词条 | Dye tracing |
释义 |
Dye tracing is tracking and tracing various flows using dye added to the liquid in question. That is, it uses dye as a flow tracer. The purpose of tracking may be an analysis of the flow itself, of the transport of something by the flow of the objects that convey the flow. It is an evolution of the ages-known float tracing method, which basically consists of throwing a buoyant object into a waterflow to see where it goes or where it emerges. Dye tracking may be either qualitative, i.e., the presence of particular flow and its estimate, or quantitative, when the amount of the traced dye is measured by special instruments. Fluorescent dyesOften fluorescent dyes are used for this purpose, especially in the following circumstances
Other popular tracer dyes are rhodamine, pyranine and sulforhodamine. Quantitative tracingCarbon sampling was the first method of technology-assisted dye tracing that was based on the absorption of dye in charcoal. Charcoal packets may be placed along the expected route of the flow, later the collected dye may be chemically extracted and its amount subjectively evaluated. Filter fluorometers were the first devices that could detect dye concentrations beyond human eye sensitivity. Spectrofluorometers developed in the mid-1980s made it possible to perform advanced analysis of fluorescence. Filter fluorometers and spectrofluorometers identify the intensity of fluorescence that is present in a liquid sample. Different dyes and chemicals produce a distinctive wavelength that is determined during analysis. Tracing methodsWater flows, differentiated by the environment, possess certain factors that can affects how a dye performs. Natural fluorescence in a water flow can interfere with certain dyes. Presence of organics, other chemicals, and sunlight can influence the intensity of dyes. Each sampling area is analyzed by a quantitative instrument to test the background fluorescence. Each dye has significant performance factors that distinguish it in different settings. These factors influence how each dye interacts and is affected by each environment. Therefore, one dye can be particularly useful in one environment, whereas it could be inadequate another. These performance factors include:
ApplicationsWater tracingTypical application of water flow tracing include:[2]
Medicine and biologyDye tracing may be used for the analysis of blood circulation within various parts of the human or animal body. For example, fluorescent angiography, a technique of analysis of circulation in retina is used for diagnosing various eye diseases. With modern fluorometers, capable of tracking single fluorescent molecules, it is possible to track migrations of single cells tagged by a fluorescent molecule (see fluorescein in biological research). For example, the fluorescent-activated cell sorting in flow cytometry makes it possible to sort out the cells with attached fluorescent molecules from a flow. See also
References1. ^An educational website about karst and dye tracing, by Crawford Hydrology Laboratory / Center for Cave and Karst Study in association with Western Kentucky University {{DEFAULTSORT:Dye Tracing}}2. ^Water Tracing Dye Technical Bulletin {{webarchive|url=https://web.archive.org/web/20070203194412/http://brightdyes.com/technical/TDBGeneral.pdf |date=2007-02-03 }} 3 : Dyes|Data collection|Hydrology |
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