“Draft:TRAFIX”的意思、由来-开放百科全书

TRAFIX.^[1] (TempoRAl Focusing microscopy with single-pIXel detection) is a wide-field multiphoton microscopy technique designed to image through scattering media. It uses a judicious combination of patterned temporal focusing illumination with single-pixel detection to obtain images of hidden fluorescent samples within or even beyond turbid media, namely tissue.

Working Principle

An expanded ultrashort pulsed laser beam illuminates a pattern generator (SLM or DMD) that sequentially creates orthonormal light patterns, typically in a Hadamard basis. The generated patterns are then projected onto a diffraction grating that spreads out its spectral components stretching the pulse in time. The grating is then imaged onto a fluorescent sample of interest using a 4F imaging system achieving temporal focusing^[2]^[3]. The outstanding ability of temporal focusing to retain the integrity of the illumination patterns through scattering^[4]^[5] makes it possible to deliver light very efficiently at depth. The effect of scattering on the emitted fluorescent photons is overcome by measuring the light intensity emitted by the sample under each illumination pattern with a single-pixel detector (typically a PMT or a binned EMCCD camera). In this way, TRAFIX can tolerate the scrambling of photons^[6] propagating back through the sample to the detector. Images can then be reconstructed by summing up the projected patterns weighted by the measured intensities in a scheme reminiscent of ghost imaging^[7]. Thanks to its patterned illumination nature, TRAFIX lends itself to compressive sensing^[8]

References

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