| 词条 | 5.8S ribosomal RNA |
| 释义 |
| Name = 5.8S ribosomal RNA | image = RF00002.jpg | width = | caption = Predicted secondary structure and sequence conservation of 5.8S ribosomal RNA | Symbol = 5_8S_rRNA | AltSymbols = | Rfam = RF00002 | miRBase = | miRBase_family = | RNA_type = Gene; rRNA | Tax_domain = Eukaryota; Archaea | GO = {{GO|0003735}} {{GO|0005840}} | SO = {{SO|0000375}} | CAS_number = | EntrezGene = | HGNCid = | OMIM = | PDB = | RefSeq = | Chromosome = | Arm = | Band = | LocusSupplementaryData = }} In molecular biology, the 5.8S ribosomal RNA (5.8S rRNA) is a non-coding RNA component of the large subunit of the eukaryotic ribosome and so plays an important role in protein translation. It is transcribed by RNA polymerase I as part of the 45S precursor that also contains 18S and 28S rRNA. Its function is thought to be in ribosome translocation.[1] It is also known to form covalent linkage to the p53 tumour suppressor protein.[2] 5.8S rRNA can be used as a reference gene for miRNA detection.[3] The 5.8S ribosomal RNA is used to better understand other rRNA processes and pathways in the cell.[4] StructureL567.5 rRNA structure is approximately 150 nucleotides in size and it consists of plenty of folded strands, some of which are presumed to be single stranded.[5] This ribosomal RNA works in sync with the 28S rRNA molecule in various ways, i.e. in the folding of the 5.8S rRNA molecule structure.[6] The 5.8S and 28S rRNA have both an ITS 1 and ITS 2 (Internal transcribed spacer) and a 5’ and 3’ ETS (External transcribed spacer) that is between them.[7] The ITS and ETS are necessary because they must be cleaved in order for the 3’ end of the 5.8S rRNA to be formed; thus, maturing the rRNA. This is accomplished through a continuous cleavage pathway performed by both endonuclease and exonuclease enzymes, cutting the spacers at specific locations.[8] References1. ^{{cite journal | last = Abou | first = Elela S |author2= Nazar RN | year = 1997 | title = Role of the 5.8S rRNA in ribosome translocation | journal = Nucleic Acids Res | volume = 25 | pages = 1788–1794 | pmid = 9108162 | doi = 10.1093/nar/25.9.1788 | issue = 9 | pmc = 146658}} 2. ^{{cite journal | last = Fontoura | first = BM |author2=Atienza CA|author3=Sorokina EA|author4=Morimoto T|author5= Carroll RB | year = 1997 | title = Cytoplasmic p53 polypeptide is associated with ribosomes | journal = Mol Cell Biol | volume = 17 | pages = 3146–3154 | pmid = 9154813 | issue = 6 | pmc = 232167| doi = 10.1128/MCB.17.6.3146 }} 3. ^{{cite journal |author=Shi R, Chiang VL |title=Facile means for quantifying microRNA expression by real-time PCR |journal=BioTechniques |volume=39 |issue=4 |pages=519–25 |date=October 2005 |pmid=16235564 |doi=10.2144/000112010 |url=http://www.biotechniques.com/article/000112010 |archive-url=https://archive.is/20120731010516/http://www.biotechniques.com/article/000112010 |dead-url=yes |archive-date=2012-07-31 |accessdate=2010-09-15 }} 4. ^{{cite journal|last1=Nazar|first1=Ross N.|title=The ribosomal 5.8S RNA: eukaryotic adaptation or processing variant?|journal=Canadian Journal of Biochemistry and Cell Biology|date=1984|volume=62|issue=6|pages=311–320|doi=10.1139/o84-044}} 5. ^{{cite journal|last1=Walker|first1=Thomas A.|last2=Pace|first2=Norman R.|title=5.8S Ribosomal RNA|journal=Cell|date=June 1983|volume=33|issue=2|pages=320–322|url=http://www.cell.com/cell/pdf/0092-8674(83)90413-0.pdf|doi=10.1016/0092-8674(83)90413-0}} 6. ^{{cite journal|last1=Walker|first1=Thomas A.|last2=Pace|first2=Norman R.|title=5.8S Ribosomal RNA|journal=Cell|date=June 1983|volume=33|issue=2|pages=320–322|url=http://www.cell.com/cell/pdf/0092-8674(83)90413-0.pdf|doi=10.1016/0092-8674(83)90413-0}} 7. ^{{cite journal|title=An overview of pre-ribosomal RNA prcoessing in eukaryotes|journal=WIREs RNA|date=27 Oct 2014|volume=6|issue=2|pages=225–242|doi=10.1002/wrna.1269|pmid=25346433|pmc=4361047|last1=Henras|first1=A. K.|last2=Plisson-Chastang|first2=C.|last3=O'Donohue|first3=M. F.|last4=Chakraborty|first4=A.|last5=Gleizes|first5=P. E.}} 8. ^{{cite journal|title=An overview of pre-ribosomal RNA prcoessing in eukaryotes|journal=WIREs RNA|date=27 Oct 2014|volume=6|issue=2|pages=225–242|doi=10.1002/wrna.1269|pmid=25346433|pmc=4361047|last1=Henras|first1=A. K.|last2=Plisson-Chastang|first2=C.|last3=O'Donohue|first3=M. F.|last4=Chakraborty|first4=A.|last5=Gleizes|first5=P. E.}} External links
1 : Ribosomal RNA |
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