J27 may refer to:

• County Route J27 (California), a County routes in California
• {{HMS|Blackpool|J27}}, a 1940 British Royal Navy Bangor-class minesweeper
• LNER Class J27, a 1905 British class of 0-6-0 steam locomotives
• J/27, a 1983 active sailboat racing class
• January 27, 2007 anti-war protest, an anti-war march sponsored by United for Peace and Justice in Washington, D.C

• Moody-Sankey hymnbook
• Moody's Bond Rating Service
• Moody's Mega Math Challenge
• Moodys, Oklahoma
• Moody, South Australia
• Moody, Stefan
• Moody-Stuart, Mark
• Moody Tank Conservation Park
• Moody Theater
• Moody, Thomas
• Moody Yachts
• Mooers' law
• Mooga Nomu
• Mooga, Queensland
• Moog City
• Moog Co
• Moog (company)
• Moog Company
• Moogerah, Queensland
• Moog for Love
• Moog Grandmother
• Moog guitar
• Moogmemory
• Moog, Michael
• Moog Micromoog