| 词条 | Carbol fuchsin |
| 释义 |
Carbol fuchsin, carbol-fuchsin, or carbolfuchsin, is a mixture of phenol and basic fuchsin, used in bacterial staining procedures. It is commonly used in the staining of mycobacteria as it has an affinity for the mycolic acids found in their cell membranes. It is a component of Ziehl–Neelsen stain, a differential stain.[1][2] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH).[3] Carbol-fuchsin is also used as a topical antiseptic. Its CAS number is {{CASREF|CAS=4197-24-4}}. It is also known as Castellani's paint in the US. References1. ^{{cite journal |vauthors=Angra P, Ridderhof J, Smithwick R |title=Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli |journal=J. Clin. Microbiol. |volume=41 |issue=7 |pages=3459 |date=July 2003 |pmid=12843125 |pmc=165351 |doi= 10.1128/JCM.41.7.3459.2003|url=http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=12843125}} {{Stains}}2. ^{{cite journal |vauthors=Selvakumar N, Rahman F, Rajasekaran S, Narayanan PR, Frieden TR |title=Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli |journal=J. Clin. Microbiol. |volume=40 |issue=8 |pages=3041–3 |date=August 2002 |pmid=12149374 |pmc=120628 |doi= 10.1128/JCM.40.8.3041-3043.2002|url=http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=12149374}} 3. ^{{Cite journal|last=Sokolovská|first=Ivana|last2=Rozenberg|first2=Raoul|last3=Riez|first3=Christophe|last4=Rouxhet|first4=Paul G.|last5=Agathos|first5=Spiros N.|last6=Wattiau|first6=Pierre|date=2003-12-01|title=Carbon Source-Induced Modifications in the Mycolic Acid Content and Cell Wall Permeability of Rhodococcus erythropolis E1|url=http://aem.asm.org/content/69/12/7019|journal=Applied and Environmental Microbiology|language=en|volume=69|issue=12|pages=7019–7027|doi=10.1128/AEM.69.12.7019-7027.2003|issn=0099-2240|pmid=14660344|pmc=309960}} 10 : Microscopy|Microbiology techniques|Laboratory techniques|Histopathology|Histotechnology|Staining dyes|Staining|Phenols|Antiseptics|Disinfectants |
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