Major Barbara may refer to:

• Major Barbara (play), by George Bernard Shaw
• Major Barbara (film), a 1941 film based on the play
• "Major Barbara," a song by the rock band Aerosmith, on the album Pandora's Box

• Sever do Vouga Municipality
• Sever Dron
• Severe Acute Malnutrition
• Severe acute malnutrition
• Severe acute respiratory syndrome coronavirus main protease
• Severe acute respiratory syndrome (SARS)
• Severe brain injury
• Severe cold
• Severe combined immunodeficiency syndrome
• Severe Congenital Neutropenia
• Severe Cyclone Adeline-Juliet (2005)
• Severe Cyclonic Storm Helen
• Severe Cyclonic Storm Mukda (2006)
• Severe dengue
• Severed feet
• Severed foot
• Severed:Forest of the Dead (film)
• Severed Hands
• Severed Head
• Severe Disablement Allowance (UK)
• Severed Souls
• Severed: The True Story of the Black Dahlia Murder
• Severed ties
• Severed (video game)
• Severe Fever with Thrombocytopenia Syndrome