family, the Rudiviridae: Structure, virus-host interactions and genome variability of the sulfolobus viruses SIRV1 and SIRV2.|last=W|first=Prangishvili, D Arnold, H P Götz, D Ziese, U Holz, I Kristjansson, J K Zillig|oclc=678661646}} 3. ^1 2 {{Cite journal|last=Gardner|first=Andrew F.|last2=Prangishvili|first2=David|last3=Jack|first3=William E.|date=2011-06-11|title=Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonuclease|journal=Extremophiles|volume=15|issue=5|pages=619–624|doi=10.1007/s00792-011-0385-0|pmid=21667093|pmc=3158340|issn=1431-0651}} 4. ^{{Cite journal|last=Kushner|first=S. R.|last2=Nagaishi|first2=H.|last3=Templin|first3=A.|last4=Clark|first4=A. J.|date=1971-04-01|title=Genetic Recombination in Escherichia coli: The Role of Exonuclease I|journal=Proceedings of the National Academy of Sciences|volume=68|issue=4|pages=824–827|doi=10.1073/pnas.68.4.824|issn=0027-8424}} |
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