| Diplomatic accreditation | ambassador | Observations | President of Guatemala | List of popes | Term end | | 1851|06|23}} | Fernando Lorenzana|de}} | Chargé d'affaires | José Rafael Carrera Turcios | Pope Pius IX |
| 1936 | } | Francisco A. Figueroa | José María Reina Andrade | Pope Pius XII | 1949 | } |
| 1956 | } | Domingo Coicolea Villacorta | Carlos Castillo Armas | Pope Pius XII | 1958 | } |
| 1958 | } | Pedro De Yurrita Maury | Miguel Ydígoras Fuentes | Pope John XXIII | 1963 | } |
| 1963 | } | Luis Beltranena Sinibaldi | Alfredo Enrique Peralta Azurdia | Pope Paul VI | 1964 | } |
| 1964 | } | Eduardo de León Strecker | Chargé d'affaires | Alfredo Enrique Peralta Azurdia | Pope Paul VI | 1965 | } |
| 1965 | } | José Guirola Leal | Alfredo Enrique Peralta Azurdia | Pope Paul VI | 1966 | } |
| 1966 | } | Luis Valladares y Aycinena | Julio César Méndez Montenegro | Pope Paul VI | 1983 | } |
| 1983 | } | José Alejandro Deutschmann Miron | Óscar Humberto Mejía Víctores | Pope John Paul II | 1986 | } |
| 1986 | } | Julio Antonio Torres Arriola | Chargé d'affaires | Marco Vinicio Cerezo Arévalo | Pope John Paul II | 1987 | } |
| 1987 | } | Carlos Alfredo Escobar Armas | Marco Vinicio Cerezo Arévalo | Pope John Paul II | 1991 | } |
| 1991 | } | Carlos Urrutia-Aparicio | Jorge Antonio Serrano Elias | Pope John Paul II | 1992 | } |
| 1992 | } | Mario Alfonso de la Cerda Bustamente | Jorge Antonio Serrano Elias | Pope John Paul II | 1995 | } |
| 1995 | } | José Mauricio Rodríguez Wever | Ramiro de León Carpio | Pope John Paul II | 1998 | } |
| 1998 | } | Sergio Iván Búcaro Hurtarte | Álvaro Arzú Irigoyen | Pope John Paul II | 2000 | } |
| 2000 | } | Acisclo Valladares Molina | Alfonso Antonio Portillo Cabrera | Pope John Paul II | 2004 | } |
| 2004 | } | Juan Gavarrete Soberón | Óscar Berger Perdomo | Pope John Paul II | 2008 | } |
| 2008 | } | Acisclo Valladares Molina | Álvaro Colom Caballeros | Pope Benedict XVI | 2009 | } |
| 2009 | } | Martha Thelma Calderón Vandenberg | Chargé d'affaires | Álvaro Colom Caballeros | Pope Benedict XVI | 2010 | } |
| 2010|02|06}} | Alfonso Roberto Matta Fahsen | Álvaro Colom Caballeros | Pope Benedict XVI | 2016 | } |
| 2016|12|01}} | Alfredo Vásquez Rivera|de}} | Jimmy Morales | Pope Francis |
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