Klier is a surname. Notable people with the surname include:

• Andreas Klier (born 1976), German road racing cyclist
• Cornelia Klier (born 1957), German rower
• Freya Klier (born 1950), German author and film director
• Gerd Klier (1944–2011) German footballer
• John Klier (1944–2007), American historian
• Leo Klier (1923–2005), American basketball player
• Richard Klier (1891-?), Czech Olympic sports shooter

• Ki-2
• Ki-201
• Ki-30
• Ki-34
• Ki-38
• Ki-46
• Ki-48
• Ki-49
• Ki-57
• KI-574
• Ki-67 (disambiguation)
• Ki-86A
• KIAA0020 (gene)
• KIAA0101 (gene)
• KIAA0196 (gene)
• KIAA0319 (gene)
• KIAA0368 (gene)
• KIAA0430 (gene)
• KIAA0562
• KIAA0565
• KIAA0753
• Kiaa0753
• KIAA0802
• KIAA0895
• KIAA0895 (gene)