| 词条 | Naringinase |
| 释义 |
Naringinase is a debittering enzyme that is used in the commercial production of citrus juices. It breaks down the compound naringin that gives citrus juices its bitter taste. It is a multienzyme complex which possesses alpha-L-rhamnosidase and beta glucosidase active centers. The E.C. No.({{EC number|3.2.1.40}}) of the naringinase and rhamnosidase are the same. First rhamnosidase breaks naringin into prunin and rhamnose. Lastly glucosidase breaks prunin into glucose and naringenin, a flavorless flavanone also found in various citrus. Ram gene is a rare gene; it is found in very few microorganisms, like some Bacillus species. It is mainly present in the Aspergillus genus, but production of naringinase from fungus is a difficult task as the growth rate of fungi is much slower than that of bacteria.[1][2][3] References1. ^{{cite journal|last1=Puri|first1=Munish|last2=Kalra|first2=Sukirti|doi=10.1007/s11274-004-5488-7|title=Purification and characterization of naringinase from a newly isolated strain of Aspergillus niger 1344 for the transformation of flavonoids|year=2005|pages=753|volume=21|journal=World Journal of Microbiology and Biotechnology|url=https://www.scribd.com/doc/14075338/Purification-and-characterization-of-naringinase-from-a-newly-isolated-strain-of-Aspergillus-niger-1344-for-the-transformation-of-flavonoids|issue=5}} 2. ^{{cite journal|url=https://www.scribd.com/doc/14075331/Research-review-paper-Production-purification-and-characterization-of-the-debittering-enzyme-naringinase|last1=Puri|journal=Biotechnology Advances|first1=M|volume=18|pages=207–17|year=2000|title= Production, purification, and characterization of the debittering enzyme naringinase|doi=10.1016/S0734-9750(00)00034-3|issue=3|pmid=14538108|last2=Banerjee|first2=UC}} 3. ^{{cite journal|last1=Puri|first1=Munish|last2=Banerjee|first2=Anirban|last3=Banerjee|first3=U.C.|title=Optimization of process parameters for the production of naringinase by Aspergillus niger MTCC 1344|url=https://www.scribd.com/doc/14075239/Optimization-of-process-parameters-for-the-production-of-naringinase-by-Aspergillus-niger-MTCC-1344|journal=Process Biochemistry|volume=40|pages=195|year=2005|doi=10.1016/j.procbio.2003.12.009}} 3 : Enzymes|Grapefruit|Flavanones metabolism |
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