| 释义 |
- Function p47 is vital to the activation of NADPH oxidase. P47 becomes heavily phosphorylated
- Interactions
- References
- Further reading
{{Infobox_gene}}Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene.[1] Function The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.[1] Genetic variability in the NCF1 gene has been found to be related to a higher chance of getting autoimmune diseases such as Sjögren's syndrome, rheumatoid arthritis and lupus erythematosus.[2] p47 is vital to the activation of NADPH oxidase. P47 becomes heavily phosphorylated Interactions Neutrophil cytosolic factor 1 has been shown to interact with: - Moesin,[3]
- Neutrophil cytosolic factor 4,[4][5][6] and
- RELA.[7]
References 1. ^1 {{cite web | title = Entrez Gene: NCF1 neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=653361| accessdate = }}
2. ^{{Cite news|url=https://medicalxpress.com/news/2017-02-complex-genetic-region-hidden-role.html|title=Study of complex genetic region finds hidden role of NCF1 in multiple autoimmune diseases|last=|first=|date=2017-02-08|newspaper=Medical Express|access-date=2017-02-09|archive-url=|archive-date=|dead-url=|work=|via=}}
3. ^{{cite journal | vauthors = Wientjes FB, Reeves EP, Soskic V, Furthmayr H, Segal AW | title = The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain | journal = Biochem. Biophys. Res. Commun. | volume = 289 | issue = 2 | pages = 382–8 | date = Nov 2001 | pmid = 11716484 | doi = 10.1006/bbrc.2001.5982 }}
4. ^{{cite journal | vauthors = Lapouge K, Smith SJ, Groemping Y, Rittinger K | title = Architecture of the p40-p47-p67phox complex in the resting state of the NADPH oxidase. A central role for p67phox | journal = J. Biol. Chem. | volume = 277 | issue = 12 | pages = 10121–8 | date = Mar 2002 | pmid = 11796733 | doi = 10.1074/jbc.M112065200 }}
5. ^{{cite journal | vauthors = Grizot S, Grandvaux N, Fieschi F, Fauré J, Massenet C, Andrieu JP, Fuchs A, Vignais PV, Timmins PA, Dagher MC, Pebay-Peyroula E | title = Small angle neutron scattering and gel filtration analyses of neutrophil NADPH oxidase cytosolic factors highlight the role of the C-terminal end of p47phox in the association with p40phox | journal = Biochemistry | volume = 40 | issue = 10 | pages = 3127–33 | date = Mar 2001 | pmid = 11258927 | doi = 10.1021/bi0028439}}
6. ^{{cite journal | vauthors = Sathyamoorthy M, de Mendez I, Adams AG, Leto TL | title = p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain | journal = J. Biol. Chem. | volume = 272 | issue = 14 | pages = 9141–6 | date = Apr 1997 | pmid = 9083043 | doi = 10.1074/jbc.272.14.9141}}
7. ^{{cite journal | vauthors = Gu Y, Xu YC, Wu RF, Nwariaku FE, Souza RF, Flores SC, Terada LS | title = p47phox participates in activation of RelA in endothelial cells | journal = J. Biol. Chem. | volume = 278 | issue = 19 | pages = 17210–7 | date = May 2003 | pmid = 12618429 | doi = 10.1074/jbc.M210314200 }}
Further reading {{refbegin | 2}}- {{cite journal | vauthors = Dorseuil O, Gacon G | title = [Signal transduction by Rac small G proteins in phagocytes] | journal = C. R. Séances Soc. Biol. Fil. | volume = 191 | issue = 2 | pages = 237–46 | year = 1997 | pmid = 9255350 | doi = }}
- {{cite journal | vauthors = Casimir CM, Bu-Ghanim HN, Rodaway AR, Bentley DL, Rowe P, Segal AW | title = Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 88 | issue = 7 | pages = 2753–7 | year = 1991 | pmid = 2011585 | pmc = 51317 | doi = 10.1073/pnas.88.7.2753 }}
- {{cite journal | vauthors = Rodaway AR, Teahan CG, Casimir CM, Segal AW, Bentley DL | title = Characterization of the 47-kilodalton autosomal chronic granulomatous disease protein: tissue-specific expression and transcriptional control by retinoic acid | journal = Mol. Cell. Biol. | volume = 10 | issue = 10 | pages = 5388–96 | year = 1990 | pmid = 2398896 | pmc = 361238 | doi = 10.1128/mcb.10.10.5388}}
- {{cite journal | vauthors = Lomax KJ, Leto TL, Nunoi H, Gallin JI, Malech HL | title = Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease | journal = Science | volume = 245 | issue = 4916 | pages = 409–12 | year = 1989 | pmid = 2547247 | doi = 10.1126/science.2547247 }}
- {{cite journal | vauthors = Volpp BD, Nauseef WM, Donelson JE, Moser DR, Clark RA | title = Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 86 | issue = 18 | pages = 7195–9 | year = 1989 | pmid = 2550933 | pmc = 298023 | doi = 10.1073/pnas.86.18.7195 }}
- {{cite journal | vauthors = Volpp BD, Nauseef WM, Clark RA | title = Two cytosolic neutrophil oxidase components absent in autosomal chronic granulomatous disease | journal = Science | volume = 242 | issue = 4883 | pages = 1295–7 | year = 1988 | pmid = 2848318 | doi = 10.1126/science.2848318 }}
- {{cite journal | vauthors = Miki Y, Swensen J, Shattuck-Eidens D, Futreal PA, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett LM, Ding W | title = A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 | journal = Science | volume = 266 | issue = 5182 | pages = 66–71 | year = 1994 | pmid = 7545954 | doi = 10.1126/science.7545954 }}
- {{cite journal | vauthors = Leto TL, Adams AG, de Mendez I | title = Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 91 | issue = 22 | pages = 10650–4 | year = 1994 | pmid = 7938008 | pmc = 45079 | doi = 10.1073/pnas.91.22.10650 }}
- {{cite journal | vauthors = el Benna J, Faust LP, Babior BM | title = The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases | journal = J. Biol. Chem. | volume = 269 | issue = 38 | pages = 23431–6 | year = 1994 | pmid = 8089108 | doi = }}
- {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1-2 | pages = 171–4 | year = 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
- {{cite journal | vauthors = Finan PM, Hall A, Kellie S | title = Sam68 from an immortalised B-cell line associates with a subset of SH3 domains | journal = FEBS Lett. | volume = 389 | issue = 2 | pages = 141–4 | year = 1996 | pmid = 8766817 | doi = 10.1016/0014-5793(96)00552-2 }}
- {{cite journal | vauthors = Sathyamoorthy M, de Mendez I, Adams AG, Leto TL | title = p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain | journal = J. Biol. Chem. | volume = 272 | issue = 14 | pages = 9141–6 | year = 1997 | pmid = 9083043 | doi = 10.1074/jbc.272.14.9141 }}
- {{cite journal | vauthors = Görlach A, Lee PL, Roesler J, Hopkins PJ, Christensen B, Green ED, Chanock SJ, Curnutte JT | title = A p47-phox pseudogene carries the most common mutation causing p47-phox- deficient chronic granulomatous disease | journal = J. Clin. Invest. | volume = 100 | issue = 8 | pages = 1907–18 | year = 1997 | pmid = 9329953 | pmc = 508379 | doi = 10.1172/JCI119721 }}
- {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1-2 | pages = 149–56 | year = 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
- {{cite journal | vauthors = Izuhara K, Arinobu Y, Sumimoto H, Nunoi H, Takeya R, Higuchi K, Takeshige K, Hamasaki N, Harada N | title = Association of the interleukin-4 receptor alpha chain with p47phox, an activator of the phagocyte NADPH oxidase in B cells | journal = Mol. Immunol. | volume = 36 | issue = 1 | pages = 45–52 | year = 1999 | pmid = 10369419 | doi = 10.1016/S0161-5890(98)00111-4 }}
- {{cite journal | vauthors = Nakamura F, Huang L, Pestonjamasp K, Luna EJ, Furthmayr H | title = Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositides | journal = Mol. Biol. Cell | volume = 10 | issue = 8 | pages = 2669–85 | year = 1999 | pmid = 10436021 | pmc = 25498 | doi = 10.1091/mbc.10.8.2669 }}
- {{cite journal | vauthors = Rinckel LA, Faris SL, Hitt ND, Kleinberg ME | title = Rac1 disrupts p67phox/p40phox binding: a novel role for Rac in NADPH oxidase activation | journal = Biochem. Biophys. Res. Commun. | volume = 263 | issue = 1 | pages = 118–22 | year = 1999 | pmid = 10486263 | doi = 10.1006/bbrc.1999.1334 }}
- {{cite journal | vauthors = Chanock SJ, Roesler J, Zhan S, Hopkins P, Lee P, Barrett DT, Christensen BL, Curnutte JT, Görlach A | title = Genomic structure of the human p47-phox (NCF1) gene | journal = Blood Cells Mol. Dis. | volume = 26 | issue = 1 | pages = 37–46 | year = 2000 | pmid = 10772875 | doi = 10.1006/bcmd.2000.0274 }}
- {{cite journal | vauthors = Onofri F, Giovedi S, Kao HT, Valtorta F, Bongiorno Borbone L, De Camilli P, Greengard P, Benfenati F | title = Specificity of the binding of synapsin I to Src homology 3 domains | journal = J. Biol. Chem. | volume = 275 | issue = 38 | pages = 29857–67 | year = 2000 | pmid = 10899172 | doi = 10.1074/jbc.M006018200 }}
{{refend}}{{PDB Gallery|geneid=653361}}{{NADH or NADPH oxidoreductases}}{{protein-stub}} |