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词条 Allotopic expression
释义

  1. Use in Therapy

  2. Research

  3. References

{{Short description|type of gene expression}}{{Use dmy dates|date=November 2016}}Allotopic expression (AE) refers to expression of genes in the cell nucleus that normally are expressed only from the mitochondrial genome. Biomedically engineered AE has been suggested as a possible future tool in gene therapy of certain mitochondria-related diseases,[1] however this view is controversial.[2] While this type of expression has been successfully carried out in yeast, the results in mammals have been conflicting.[3]

Use in Therapy

Gensight Biologics are currently pursuing a clinical program of expressing the ND4 gene in the nucleus to prevent retinal disease.[4]

Research

The SENS Research Foundation recently reported success in expressing the ATP6 gene allotopically in vitro.[5]

As of 6 September 2016 and as a result of funds raised at lifespan.io SENS Research Foundation showed ATP6 and ATP8 could be successfully expressed and their published research appears in Nucleic Acids Research [6] providing proof of concept for the MitoSENS repair approach for repairing age related damage.

References

1. ^{{cite journal |author =de Grey AD |title=Mitochondrial gene therapy: an arena for the biomedical use of inteins |journal=Trends Biotechnol. |volume=18 |issue=9 |pages=394–9 |date=September 2000 |pmid=10942964 |doi=10.1016/S0167-7799(00)01476-1 |url=http://linkinghub.elsevier.com/retrieve/pii/S0167-7799(00)01476-1}}
2. ^{{cite journal |vauthors=Oca-Cossio J, Kenyon L, Hao H, Moraes CT |title=Limitations of allotopic expression of mitochondrial genes in mammalian cells |journal=Genetics |volume=165 |issue=2 |pages=707–20 |date=October 2003 |pmid=14573482 |pmc=1462783 }}
3. ^{{cite journal | title=Allotopic expression of mitochondrial-encoded genes in mammals: achieved goal, undemonstrated mechanism or impossible task? | author=Perales-Clemente, Ester | journal=Nucleic Acids Res. |date=Jan 2011 | volume=39 | issue=1 | pages=225–234 | doi=10.1093/nar/gkq769 | pmid=20823090 | pmc=3017613|display-authors=etal}}
4. ^{{Cite web|url=http://www.gensight-biologics.com/index.php?page=mts|title=Gensight|website=www.gensight-biologics.com|access-date=2016-05-20}}
5. ^{{Cite web|url=https://www.lifespan.io/campaigns/sens-mitochondrial-repair-project/#updates|title=MitoSENS Project Results to be Published in the journal Nucleic Acids Research!|date=18 August 2016|website=|publisher=lifespan.io|accessdate=28 November 2016 }}
6. ^{{cite web | url=http://nar.oxfordjournals.org/content/early/2016/09/04/nar.gkw756 | title=Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant | publisher=Oxford Journals | work=Nucleic Acids Research | accessdate=28 November 2016 }}

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