“DCTN1”的意思、由来-开放百科全书

Function

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD)^[1]. Dynactin binds to cytoplasmic dynein, dynein cargo adaptors, and microtubules^[2]. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.

This subunit is commonly referred to p150-glued^[3]. It is present in two copies per dynactin complex and forms an ~75nm long flexible arm that extends from the main body of dynactin^[1]. The p150-glued arm contains binding sites for microtubules^[4], the microtubule plus tip binding protein EB1^[5], and the N-terminus of the dynein intermediate chain^[6]^[7].

Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.^[8]

Interactions

References

1. ^¹{{cite journal | vauthors = Urnavicius L, Zhang K, Diamant AG, Motz C, Schlager MA, Yu M, Patel NA, Robinson CV, Carter AP | title = The structure of the dynactin complex and its interaction with dynein | journal = Science | volume = 347 | issue = 6229 | pages = 1441–1446 | date = March 2015 | pmid = 25814576 | pmc = 4413427 | doi = 10.1126/science.aaa4080 }}
2. ^{{cite journal | vauthors = Cianfrocco MA, DeSantis ME, Leschziner AE, Reck-Peterson SL | title = Mechanism and regulation of cytoplasmic dynein | journal = Annual Review of Cell and Developmental Biology | volume = 31 | pages = 83–108 | date = 2015 | pmid = 26436706 | pmc = 4644480 | doi = 10.1146/annurev-cellbio-100814-125438 }}
3. ^¹{{cite journal | vauthors = Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK, Vallee RB | title = Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued | journal = Nature | volume = 351 | issue = 6327 | pages = 579–83 | date = June 1991 | pmid = 1828535 | pmc = | doi = 10.1038/351579a0 }}
4. ^{{cite journal | vauthors = Waterman-Storer CM, Karki S, Holzbaur EL | title = The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1) | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 92 | issue = 5 | pages = 1634–8 | date = February 1995 | pmid = 7878030 | pmc = 42574 | doi = 10.1073/pnas.92.5.1634 }}
5. ^{{cite journal | vauthors = Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE | title = The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain | journal = Current Biology | volume = 9 | issue = 8 | pages = 425–8 | date = April 1999 | pmid = 10226031 | doi = 10.1016/S0960-9822(99)80190-0 }}
6. ^{{cite journal | vauthors = Karki S, Holzbaur EL | title = Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex | journal = The Journal of Biological Chemistry | volume = 270 | issue = 48 | pages = 28806–11 | date = December 1995 | pmid = 7499404 | doi = 10.1074/jbc.270.48.28806 }}
7. ^{{cite journal | vauthors = Vaughan KT, Vallee RB | title = Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued | journal = The Journal of Cell Biology | volume = 131 | issue = 6 Pt 1 | pages = 1507–16 | date = December 1995 | pmid = 8522607 | pmc = 2120689 | doi = 10.1083/jcb.131.6.1507 }}
8. ^{{cite web | title = Entrez Gene: DCTN1 dynactin 1 (p150, glued homolog, Drosophila)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=1639| access-date = }}
9. ^{{cite journal | vauthors = Kim JC, Badano JL, Sibold S, Esmail MA, Hill J, Hoskins BE, Leitch CC, Venner K, Ansley SJ, Ross AJ, Leroux MR, Katsanis N, Beales PL | title = The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression | journal = Nature Genetics | volume = 36 | issue = 5 | pages = 462–70 | date = May 2004 | pmid = 15107855 | doi = 10.1038/ng1352 }}
10. ^{{cite journal | vauthors = Liu JJ, Ding J, Kowal AS, Nardine T, Allen E, Delcroix JD, Wu C, Mobley W, Fuchs E, Yang Y | title = BPAG1n4 is essential for retrograde axonal transport in sensory neurons | journal = The Journal of Cell Biology | volume = 163 | issue = 2 | pages = 223–9 | date = October 2003 | pmid = 14581450 | pmc = 2173519 | doi = 10.1083/jcb.200306075 }}
11. ^{{cite journal | vauthors = Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB | title = Identification of a novel 135-kDa Grb2-binding protein in osteoclasts | journal = The Journal of Biological Chemistry | volume = 271 | issue = 51 | pages = 33141–7 | date = December 1996 | pmid = 8955163 | doi = 10.1074/jbc.271.51.33141 }}
12. ^{{cite journal | vauthors = Short B, Preisinger C, Schaletzky J, Kopajtich R, Barr FA | title = The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes | journal = Current Biology | volume = 12 | issue = 20 | pages = 1792–5 | date = October 2002 | pmid = 12401177 | doi = 10.1016/s0960-9822(02)01221-6 }}

Function

Interactions

References

Further reading

External links