| 词条 | Mitochondrial calcium uniporter |
| 释义 |
| Symbol = MCU | Name = Mitochondrial calcium uniporter | image = | width = | caption = | InterPro= IPR018782 | SMART= | PROSITE = | SCOP = | TCDB = 1.A.77 | OPM family= 486 | OPM protein= 6dnf | Pfam= PF04678 | PDB= | Membranome superfamily= 216 }}{{Infobox protein | name = Mitochondrial calcium uniporter | image = | width = | caption = | Symbol = MCU | AltSymbols = C10orf42, CCDC109A, FLJ46135 | IUPHAR_id = | ATC_prefix = | ATC_suffix = | ATC_supplemental = | CAS_number = | CAS_supplemental = | DrugBank = | EntrezGene = 90550 | HGNCid = 23526 | OMIM = 614197 | PDB = Q8NE86 | RefSeq = NM_138357 | UniProt = Q8NE86 | ECnumber = | Chromosome = 10 | Arm = 22 | Band = 2 | LocusSupplementaryData = }} The mitochondrial calcium uniporter (MCU) is a transmembrane protein that allows the passage of calcium ions from a cell's cytosol into mitochondria.[1] Its activity is regulated by MICU1 and MICU2, which together with the MCU make up the mitochondrial calcium uniporter complex.[2] The MCU is one of the primary sources of mitochondria uptake of calcium, and flow is dependent on membrane potential of the inner mitochondrial membrane and the concentration of calcium in the cytosol relative to the concentration in the mitochondria. Balancing calcium concentration is necessary to increase the cell's energy supply and regulate cell death. Calcium is balanced through the MCU in conjunction with the sodium-calcium exchanger.[1] The MCU has a very low affinity for calcium, so the cytosolic calcium concentration needs to be approximately 5-10 uM for significant transport of calcium into the mitochondria. Mitochondria are closely associated with the endoplasmic reticulum (ER), at contact sites, which contains stores of cellular calcium ions for calcium signaling. The presence of 1,4,5-triphosphate (IP3) triggers the release of calcium from these intracellular stores, which creates microdomains of high calcium concentration between the ER and the mitochondria, creating the conditions for the MCU to take up calcium.[3] Ruthenium red and Ru360 are typical reagents used to experimentally block the MCU to study its properties and role in mitochondrial signaling.[4][5]References1. ^1 {{Cite web|title = Mitochondrial Calcium Uniporter|url = https://www.tocris.com/pharmacologicalBrowser.php?ItemId=355639&Information=True#.Vs3Yq1MrJR4|website = Tocris.com|access-date = 2016-02-24|publisher = Tocris Bioscience|date = 2016}} 2. ^{{Cite web|title = MCU - Calcium uniporter protein, mitochondrial precursor - Homo sapiens (Human)|url = https://www.uniprot.org/uniprot/Q8NE86|website = UniProt.org|access-date = 2016-02-24|publisher = UniProt Consortium}} 3. ^{{cite journal | vauthors = Marchi S, Pinton P | title = The mitochondrial calcium uniporter complex: molecular components, structure and physiopathological implications | journal = The Journal of Physiology | volume = 592 | issue = 5 | pages = 829–39 | date = March 2014 | pmid = 24366263 | pmc = 3948548 | doi = 10.1113/jphysiol.2013.268235 }} 4. ^{{cite journal | vauthors = Broekemeier KM, Krebsbach RJ, Pfeiffer DR | title = Inhibition of the mitochondrial Ca2+ uniporter by pure and impure ruthenium red | journal = Molecular and Cellular Biochemistry | volume = 139 | issue = 1 | pages = 33–40 | date = October 1994 | pmid = 7531818 | doi = 10.1007/bf00944201 }} 5. ^{{cite journal | vauthors = Matlib MA, Zhou Z, Knight S, Ahmed S, Choi KM, Krause-Bauer J, Phillips R, Altschuld R, Katsube Y, Sperelakis N, Bers DM | title = Oxygen-bridged dinuclear ruthenium amine complex specifically inhibits Ca2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes | journal = The Journal of Biological Chemistry | volume = 273 | issue = 17 | pages = 10223–31 | date = April 1998 | pmid = 9553073 | doi = 10.1074/jbc.273.17.10223 }} 3 : Transmembrane transporters|Calcium signaling|Mitochondria |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。