| 词条 | Anti-Q RNA |
| 释义 |
| Name = Anti-Q RNA | image = RF00388.jpg | width = | caption = Predicted secondary structure and sequence conservation of Anti-Q RNA | Symbol = Anti-Q_RNA | AltSymbols = QaRNA | Rfam = RF00388 | miRBase = | miRBase_family = | RNA_type = Gene; antisense | Tax_domain = Bacteria | GO = | SO = {{SO|0000644}} | CAS_number = | EntrezGene = | HGNCid = | OMIM = | PDB = | RefSeq = | Chromosome = | Arm = | Band = | LocusSupplementaryData = }} Anti-Q RNA (formerly Qa RNA) is a small ncRNA from the conjugal plasmid pCF10 of Enterococcus faecalis. It is coded in cis to its regulatory target, prgQ, but can also act in trans. Anti-Q is known to interact with nascent prgQ transcripts to allow formation of an intrinsic terminator, or attenuator, thus preventing transcription of downstream genes.[1][2] This mode of regulation is essentially the same as that of the countertranscript-driven attenuators that control copy number in pT181,[3] pAMbeta1[4] and pIP501[5] and related Staphylococcal plasmids. Anti-Q is transcribed from the same segment of DNA as prgQ, except from the opposite strand, making it perfectly complementary to a portion of prgQ. Further experiments have experimentally confirmed the original consensus secondary structure and demonstrated that only certain regions of Anti-Q interact with prgQ.[6] Anti-Q is derived from the 5’ end a longer transcript. The 3’ end of this transcript encodes PrgX, a repressor of prgQ transcription. References1. ^{{cite journal | last = Bae | first = T |author2=Kozlowicz BK|author3=Dunny GM | year = 2004 | title = Characterization of cis-acting prgQ mutants: evidence for two distinct repression mechanisms by Qa RNA and PrgX protein in pheromone-inducible enterococcal plasmid pCF10 | journal = Mol Microbiol | volume = 51 | pages = 271–281 | pmid = 14651627 | doi = 10.1046/j.1365-2958.2003.03832.x | issue = 1}} 2. ^{{cite journal |vauthors=Johnson CM, Manias DA, Haemig HA, Shokeen S, Weaver KE, Henkin TM, Dunny GM |title=Direct evidence for control of the pheromone-inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript-driven attenuation mechanism |journal=J Bacteriol |volume=192 |issue=6 |pages=1634–42 |year=2010 |pmid=20097859 |doi=10.1128/JB.01525-09 |pmc=2832536}} 3. ^{{cite journal |vauthors=Novick RP, Iordanescu S, Projan SJ, Kornblum J, Edelman I |title=pT181 plasmid replication is regulated by a countertranscript-driven transcriptional attenuator |journal=Cell |volume=59 |issue=2 |pages=395–404 |year=1989 |pmid=2478296 |doi=10.1016/0092-8674(89)90300-0}} 4. ^{{cite journal |vauthors=Le Chatelier E, Ehrlich SD, Jannière L |title=Countertranscript-driven attenuation system of the pAM beta 1 repE gene |journal=Mol Microbiol |volume=20 |issue=5 |pages=1099–112 |year=1996 |pmid=8809762 |doi=10.1111/j.1365-2958.1996.tb02550.x}} 5. ^{{cite journal |vauthors=Brantl S, Birch-Hirschfeld E, Behnke D |title=RepR protein expression on plasmid pIP501 is controlled by an antisense RNA-mediated transcription attenuation mechanism |journal=J Bacteriol |volume=175 |issue=13 |pages=4052–61 |year=1993 |pmid=8320221 |pmc=204834}} 6. ^{{cite journal |vauthors=Shokeen S, Johnson CM, Greenfield TJ, Manias DA, Dunny GM, E Weaver K |title=Structural analysis of the Anti-Q-Qs interaction: RNA-mediated regulation of E. faecalis plasmid pCF10 conjugation |journal=Plasmid |volume= 64|issue= 1|pages= 26–35|year=2010 |pmid=20332003 |doi=10.1016/j.plasmid.2010.03.002 |pmc=2892192}} External links
1 : Non-coding RNA |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。