| 词条 | Flow-induced dispersion analysis |
| 释义 |
Flow-induced dispersion analysis (FIDA) is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions[1][2]. In the FIDA assay, the size of a ligand (indicator) with affinity to the target analyte is measured. When the indicator interacts with the analyte the apparent size increases and this change in size can be used to determine the analyte concentration and interaction[1][2][3]. Additionally, the hydrodynamic radius of the analyte-indicator complex is obtained. A FIDA assay is typically completed in minutes and only requires a modest sample consumption of a few µl[1]. Applications
PrincipleThe FIDA principle is based on measuring the change in the apparent size (diffusivity) of a selective indicator interacting with the analyte molecule[1][2][3]. The apparent indicator size is measured by Taylor dispersion analysis in a capillary under hydrodynamic flow[5]. References1. ^1 2 3 4 {{Cite journal|last=Poulsen|first=Nicklas N.|last2=Andersen|first2=Nina Z.|last3=Østergaard|first3=Jesper|last4=Zhuang|first4=Guisheng|last5=Petersen|first5=Nickolaj J.|last6=Jensen|first6=Henrik|date=2015-06-15|title=Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples|journal=The Analyst|language=en|volume=140|issue=13|pages=4365–4369|doi=10.1039/c5an00697j|pmid=26031223|issn=1364-5528}} 2. ^1 2 {{Cite book|title=Clinical Applications of Capillary Electrophoresis: Methods and Protocols|last=Morten E|first=Pedersen|last2=Østergaard|first2=Jesper|last3=Jensen|first3=Henrik|date=2019|publisher=Springer New York|isbn=9781493992126|editor-last=Phillips|editor-first=Terry M.|series=Methods in Molecular Biology|volume=1972|location=New York, NY|pages=109–123|chapter=Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization|doi=10.1007/978-1-4939-9213-3}} 3. ^1 {{Cite journal|last=Jensen|first=Henrik|last2=Østergaard|first2=Jesper|date=2010-03-31|title=Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples|journal=Journal of the American Chemical Society|volume=132|issue=12|pages=4070–4071|doi=10.1021/ja100484d|pmid=20201527|issn=0002-7863}} 4. ^{{Cite journal|last=Poulsen|first=Nicklas N.|last2=Pedersen|first2=Morten E.|last3=Østergaard|first3=Jesper|last4=Petersen|first4=Nickolaj J.|last5=Nielsen|first5=Christoffer T.|last6=Heegaard|first6=Niels H. H.|last7=Jensen|first7=Henrik|date=2016-09-20|title=Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients: Toward a New Approach for Diagnosis and Patient Stratification|journal=Analytical Chemistry|volume=88|issue=18|pages=9056–9061|doi=10.1021/acs.analchem.6b01741|pmid=27571264|issn=0003-2700}} 5. ^{{Cite journal|last=Taylor|first=Sir Geoffrey|last2=S|first2=F. R.|date=1953-08-25|title=Dispersion of soluble matter in solvent flowing slowly through a tube|url=http://rspa.royalsocietypublishing.org/content/219/1137/186|journal=Proc. R. Soc. Lond. A|language=en|volume=219|issue=1137|pages=186–203|doi=10.1098/rspa.1953.0139|issn=0080-4630}} 2 : Protein methods|Analytical chemistry |
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