“NE-tag”的意思、由来-开放百科全书

The NE-tag is a synthetic peptide tag (NE tag) designed as an epitope tag for detection, quantification and purification of recombinant protein. This patented peptide sequence is composed of eighteen hydrophilic amino acids. This short peptide does not adopt any significant homology to any existing proteins found in nature. This synthetic NE peptide adopts random coil conformation and showing strong immunogenicity (computational prediction). This is advantageous to offer stringent specificity to the NE-tagged proteins, which are readily to be detected, quantitated, and purified.^[1]

Detection

The NE-tag can be specifically detected using a monoclonal anti-NE detection antibody - an affinity-purified mouse immunoglobulin, IgG1, which specifically binds to NE-tagged proteins. This peptide-to-antibody conjugation is validated in Western blotting, immunoprecipitation (IP), immunocytochemistry (IHC), and affinity purification of NE fusion proteins (i.e. affinity column).^[2]^[3]

Sequences

Patents

References

1. ^{{cite journal |last1=Ho |first1=Philip WL. |last2=Tse |first2=Zero HM. |last3=Liu |first3=HF. |last4=Lu |first4=S. |last5=Ho |first5=Jessica WM. |last6=Kung |first6=Michelle HW. |last7=Ramsden |first7=David B. |last8=Ho |first8=SL. |year=2013 |title= Assessment of cellular estrogenic activity based on estrogen receptor-mediated reduction of soluble-form catechol-O-methyltransferase (COMT) expression in an ELISA-based system. |journal=PLoS ONE |volume=8 |issue=9 |pages=e74065 |pmid=24040167 |doi=10.1371/journal.pone.0074065 |pmc=3765251}}
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3. ^{{cite journal |last1=Xu |first1=EG. |last2=Ho |first2=Philip WL. |last3=Tse |first3=Zero HM. |last4=Ho |first4=SL. |last5=Leung |first5=KM. |year=2016 |title=Revealing ecological risks of priority endocrine disrupting chemicals in four marine protected areas in Hong Kong through an integrative approach. |journal=Environmental Pollution |volume=215 |pages=103–112 |pmid=27179329 |doi=10.1016/j.envpol.2016.04.090}}