| 词条 | Burkholderia pseudomallei |
| 释义 |
| image = Bps close.JPG | image_width = 240px | image_caption = B. pseudomallei colonies on Ashdown's agar showing the characteristic cornflower head morphology | regnum = Bacteria | phylum = Proteobacteria | classis = Betaproteobacteria | ordo = Burkholderiales | familia = Burkholderiaceae | genus = Burkholderia | species = B. pseudomallei | binomial = Burkholderia pseudomallei | binomial_authority = (Whitmore 1913) Yabuuchi et al. 1993[1] | synonyms = Bacillus pseudomallei Whitmore 1913 Bacterium whitmori Stanton and Fletcher 1921 Malleomyces pseudomallei Breed 1939 Loefflerella pseudomallei Brindle and Cowan 1951 Pfeiferella pseudomallei Pseudomonas pseudomallei (Whitmore 1913) Haynes 1957}}Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium.[2] It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia.[3] It infects humans and animals and causes the disease melioidosis. It is also capable of infecting plants.[4] B. pseudomallei measures 2–5 μm in length and 0.4–0.8 μm in diameter and is capable of self-propulsion using flagella. The bacteria can grow in a number of artificial nutrient environments, especially betaine- and arginine-containing ones. In vitro, optimal proliferation temperature is reported around 40 °C in neutral or slightly acidic environments (pH 6.8–7.0). The majority of strains are capable of fermentation of sugars without gas formation (most importantly, glucose and galactose; older cultures are reported to also metabolize maltose and starch). Bacteria produce both exo- and endotoxins. The role of the toxins identified in the process of melioidosis symptom development has not been fully elucidated.[5]IdentificationB. pseudomallei is not fastidious and grows on a large variety of culture media (blood agar, MacConkey agar, EMB, etc.). Ashdown's medium (or Burkholderia cepacia medium) may be used for selective isolation.[6]Cultures typically become positive in 24 to 48 hours (this rapid growth rate differentiates the organism from B. mallei, which typically takes a minimum of 72 hours to grow). Colonies are wrinkled, have a metallic appearance, and possess an earthy odour. On Gram staining, the organism is a Gram-negative rod with a characteristic "safety pin" appearance (bipolar staining). On sensitivity testing, the organism appears highly resistant (it is innately resistant to a large number of antibiotics including colistin and gentamicin) and that again differentiates it from B. mallei, which is in contrast, exquisitely sensitive to a large number of antibiotics. For environmental specimens only, differentiation from the nonpathogenic B. thailandensis using an arabinose test is necessary (B. thailandensis is never isolated from clinical specimens).[7] The laboratory identification of B. pseudomallei has been described in the literature.[8] The classic textbook description of B. pseudomallei in clinical samples is of an intracellular, bipolar-staining, Gram-negative rod, but this is of little value in identifying the organism from clinical samples.[8] Some[9] suggest the Wayson stain is useful for this purpose, but this has been shown not to be the case.[10] Laboratory identification of B. pseudomallei can be difficult, especially in Western countries where it is rarely seen. The large, wrinkled colonies look like environmental contaminants, so are often discarded as being of no clinical significance. Colony morphology is very variable and a single strain may display multiple colony types,[11][12] so inexperienced laboratory staff may mistakenly believe the growth is not pure. The organism grows more slowly than other bacteria that may be present in clinical specimens, and in specimens from nonsterile sites, is easily overgrown. Nonsterile specimens should, therefore, be cultured in selective media (e.g., Ashdown's[13][14] or B. cepacia medium).[6] For heavily contaminated samples, such as faeces, a modified version of Ashdown's that includes norfloxacin, amoxicillin, and polymyxin B has been proposed.[15] In blood culture, the BacT/ALERT MB system (normally used for culturing mycobacteria) by bioMérieux has been shown to have superior yields compared to conventional blood culture media.[16] Even when the isolate is recognised to be significant, commonly used identification systems may misidentify the organism as Chromobacterium violaceum or other nonfermenting, Gram-negative bacilli such as Burkholderia cepacia or Pseudomonas aeruginosa.[17][18] Again, because the disease is rarely seen in Western countries, identification of B. pseudomallei in cultures may not actually trigger alarms in physicians unfamiliar with the disease.[19] Routine biochemical methods for identification of bacteria vary widely in their identification of this organism: the API 20NE system accurately identifies B. pseudomallei in 99% of cases,[20] as does the automated Vitek 1 system, but the automated Vitek 2 system only identifies 19% of isolates.[18] The pattern of resistance to antimicrobials is distinctive, and helps to differentiate the organism from P. aeruginosa. The majority of B. pseudomallei isolates are intrinsically resistant to all aminoglycosides (via an efflux pump mechanism),[21] but sensitive to co-amoxiclav:[22] this pattern of resistance almost never occurs in P. aeruginosa and is helpful in identification.[23] Unfortunately, the majority of strains in Sarawak, Borneo, are susceptible to aminoglycosides and macrolides, which means the conventional recommendations for isolation and identification do not apply there.[24] Molecular methods (PCR) of diagnosis are possible, but not routinely available for clinical diagnosis.[25][26] Fluorescence in situ hybridisation has also been described, but has not been clinically validated, and it is not commercially available.[27] In Thailand, a latex agglutination assay is widely used,[20] while a rapid immunofluorescence technique is also available in a small number of centres.[28] DisinfectionB. pseudomallei is susceptible to numerous disinfectants, including benzalkonium chloride, iodine, mercuric chloride, potassium permanganate, 1% sodium hypochlorite, 70% ethanol, 2% glutaraldehyde, and to a lesser extent, phenolic preparations.[29] B. pseudomallei is effectively killed by the commercial disinfectants, Perasafe and Virkon.[30] The microorganism can also be destroyed by heating to above 74 °C for 10 min or by ultraviolet irradiation.[31] B. pseudomallei is not reliably disinfected by chlorine.[32][33]Medical importance{{Main|Melioidosis}}B. pseudomallei infection in humans is called melioidosis; its mortality is 20 to 50% even with treatment.[22]Antibiotic treatment and sensitivity testing{{main|Melioidosis#Treatment|l1=Melioidosis treatment}}The antibiotic of choice is ceftazidime.[22] While various antibiotics are active in vitro (e.g., chloramphenicol, doxycycline, co-trimoxazole), they have been proven to be inferior in vivo for the treatment of acute melioidosis.[34] Disc diffusion tests are unreliable when looking for co-trimoxazole resistance in B. pseudomallei (they greatly overestimate resistance) and Etests or agar dilution tests should be used in preference.[35][36] The actions of co-trimoxazole and doxycycline are antagonistic, which suggests these two drugs ought not to be used together.[37] The organism is intrinsically resistant to gentamicin[38] and colistin, and this fact is helpful in the identification of the organism.[39] Kanamycin is used to kill B. pseudomallei in the laboratory, but the concentrations used are much higher than those achievable in humans.[40] Pathogenicity mechanisms and virulence factorsB. pseudomallei is an opportunistic pathogen. An environmental organism, it has no requirement to pass through an animal host to replicate. From the point of view of the bacterium, human infection is a developmental "dead end".[41]Strains which cause disease in humans differ from those causing disease in other animals, by possessing certain genomic islands.[42] It may have the ability to cause disease in humans because of DNA it has acquired from other microorganisms.[42] Its mutation rate is also high, and the organism continues to evolve even after infecting a host.[43] B. pseudomallei is able to invade cells (it is an intracellular pathogen).[44] It is able to polymerise actin, and to spread from cell to cell, causing cell fusion and the formation of multinucleated giant cells.[45] It possesses a uniquely fusogenic type VI secretion system that is required for cell-cell spread and virulence in mammalian hosts.[46] The bacterium also expresses a toxin called lethal factor 1.[47] B. pseudomallei is one of the first Proteobacteria to be identified as containing an active type VI secretion system. It is also the only organism identified that contains up to six different type VI secretion systems.[48]B. pseudomallei is intrinsically resistant to a large number of antimicrobial agents by virtue of its efflux pump mechanism. This mediates resistance to aminoglycosides (AmrAB-OprA), tetracyclines, fluoroquinolones, and macrolides (BpeAB-OprB).[49]Vaccine candidatesNo vaccine is currently available, but a number of vaccine candidates have been suggested. Aspartate-β-semialdehyde dehydrogenase (asd) gene deletion mutants are auxotrophic for diaminopimelate (DAP) in rich media and auxotrophic for DAP, lysine, methionine, and threonine in minimal media.[50] The Δasd bacterium (bacterium with the asd gene removed) protects against inhalational melioidosis in mice.[51] References1. ^{{cite journal|title=Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov.|journal=Microbiol Immunol|volume=36|pages=1251–75|year=1992|pmid=1283774|last1=Yabuuchi|first1=E|last2=Kosako|first2=Y|last3=Oyaizu|first3=H|last4=Yano|first4=I|last5=Hotta|first5=H|last6=Hashimoto|first6=Y|last7=Ezaki|first7=T|last8=Arakawa|first8=M|issue=12|doi=10.1111/j.1348-0421.1992.tb02129.x}} 2. ^{{cite web | title=Burkholderia pseudomallei | work=VirginiaTech Pathogen Database | url=http://pathport.vbi.vt.edu/pathinfo/pathogens/Burkholderia_pseudomallei.html | accessdate=2006-03-26 | deadurl=yes | archiveurl=https://web.archive.org/web/20060901171424/http://pathport.vbi.vt.edu/pathinfo/pathogens/Burkholderia_pseudomallei.html | archivedate=2006-09-01 | df= }} 3. ^{{Cite journal|last=Limmathurotsakul|first=Direk|last2=Golding|first2=Nick|last3=Dance|first3=David A. B.|last4=Messina|first4=Jane P.|last5=Pigott|first5=David M.|last6=Moyes|first6=Catherine L.|last7=Rolim|first7=Dionne B.|last8=Bertherat|first8=Eric|last9=Day|first9=Nicholas P. J.|date=2016-01-11|title=Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis|journal=Nature Microbiology|language=en|volume=1|issue=1|doi=10.1038/nmicrobiol.2015.8|issn=2058-5276|pmc=4746747|pmid=26877885|page=15008}} 4. ^{{cite journal|vauthors=Lee YH, Chen Y, Ouyang X, Gan YH |title=Identification of tomato plant as a novel host model for Burkholderia pseudomallei|journal=BMC Microbiol|year=2010|volume=10|pages=28|pmid=20109238|doi=10.1186/1471-2180-10-28|pmc=2823722}} 5. ^{{cite journal |vauthors=Haase A, Janzen J, Barrett S, Currie B |title=Toxin production by Burkholderia pseudomallei strains and correlation with severity of melioidosis |journal=Journal of Medical Microbiology |volume=46 |issue=7 |pages=557–63 |date=July 1997 |pmid=9236739 |doi= 10.1099/00222615-46-7-557}} 6. ^1 {{cite journal |vauthors=Peacock SJ, Chieng G, Cheng AC |title=Comparison of Ashdown's medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei |journal=Journal of Clinical Microbiology |volume=43 |issue=10 |pages=5359–61 |date=October 2005 |pmid=16208018 |pmc=1248505 |doi=10.1128/JCM.43.10.5359-5361.2005 |display-authors=etal}} 7. ^{{cite journal |vauthors=Chaiyaroj SC, Kotrnon K, Koonpaew S, Anantagool N, White NJ, Sirisinha S |title=Differences in genomic macrorestriction patterns of arabinose-positive (Burkholderia thailandensis) and arabinose-negative Burkholderia pseudomallei |journal=Microbiology and Immunology |volume=43 |issue=7 |pages=625–30 |year=1999 |pmid=10529102 |doi= 10.1111/j.1348-0421.1999.tb02449.x}} 8. ^1 {{cite journal| pmid = 9069100|vauthors=Walsh AL, Wuthiekanun V | title = The laboratory diagnosis of melioidosis.| journal = Br J Biomed Sci| year = 1996| volume = 53| issue = 4| pages = 249–53}} 9. ^{{cite journal |vauthors=Brundage WG, Thuss CJ, Walden DC |title=Four fatal cases of melioidosis in U. S. soldiers in Vietnam. Bacteriologic and pathologic characteristics |journal=The American Journal of Tropical Medicine and Hygiene |volume=17 |issue=2 |pages=183–91 |date=March 1968 |pmid=4869109 |doi= 10.4269/ajtmh.1968.17.183}} 10. ^{{cite journal |vauthors=Sheridan EA, Ramsay AR, Short JM, Stepniewska K, Wuthiekanun V, Simpson AJ |title=Evaluation of the Wayson stain for the rapid diagnosis of melioidosis |journal=Journal of Clinical Microbiology |volume=45 |issue=5 |pages=1669–70 |date=May 2007 |pmid=17360835 |pmc=1865910 |doi=10.1128/JCM.00396-07 }} 11. ^{{cite journal|vauthors=Chantratita N, Wuthiekanun V, Boonbumrung K | title = Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei.| journal = J Bacteriol| year= 2007| volume = 189| issue =3| pages = 807–17| pmid = 17114252| doi=10.1128/JB.01258-06| pmc=1797308|display-authors=etal}} 12. ^{{cite journal|vauthors=Pumpuang A, Chantratita N, Wikraiphat C | title = Survival of Burkholderia pseudomallei in distilled water for 16 years| journal = Trans R Soc Trop Med Hyg| year = 2011| volume = 105| issue = 10–2| pages = 598–600| doi = 10.1016/j.trstmh.2011.06.004 |pmid=21764093 |pmc=3183224 |url=http://trstmh.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=21764093|display-authors=etal}} 13. ^{{cite journal|author=Ashdown LR|title=An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens|journal=Pathology| year=1979|volume=11|issue=2|pages=293–7|pmid=460953|doi=10.3109/00313027909061954}} 14. ^{{cite journal|author1=Roesnita B |author2=Tay ST |author3=Puthucheary SD |author4=Sam IC. | title = Diagnostic use of Burkholderia pseudomallei selective media in a low prevalence setting.| journal = Trans R Soc Trop Med Hyg| year = 2012| volume = 106| issue = 2| pages = 131–3| pmid = 22112687| doi=10.1016/j.trstmh.2011.10.007}} 15. ^{{cite journal|vauthors=Goodyear A, Strange L, Rholl DA |title=An improved selective culture medium enhances the isolation of Burkholderia pseudomallei from contaminated specimens.|journal=Am J Trop Med Hyg|year=2013|volume=89|issue=5|pages=973–82|doi=10.4269/ajtmh.13-0119 |pmid=24062483 |pmc=3820346 |url=http://www.ajtmh.org/cgi/pmidlookup?view=long&pmid=24062483|display-authors=etal}} 16. ^{{cite journal|vauthors=Jorakate P, Higdon M, Kaewpan A |title=Contribution of the BacT/ALERT MB Mycobacteria Bottle to bloodstream infection surveillance in Thailand: added yield for Burkholderia pseudomallei.|journal=J Clin Microbiol|date=2015|pmid=25588650|doi=10.1128/JCM.02008-14|volume=53|issue=3|pages=910–4|display-authors=etal|pmc=4390673}} 17. ^{{cite journal |vauthors=Inglis TJ, Chiang D, Lee GS, Chor-Kiang L |title=Potential misidentification of Burkholderia pseudomallei by API 20NE |journal=Pathology |volume=30 |issue=1 |pages=62–4 |date=February 1998 |pmid=9534210 |doi=10.1080/00313029800169685 }} 18. ^1 {{cite journal |vauthors=Lowe P, Engler C, Norton R |title=Comparison of automated and nonautomated systems for identification of Burkholderia pseudomallei |journal=Journal of Clinical Microbiology |volume=40 |issue=12 |pages=4625–7 |date=December 2002 |pmid=12454163 |pmc=154629 |doi=10.1128/JCM.40.12.4625-4627.2002 }} 19. ^{{cite journal |vauthors=Kite-Powell A, Livengood JR, Suarez J |title=Imported Melioidosis—South Florida, 2005|journal=Morb Mortal Wkly Rep|year=2006|volume=55|issue=32|pages=873–6|pmid=16915220|url=https://www.cdc.gov/mmwr/preview/mmwrhtml/mm5532a1.htm|display-authors=etal}} 20. ^1 {{cite journal |vauthors=Amornchai P, Chierakul W, Wuthiekanun V |title=Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test |journal=Journal of Clinical Microbiology |volume=45 |issue=11 |pages=3774–6 |date=November 2007 |pmid=17804660 |pmc=2168515 |doi=10.1128/JCM.00935-07 |display-authors=etal}} 21. ^{{cite journal |vauthors=Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE |title=Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei |journal=Antimicrobial Agents and Chemotherapy |volume=43 |issue=3 |pages=465–70 |date=March 1999 |pmid=10049252 |pmc=89145 |doi= 10.1128/AAC.43.3.465|url=http://aac.asm.org/cgi/pmidlookup?view=long&pmid=10049252}} 22. ^1 2 {{cite journal |vauthors=Wuthiekanun V, Peacock SJ |title=Management of melioidosis |journal=Expert Review of Anti-infective Therapy |volume=4 |issue=3 |pages=445–55 |date=June 2006 |pmid=16771621 |doi=10.1586/14787210.4.3.445 }} 23. ^{{cite journal|vauthors=Hodgson K, Engler C, Govan B | title = A comparison of routine bench and molecular diagnostic methods in the identification of Burkholderia pseudomallei| journal = J Clin Microbiol| year = 2009| doi = 10.1128/JCM.02507-08| pmid = 19279182| volume = 47| issue = 5| pages = 1578–80| pmc = 2681847|display-authors=etal}} 24. ^{{cite journal|vauthors=Podin Y, Sarovich DS, Price EP, Kaestli M, Mayo M, Hii K | title = Burkholderia pseudomallei from Sarawak, Malaysian Borneo are predominantly susceptible to aminoglycosides and macrolides| journal = Antimicrob Agents Chemother| volume = 58| pages = 162–6| year = 2013| pmid = 24145517| doi = 10.1128/AAC.01842-13| issue=1|display-authors=etal | pmc=3910780}} 25. ^{{cite journal |vauthors=Ruppitsch W, Stöger A, Indra A |title=Suitability of partial 16S ribosomal RNA gene sequence analysis for the identification of dangerous bacterial pathogens |journal=Journal of Applied Microbiology |volume=102 |issue=3 |pages=852–9 |date=March 2007 |pmid=17309636 |doi=10.1111/j.1365-2672.2006.03107.x |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=1364-5072&date=2007&volume=102&issue=3&spage=852 |archive-url=https://archive.today/20120722075534/http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=1364-5072&date=2007&volume=102&issue=3&spage=852 |dead-url=yes |archive-date=2012-07-22 |display-authors=etal }} 26. ^{{cite journal |vauthors=Wattiau P, Van Hessche M, Neubauer H, Zachariah R, Wernery U, Imberechts H |title=Identification of Burkholderia pseudomallei and related bacteria by multiple-locus sequence typing-derived PCR and real-time PCR |journal=Journal of Clinical Microbiology |volume=45 |issue=3 |pages=1045–8 |date=March 2007 |pmid=17251403 |pmc=1829090 |doi=10.1128/JCM.02350-06 }} 27. ^{{cite journal|vauthors=Hagen RM, Frickmann H, Elschner M | title = Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples| journal = Int J Med Microbiol| year = 2011| pmid = 21658996| doi=10.1016/j.ijmm.2011.04.017| volume=301| issue=7| pages=585–90|display-authors=etal}} 28. ^{{cite journal |vauthors=Wuthiekanun V, Desakorn V, Wongsuvan G |title=Rapid immunofluorescence microscopy for diagnosis of melioidosis |journal=Clinical and Diagnostic Laboratory Immunology |volume=12 |issue=4 |pages=555–6 |date=April 2005 |pmid=15817767 |pmc=1074392 |doi=10.1128/CDLI.12.4.555-556.2005 |display-authors=etal}} 29. ^{{cite journal|title=Studies on certain biological characteristics of Malleomyces mallei and Malleomyces pseudomallei: I. Morphology, cultivation, viability, and isolation from contaminated specimens|journal=J Bacteriol|volume=55|pages=115–126|year=1948|pmid=16561426|last1=Miller|first1=WR|last2=Pannell|first2=L|last3=Cravitz|first3=L|last4=Tanner|first4=WA|last5=Ingalls|first5=MS|issue=1|pmc=518415}} 30. ^{{cite journal|title=Perasafe, Virkon and bleach are bactericidal for Burkholderia pseudomallei, a select agent and the cause of melioidosis|vauthors=Wuthiekanun V, Wongsuwan G, Pangmee S, Teerawattanasook N, Day NP, Peacock SJ |journal=J Hosp Infect|volume=77|issue=2|pages=183–4|year=2010|pmid= 20832143|pmc=3036794|doi=10.1016/j.jhin.2010.06.026}} 31. ^{{Cite journal|title = UV Light Inactivation of Bacterial Biothreat Agents|journal = Applied and Environmental Microbiology|date = 2009-05-01|issn = 0099-2240|pmc = 2681683|pmid = 19270145|pages = 2987–2990|volume = 75|issue = 9|doi = 10.1128/AEM.02180-08|first = L. J.|last = Rose|first2 = H.|last2 = O'Connell}} 32. ^{{cite journal|vauthors=Howard K, Inglis TJ |title=The effect of free chlorine on Burkholderia pseudomallei in potable water|journal=Water Res|volume=37|issue=18|pages=4425–32|year=2003|doi=10.1016/S0043-1354(03)00440-8 |pmid=14511713 |url=http://linkinghub.elsevier.com/retrieve/pii/S0043-1354(03)00440-8}} 33. ^{{cite journal|vauthors=Howard K, Inglis TJ |title=Disinfection of Burkholderia pseudomallei in potable water|journal=Water Res|volume=39|issue=6|pages=1085–92|year=2005|doi=10.1016/j.watres.2004.12.028|pmid=15766962}} 34. ^{{cite journal |vauthors=White NJ, Dance DA, Chaowagul W, Wattanagoon Y, Wuthiekanun V, Pitakwatchara N |title=Halving of mortality of severe melioidosis by ceftazidime |journal=Lancet |volume=2 |issue=8665 |pages=697–701 |date=September 1989 |pmid=2570956 |doi= 10.1016/S0140-6736(89)90768-X|url=http://linkinghub.elsevier.com/retrieve/pii/S0140-6736(89)90768-X}} 35. ^{{cite journal|vauthors=Lumbiganon P, Tattawasatra U, Chetchotisakd P |title=Comparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination|journal=J Med Assoc Thai|year=2000|volume=83|issue=8|pages=856–60|pmid=10998837|display-authors=etal}} 36. ^{{cite journal|vauthors=Wuthiekanun V, Cheng AC, Chierakul W |title=Trimethoprim/sulfamethoxazole resistance in clinical isolates of Burkholderia pseudomallei|journal=J Antimicrob Chemother|year=2005|volume=55|pages=1029–31|doi=10.1093/jac/dki151|pmid=15886263|issue=6|display-authors=etal}} 37. ^{{cite journal|vauthors=Saraya S, Soontornpas C, Chindavijak B, Mootsikapun P |title=In vitro interactions between cotrimoxazole and doxycycline in Burkholderia pseudomallei: how important is this combination in maintenance therapy of melioidosis?|journal=Indian J Med Microbiol|year=2009|volume=27|issue=1|pages=88–9|pmid=19172079}} 38. ^{{cite journal| author=Trunck LA| title=Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand| journal=PLoS Negl Trop Dis| volume=3| issue=9| pages=e519| year=2009| editor1-first=Mathieu| pmid=19771149| doi=10.1371/journal.pntd.0000519| last2=Propst| first2=KL| last3=Wuthiekanun| first3=V| editor1-last=Picardeau| last4=Tuanyok| first4=A| last5=Beckstrom-Sternberg| first5=SM| last6=Beckstrom-Sternberg| first6=JS| last7=Peacock| first7=SJ| last8=Keim| first8=P| last9=Dow| first9=SW| last10=Schweizer| first10=H. P.| pmc=2737630| display-authors=8}} 39. ^{{cite journal|title=Identification of Pseudomonas pseudomallei in the clinical laboratory|journal=J Clin Pathol|year=1979|volume=32|pages=500–4|pmid=381328|doi=10.1136/jcp.32.5.500|last1=Ashdown|first1=LR|issue=5|pmc=1145715}} 40. ^{{cite journal|vauthors=Kespichayawattana W, Intachote P, Utaisincharoen P, Stitaya Sirisinha S |title=Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells |journal=Microbial Pathogenesis|volume=36|issue=5|year=2004|pages=287–9|doi=10.1016/j.micpath.2004.01.001|pmid=15043863}} 41. ^{{cite journal|vauthors=Nandi T, Ong C, Singh AP, Boddey J, Atkins T, Sarkar-Tyson M, Essex-Lopresti AE, Chua HH, Pearson T, Kreisberg JF, Nilsson C, Ariyaratne P, Ronning C, Losada L, Ruan Y, Sung WK, Woods D, Titball RW, Beacham I, Peak I, Keim P, Nierman WC, Tan P|editor1-last=Guttman|editor1-first=David S.|title=A genomic survey of positive selection in Burkholderia pseudomallei provides insights into the evolution of accidental virulence|journal=PLoS Pathog.|year=2010|volume=6|issue=4|pages=e1000845|pmid=20368977|doi=10.1371/journal.ppat.1000845|pmc=2848565}} 42. ^1 {{cite journal |vauthors=Sim SH, Yu Y, Lin CH |editor1-last=Achtman |editor1-first=Mark |title=The core and accessory genomes of Burkholderia pseudomallei: implications for human melioidosis |journal=PLoS Pathog. |volume=4 |issue=10 |pages=e1000178 |date=October 2008 |pmid=18927621 |pmc=2564834 |doi=10.1371/journal.ppat.1000178 |display-authors=etal}} 43. ^{{cite journal|vauthors=Price EP, Hornstra HM, Limmathurotsakul D|editor1-last=Guttman|editor1-first=David S.|title=Within-host evolution of Burkholderia pseudomallei in four cases of acute melioidosis|journal=PLoS Pathog.|year=2010|volume=6|issue=1|pages=e1000725|pmid=20090837|doi=10.1371/journal.ppat.1000725|pmc=2799673|display-authors=etal}} 44. ^{{cite journal| title = Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei.|vauthors=Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ | year = 2006| volume = 4| issue = 4| pages = 272–82| pmid = 16541135| doi=10.1038/nrmicro1385| journal=Nature Reviews Microbiology}} 45. ^{{cite journal|vauthors=Kespichayawattana W, Rattanachetkul S, Wanun T |title=Burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading|journal=Infect. Immun.|year=2000|volume=68|issue=9|pages=5377–84|doi=10.1128/IAI.68.9.5377-5384.2000|pmc=101801|pmid=10948167|display-authors=etal}} 46. ^{{Cite journal|last=Toesca|first=Isabelle J.|last2=French|first2=Christopher T.|last3=Miller|first3=Jeff F.|date=2014-04-01|title=The Type VI secretion system spike protein VgrG5 mediates membrane fusion during intercellular spread by pseudomallei group Burkholderia species|journal=Infection and Immunity|volume=82|issue=4|pages=1436–1444|doi=10.1128/IAI.01367-13|issn=1098-5522|pmc=3993413|pmid=24421040}} 47. ^{{cite journal| title = A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.|vauthors=Cruz-Migoni A, Hautbergue GM, Artymiuk PJ | journal = Science| year = 2011 | volume = 334| issue = 6057| pages = 821–4| pmid = 22076380| doi =10.1126/science.1211915| pmc=3364511|display-authors=etal}} 48. ^{{cite journal |vauthors=Shalom G, Shaw JG, Thomas MS |title=In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages |journal=Microbiology |volume=153 |issue=Pt 8 |pages=2689–99 |date=August 2007 |pmid=17660433 |doi=10.1099/mic.0.2007/006585-0 |url=http://mic.sgmjournals.org/cgi/pmidlookup?view=long&pmid=17660433}} 49. ^{{cite journal|journal=Antimicrob. Agents Chemother.|volume=54|issue=8|pages=3113–20|year=2010|title=The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides, but is a broad-spectrum drug efflux system|vauthors=Mima T, Schweizer HP |pmid=20498323|pmc=2916348|doi=10.1128/AAC.01803-09}} 50. ^{{cite journal|vauthors=Norris MH, Kang Y, Lu D, Wilcox BA, Hoang TT | year = 2009| title = Glyphosate resistance as a novel select-agent-compliant, non-antibiotic-selectable marker in chromosomal mutagenesis of the essential genes asd and dapB of Burkholderia pseudomallei.| journal = Appl Environ Microbiol | volume = 75| issue = 19| pages = 6062–75| doi = 10.1128/AEM.00820-09 |pmid=19648360 |pmc=2753064 }} 51. ^{{cite journal|vauthors=Norris MH, Propst KL, Kang Y | title = The Burkholderia pseudomallei Δasd mutant exhibits attenuated intracellular infectivity and imparts protection against acute inhalation melioidosis in mice| journal = Infect Immun| year = 2011| volume = 79| issue = 10| pages = 4010–8| pmid = 21807903| doi=10.1128/IAI.05044-11| pmc=3187240|display-authors=etal}} External links
2 : Burkholderiales|Bacteria described in 1993 |
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