| 词条 | HIS3 |
| 释义 |
| Name = Imidazoleglycerol-phosphate dehydratase | image = | width = | caption = | Organism = Saccharomyces cerevisiae | TaxID = 559292 | Symbol = HIS3 | AltSymbols = | EntrezGene = 854377 | HomoloGene = 6979 | PDB = | RefSeqmRNA = NM_001183621.1 | RefSeqProtein = NP_014845.1 | UniProt = P06633 | ECnumber = 4.2.1.19 | Chromosome = XV | EntrezChromosome = NC_001147.6 | GenLoc_start = 721846 | GenLoc_end = 722706 }} The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.[1] It is analogous to hisB in Escherichia coli. ExploitsMutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place.[2] References1. ^HIS3/YOR202W Summary {{gene-stub}}2. ^{{cite journal | vauthors = Joung JK, Ramm EI, Pabo CO | title = A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 97 | issue = 13 | pages = 7382–7 | date = June 2000 | pmid = 10852947 | pmc = 16554 | doi = 10.1073/pnas.110149297 | doi-access = free }} 1 : Saccharomyces cerevisiae genes |
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