| 词条 | Hexadimethrine bromide |
| 释义 |
| verifiedrevid = | ImageFile = Polybrene with bromide.svg | ImageAlt = | IUPACName = 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide | PIN = | OtherNames = Polybrene |Section1={{Chembox Identifiers | CASNo_Ref = {{cascite|correct|CAS}} | CASNo = 28728-55-4 | EINECS = 684-236-5 | PubChem = | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} | ChemSpiderID = none | SMILES = }} |Section2={{Chembox Properties | Formula = (C13H30Br2N2)n, linear form | MolarMass = variable | Appearance = | Density = | MeltingPt = | BoilingPt = | Solubility = }} |Section3={{Chembox Hazards | MainHazards = | FlashPt = | AutoignitionPt = }} }}Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer used to increase the efficiency of transduction of certain cells with retrovirus in cell culture.[1] Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface.[2] Use of Polybrene can improve transduction efficiency 100-1000 fold[3] although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO.[3] It has other uses, including a role in protein sequencing.[4] Historically, hexadimethrine bromide was sometimes used to reverse heparin anticoagulation during open-heart surgery.{{Citation needed|date=September 2015}} Its use has been supplanted by protamine sulfate.{{Citation needed|date=September 2015}} Polybrene is also used in enzyme kinetic assays in order to reduce spontaneous activation of zymogens that are prone to auto activation. References1. ^{{cite journal |author1=Howard e. Davis |author2=Jeffery R. Morgan |author3=Martin L. Yarmush |title=Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes. |journal=Biophysical Chemistry |volume=97 |pages=159–172 |year=2002 |doi=10.1016/S0301-4622(02)00057-1 |issue=2}} {{genetics-stub}}{{polymer-stub}}2. ^{{cite journal |author1=Howard E. Davis |author2=Matthew Rosinski |author3=Jeffrey R. Morgan |author4=Martin L. Yarmush |title=Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation. |journal=Biophysical Journal |volume=86 |pages=1234–42 |year=2004 |pmid=14747357 |doi=10.1016/S0006-3495(04)74197-1 |issue=2 |pmc=1303915|display-authors=etal}} 3. ^1 {{Cite web|url=https://www.emdmillipore.com/US/en/product/Polybrene-Infection-/-Transfection-Reagent,MM_NF-TR-1003-G?ReferrerURL=https://www.google.com/|title=Polybrene Infection / Transfection Reagent {{!}} TR-1003-G|website=www.emdmillipore.com|access-date=2016-12-02}} 4. ^{{Cite journal|last=Hunkapiller|first=M. W.|last2=Hood|first2=L. E.|date=1978-05-30|title=Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography|journal=Biochemistry|volume=17|issue=11|pages=2124–2133|issn=0006-2960|pmid=667015}} 1 : Organic polymers |
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