- Purpose
- Cell Lines
- Misidentification and Misclassification Continued Use of Wrong Classification
- List of NCI-60 Cell Lines
- List of Additional Cell Lines
- References
The NCI-60 cancer cell line panel is a group of 60 human cancer cell lines used by the National Cancer Institute (NCI) for the screening of compounds to detect potential anticancer activity.[1] Purpose The screening procedure is called the NCI-60 Human Tumor Cell Lines Screen, and it is one of the Discovery & Development Services of NCI's Developmental Therapeutics Program (DTP).[2][3][4] The screening rates for each cell line the cytostatic and cytotoxic impact of tested substances.[2] Due to the diversity of the cell lines, it is possible to compare tested compounds by their effect patterns, high correlation potentially corresponding to similar effect mechanisms.[2] An automated comparison against a database of more than 88,000 pure compounds and more than 34,000 crude extracts ({{as of|2017|01|06|lc=y}}) is provided by the COMPARE tool, which shows a list of substances ranked by the Pearson correlation coefficients for a given test substance.[5] The same panel is used in the Molecular Target Program for the characterization of molecular targets. Measurements include protein levels, RNA measurements, mutation status and enzyme activity levels.[6] Cell Lines The panel holds cell lines representing leukemia, melanoma, non-small-cell lung carcinoma, and cancers of the brain, ovary, breast, colon, kidney, and prostate.[1][2] 13 additional cell lines are evaluated for use in the screening program, among them two lines deriving from so far not represented small-cell lung carcinoma.[1] All cell lines but one ({{as of|2017|01|06|lc=y}}) are available to other laboratories, including the additional cell lines.[1] Misidentification and Misclassification Starting around the turn of the millennium, several cell lines were found to be misidentified or misclassified at the time of investigation or earlier:[7][8][9] MDA-MB-435, originally classified as breast cancer cell line, was identified as being a melanoma cell line.[7]When investigated further, current MDA-MB-435 appeared to be derived from the same individual as cell line M14.[7] An examination of clones made clear that the mixup with M14 happened very early in the history of MDA-MB-435, before establishment in the Developmental Therapeutics Program.[7] NCI/ADR-RES, originally classified as breast cancer cell line, was identified as being an ovarian tumor cell line.[8]NCI/ADR-RES appears to have been derived at some point in time from cell line OVCAR-8.[8] Originally the cell line was named MCF-7/ADR-RES; it was renamed together with the change in classification.[8] Two brain cancer cell lines, SNB-19 and U251, were discovered to come from the same person.[9] This makes a mixup likely. A 61th cell line, MDA-N, has been confirmed to being derived from the misclassified MDA-MB-435 cell line.[7] So it is also misclassified, really being a melanoma cell line, but {{as of|2018|01|06|lc=y}} the official website still lists it as breast cancer cell line.[1] This cell line is not available {{as of|2018|01|06|lc=y}}.[1] The International Cell Line Authentication Committee maintaines a list of contaminated cell lines.[10] It includes the cell lines reported above, although usually with reporting dates and articles considerably later than the first dates and articles rising specific concern regarding the respective cell line.[10][7][8][9] Continued Use of Wrong Classification {{as of|2014}} Hundreds of research studies still use MDA-MB-435 as model of breast cancer, even after it was officially declared to be a melanoma cell line in 2007, and even in highest-rated international peer-reviewed journals.[11]Similar is true for other misidentified cell lines.[11] List of NCI-60 Cell Lines The NCI-60 cell lines used by NCI's Human Tumor Cell Lines Screen as described on the website of the Developmental Therapeutics Program.[1][12] Links to corresponding Cellosaurus descriptions and Research Resource Identifiers (RRID) have been added.[12] The Cellosaurus identifier is a combination of the cell line name variant chosen by Cellosaurus, with the Cellosaurus accession ID, the latter written as RRID. | Cell line | Tumor classification | (Potentially) misidentified | Original classification | Unavailable | Doubling Time (Hours) | Inoculation Density | Cellosaurus identifier and link |
|---|
| CCRF-CEM | Leukemia | 26.7 | 40000 | [https://web.expasy.org/cellosaurus/CVCL_0207 CCRF-CEM (RRID:CVCL_0207)] | | HL-60(TB) | Leukemia | 28.6 | 40000 | [https://web.expasy.org/cellosaurus/CVCL_A794 HL-60(TB) (RRID:CVCL_A794)] | | K-562 | Leukemia | 19.6 | 5000 | [https://web.expasy.org/cellosaurus/CVCL_0004 K-562 (RRID:CVCL_0004)] | | MOLT-4 | Leukemia | 27.9 | 30000 | [https://web.expasy.org/cellosaurus/CVCL_0013 MOLT-4 (RRID:CVCL_0013)] | | RPMI-8226 | Leukemia | 33.5 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0014 RPMI-8226 (RRID:CVCL_0014)] | | SR | Leukemia | 28.7 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1711 SR (RRID:CVCL_1711)] | | A549/ATCC | Non-Small Cell Lung | 22.9 | 7500 | [https://web.expasy.org/cellosaurus/CVCL_0023 A-549 (RRID:CVCL_0023)] | | EKVX | Non-Small Cell Lung | 43.6 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1195 EKVX (RRID:CVCL_1195)] | | HOP-62 | Non-Small Cell Lung | 39 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1285 HOP-62 (RRID:CVCL_1285)] | | HOP-92 | Non-Small Cell Lung | 79.5 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1286 HOP-92 (RRID:CVCL_1286)] | | NCI-H226 | Non-Small Cell Lung | 61 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1544 NCI-H226 (RRID:CVCL_1544)] | | NCI-H23 | Non-Small Cell Lung | 33.4 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1547 NCI-H23 (RRID:CVCL_1547)] | | NCI-H322M | Non-Small Cell Lung | 35.3 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1557 NCI-H322M (RRID:CVCL_1557)] | | NCI-H460 | Non-Small Cell Lung | 17.8 | 7500 | [https://web.expasy.org/cellosaurus/CVCL_0459 NCI-H460 (RRID:CVCL_0459)] | | NCI-H522 | Non-Small Cell Lung | 38.2 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1567 NCI-H522 (RRID:CVCL_1567)] | | COLO 205 | Colon | 23.8 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_0218 COLO 205 (RRID:CVCL_0218)] | | HCC-2998 | Colon | 31.5 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1266 HCC2998 (RRID:CVCL_1266)] | | HCT116 | Colon | 17.4 | 5000 | [https://web.expasy.org/cellosaurus/CVCL_0291 HCT 116 (RRID:CVCL_0291)] | | HCT-15 | Colon | 20.6 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0292 HCT 15 (RRID:CVCL_0292)] | | HT-29 | Colon | 19.5 | 5000 | [https://web.expasy.org/cellosaurus/CVCL_0320 HT-29 (RRID:CVCL_0320)] | | KM12 | Colon | 23.7 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1331 KM12 (RRID:CVCL_1331)] | | SW-620 | Colon | 20.4 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0547 SW620 (RRID:CVCL_0547)] | | SF-268 | CNS | 33.1 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1689 SF268 (RRID:CVCL_1689)] | | SF-295 | CNS | 29.5 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1690 SF295 (RRID:CVCL_1690)] | | SF-539 | CNS | 35.4 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1691 SF539 (RRID:CVCL_1691)] | | SNB-19 | CNS | yes | CNS | 34.6 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_0535 SNB-19 (RRID:CVCL_0535)] | | SNB-75 | CNS | 62.8 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1706 SNB-75 (RRID:CVCL_1706)] | | U251 | CNS | yes | CNS | 23.8 | 7500 | [https://web.expasy.org/cellosaurus/CVCL_0021 U-251MG (RRID:CVCL_0021)] | | LOX IMVI | Melanoma | 20.5 | 7500 | [https://web.expasy.org/cellosaurus/CVCL_1381 LOX-IMVI (RRID:CVCL_1381)] | | MALME-3M | Melanoma | 46.2 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1438 Malme-3M (RRID:CVCL_1438)] | | M14 | Melanoma | 26.3 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1395 M14 (RRID:CVCL_1395)] | | MDA-MB-435 | Melanoma | yes | Breast | 25.8 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_0417 MDA-MB-435 (RRID:CVCL_0417)] | | SK-MEL-2 | Melanoma | 45.5 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0069 SK-MEL-2 (RRID:CVCL_0069)] | | SK-MEL-28 | Melanoma | 35.1 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0526 SK-MEL-28 (RRID:CVCL_0526)] | | SK-MEL-5 | Melanoma | 25.2 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0527 SK-MEL-5 (RRID:CVCL_0527)] | | UACC-257 | Melanoma | 38.5 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1779 UACC-257 (RRID:CVCL_1779)] | | UACC-62 | Melanoma | 31.3 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1780 UACC-62 (RRID:CVCL_1780)] | | IGR-OV1 | Ovarian | 31 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1304 IGROV-1 (RRID:CVCL_1304)] | | OVCAR-3 | Ovarian | 34.7 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0465 OVCAR-3 (RRID:CVCL_0465)] | | OVCAR-4 | Ovarian | 41.4 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1627 OVCAR-4] | | OVCAR-5 | Ovarian | 48.8 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1628 OVCAR-5 (RRID:CVCL_1628)] | | OVCAR-8 | Ovarian | 26.1 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1629 OVCAR-8 (RRID:CVCL_1629)] | | MCF-7/ADR-RES}}NCI/ADR-RES (originally MCF-7/ADR-RES) | Ovarian | yes | Breast | 34 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1452 NCI-ADR-RES (RRID:CVCL_1452)] | | SK-OV-3 | Ovarian | 48.7 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0532 SK-OV-3 (RRID:CVCL_0532)] | | 786-O | Renal | 22.4 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1051 786-O (RRID:CVCL_1051)] | | A498 | Renal | 66.8 | 25000 | [https://web.expasy.org/cellosaurus/CVCL_1056 A-498 (RRID:CVCL_1056)] | | ACHN | Renal | 27.5 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_1067 ACHN (RRID:CVCL_1067)] | | CAKI-1 | Renal | 39 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0234 Caki-1 (RRID:CVCL_0234)] | | RXF 393 | Renal | 62.9 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1673 RXF 393L (RRID:CVCL_1673)] | | SN12C | Renal | 29.5 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1705 SN12C (RRID:CVCL_1705)] | | TK-10 | Renal | 51.3 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1773 TK-10 (RRID:CVCL_1773)] | | UO-31 | Renal | 41.7 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1911 UO-31 (RRID:CVCL_1911)] | | PC-3 | Prostate | 27.1 | 7500 | [https://web.expasy.org/cellosaurus/CVCL_0035 PC-3 (RRID:CVCL_0035)] | | DU-145 | Prostate | 32.3 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0105 DU145 (RRID:CVCL_0105)] | | MCF7 | Breast | 25.4 | 10000 | [https://web.expasy.org/cellosaurus/CVCL_0031 MCF-7 (RRID:CVCL_0031)] | | MDA-MB-231/ATCC | Breast | 41.9 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0062 MDA-MB-231 (RRID:CVCL_0062)] | | MDA-MB-468 | Breast | 62 | 2000 | [https://web.expasy.org/cellosaurus/CVCL_0419 MDA-MB-468 (RRID:CVCL_0419)] | | HS 578T | Breast | 53.8 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0332 Hs 578T (RRID:CVCL_0332)] | | MDA-N | Melanoma | yes | Breast | unavailable | 22.5 | 15000 | [https://web.expasy.org/cellosaurus/CVCL_1910 MDA-N (RRID:CVCL_1910)] | | BT-549 | Breast | 53.9 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1092 BT-549 (RRID:CVCL_1092)] | | T-47D | Breast | 45.5 | 20000 | [https://web.expasy.org/cellosaurus/CVCL_0553 T-47D (RRID:CVCL_0553)] |
List of Additional Cell Lines Additional cell lines evaluated for use in NCI's Tumor Cell Lines Screen as described on the website of the Developmental Therapeutics Program.[1] Links to corresponding Cellosaurus descriptions and Research Resource Identifiers (RRID) have been added, as well as the species.[13] | Cell line | Tumor classification | Species | Inoculation Density | Cellosaurus identifier and link |
|---|
| LXFL 529 | Non-Small Cell Lung | human | 10000 | [https://web.expasy.org/cellosaurus/CVCL_D085 LXFL 529L (RRID:CVCL_D085)] | | DMS 114 | Small Cell Lung | human | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1174 DMS 114 (RRID:CVCL_1174)] | | SHP-77 | Small Cell Lung | human | 40000 | [https://web.expasy.org/cellosaurus/CVCL_1693 SHP-77 (RRID:CVCL_1693)] | | DLD-1 | Colon | human | 50000 | [https://web.expasy.org/cellosaurus/CVCL_0248 DLD-1 (RRID:CVCL_0248)] | | KM20L2 | Colon | human | 10000 | [https://web.expasy.org/cellosaurus/CVCL_D889 KM20L2 (RRID:CVCL_D889)] | | SNB-78 | CNS | human | 20000 | [https://web.expasy.org/cellosaurus/CVCL_B321 SNB-78 (RRID:CVCL_B321)] | | XF 498 | CNS | human | 20000 | [https://web.expasy.org/cellosaurus/CVCL_8928 XF 498 (RRID:CVCL_8928)] | | RPMI-7951 | Melanoma | human | 20000 | [https://web.expasy.org/cellosaurus/CVCL_1666 RPMI-7951 (RRID:CVCL_1666)] | | M19-MEL | Melanoma | human | 10000 | [https://web.expasy.org/cellosaurus/CVCL_B415 M19-MEL (RRID:CVCL_B415)] | | RXF-631 | Renal | human | 10000 | [https://web.expasy.org/cellosaurus/CVCL_A780 RXF 631L (RRID:CVCL_A780)] | | SN12K1 | Renal | human | 10000 | [https://web.expasy.org/cellosaurus/CVCL_D106 SN12K1 (RRID:CVCL_D106)] | | P388 | Leukemia | mouse | 50000 | [https://web.expasy.org/cellosaurus/CVCL_7222 P388 (RRID:CVCL_7222)] | | P388/ADR | Leukemia | mouse | 50000 | [https://web.expasy.org/cellosaurus/CVCL_IZ75 P388/ADR (RRID:CVCL_IZ75)] |
References 1. ^1 2 3 4 5 6 7 {{cite web |url= https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm |title= Cell Lines in the In Vitro Screen |date= 8 May 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
2. ^1 2 3 {{cite web |url= https://dtp.cancer.gov/discovery_development/nci-60/default.htm |title= NCI-60 Human Tumor Cell Lines Screen |date= 26 August 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
3. ^{{cite web |url= https://dtp.cancer.gov/discovery_development/default.htm |title= Discovery & Development Services |date= 26 August 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
4. ^{{cite web |url= https://dtp.cancer.gov/ |title= Welcome to the Developmental Therapeutics Program |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
5. ^{{cite web |url= https://dtp.cancer.gov/databases_tools/compare.htm |title= COMPARE Analysis |date= 11 August 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
6. ^{{cite web |url= https://dtp.cancer.gov/databases_tools/molecular_target/default.htm |title= Molecular Targets |date= 12 May 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 7 January 2018}}
7. ^1 2 3 4 5 {{cite web |url= https://dtp.cancer.gov/discovery_development/nci-60/mda-mb-435.htm |title= MDA-MB-435, and its derivation MDA-N, are Melanoma cell lines, not breast cancer cell lines. |date= 8 May 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
8. ^1 2 3 4 {{cite web |url= https://dtp.cancer.gov/discovery_development/nci-60/nci-adrres.htm |title= Cell Line NCI/ADR-RES is an Ovarian tumor cell line, not a Breast line. |date= 8 May 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
9. ^1 2 {{cite web |url= https://dtp.cancer.gov/discovery_development/nci-60/u251_snb-19.htm |title= CNS cell lines SNB-19 and U251 are derived from the same individual. |date= 8 May 2015 |website= Developmental Therapeutics Program |publisher= National Cancer Institute |access-date= 6 January 2018}}
10. ^1 {{Citation| date = 1 December 2016| title = Database of Cross-Contaminated or Misidentified Cell Lines| publisher = International Cell Line Authentication Committee (ICLAC)| url = http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf| version = 8.0| accessdate = 6 January 2018| format = PDF}}
11. ^1 {{cite journal| last = Prasad| first = Vidudala VTS| last2 = Gopalan| first2 = Ramprasad OG| date = 2 June 2015| title = Continued use of MDA-MB-435, a melanoma cell line, as a model for human breast cancer, even in year, 2014| url = http://www.nature.com/articles/npjbcancer20152| journal = Npj Breast Cancer| volume = 1| pages = 15002| doi = 10.1038/npjbcancer.2015.2| access-date = 6 January 2018}}
12. ^1 {{cite web |url= https://web.expasy.org/cgi-bin/cellosaurus/search?input=%22NCI60%20cancer%20cell%20line%20panel%22 |title= Cellosaurus search result: 61 hits for "NCI60 cancer cell line panel" |website= Cellosaurus |publisher= Swiss Institute of Bioinformatics |access-date= 7 January 2018}}The reference includes the list of linked pages in the search result.
13. ^{{cite web |url= https://web.expasy.org/cellosaurus/ |title= Cellosaurus - a knowledge resource on cell lines |website= Cellosaurus |publisher= Swiss Institute of Bioinformatics |access-date= 17 January 2018}}The reference includes the pages found by searching with the respective cell line names as given in the List of Additional Cell Lines.
2 : Human cell lines|Ovarian cancer |