“2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase”的意思、由来-开放百科全书

词条

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

释义

Structural studies
References

{{enzyme
| Name = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
| EC_number = 4.6.1.12
| CAS_number =
| IUBMB_EC_number = 4/6/1/12
| GO_code = 0008685
| image =
| width =
| caption =
}}

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MEcPP synthase, IspF, {{EC number|4.6.1.12}}) is a zinc-dependent enzyme which participates in the MEP pathway (non-mevalonate pathway) of isoprenoid precursor biosynthesis. It catalyzes the chemical reaction:

4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

Hence, this enzyme has one substrate, 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-MEP), and two products, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) and CMP.^[1]

The enzyme is considered a phosphorus-oxygen lyase. The systematic name of this enzyme class is 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include IspF, YgbB and MEcPP synthase.

Structural studies

As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes {{PDB link|1GX1}}, {{PDB link|1H47}}, {{PDB link|1H48}}, {{PDB link|1IV1}}, {{PDB link|1IV2}}, {{PDB link|1IV3}}, {{PDB link|1IV4}}, {{PDB link|1T0A}}, {{PDB link|1U3L}}, {{PDB link|1U3P}}, {{PDB link|1U40}}, {{PDB link|1U43}}, {{PDB link|1VH8}}, {{PDB link|1VHA}}, {{PDB link|1W55}}, {{PDB link|1W57}}, {{PDB link|1YQN}}, {{PDB link|2AMT}}, {{PDB link|2GZL}}, and {{PDB link|2PMP}}.

References

1. ^{{Cite journal|title = Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate to 2C-methyl-d-erythritol 2,4-cyclodiphosphate|vauthors=Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Lüttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F|date = 2000|journal = Proc. Natl. Acad. Sci. USA |issue=6 |doi = 10.1073/pnas.040554697|pmid = 10694574|access-date =|pmc=15955|volume=97|pages=2486–90}}

{{Non-mevalonate pathway enzymes}}{{Phosphorus-oxygen lyases}}{{Enzymes}}{{Portal bar|Molecular and Cellular Biology|border=no}}{{lyase-stub}}

2 : EC 4.6.1|Enzymes of known structure

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