“MRNA (nucleoside-2'-O-)-methyltransferase”的意思、由来-开放百科全书

词条

MRNA (nucleoside-2'-O-)-methyltransferase

释义

Structural studies
References

{{enzyme
| Name = mRNA (nucleoside-2'-O-)-methyltransferase
| EC_number = 2.1.1.57
| CAS_number = 61970-02-3
| IUBMB_EC_number = 2/1/1/57
| GO_code = 0004483
| image =
| width =
| caption =
}}{{lowercase}}

In enzymology, a mRNA (nucleoside-2'-O-)-methyltransferase ({{EC number|2.1.1.57}}) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + m₇G(5')pppR-RNA S-adenosyl-L-homocysteine + m₇G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap)

Thus, the two substrates of this enzyme are S-adenosyl methionine and m7G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppRm-RNA (mRNA containing a 2'-O-methylpurine cap).

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (nucleoside-2'-O-)-methyltransferase. Other names in common use include messenger ribonucleate nucleoside 2'-methyltransferase, and messenger RNA (nucleoside-2'-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes {{PDB link|2GA9}} and {{PDB link|2GAF}}.

References

{{cite journal | vauthors = Barbosa E, Moss B | date = 1978 | title = mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Purification and physical properties | journal = J. Biol. Chem. | volume = 253 | pages = 7692–7 | pmid = 701281 | issue = 21 }}
{{cite journal | vauthors = Barbosa E, Moss B | date = 1978 | title = mRNA(nucleoside-2'-)-methyltransferase from vaccinia virus Characteristics and substrate specificity | journal = J. Biol. Chem. | volume = 253 | pages = 7698–702 | pmid = 701282 | issue = 21 }}
{{cite journal | vauthors = Boone RF, Ensinger MJ, Moss B | date = 1977 | title = Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus | journal = J. Virol. | volume = 21 | pages = 475–83 | pmid = 833934 | issue = 2 | pmc = 353847 }}
{{cite journal | vauthors = Ensinger MJ, Martin SA, Paoletti E, Moss B | date = 1975 | title = Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 72 | pages = 2525–9 | pmid = 1058472 | doi = 10.1073/pnas.72.7.2525 | issue = 7 | pmc = 432801 }}
{{cite journal | vauthors = Groner Y, Gilboa E, Aviv H | date = 1978 | title = Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates | journal = Biochemistry | volume = 17 | pages = 977–82 | pmid = 629955 | doi = 10.1021/bi00599a005 | issue = 6 }}

2 : EC 2.1.1|Enzymes of known structure

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