No Laughing Matter may refer to:

• No Laughing Matter (book), an autobiographical work by Joseph Heller about his Guillain–Barré syndrome
• "No Laughing Matter", an episode from season nine of the MASH TV series
• "No Laughing Matter" (Garfield and Friends), an episode from the second season of Garfield and Friends

• Thiopropazate
• Thioproperazine
• Thiopsyche
• Thioptera
• Thioptera nigrofimbria
• Thiopyran
• Thiopyrylium
• Thioredoxin domain
• Thioredoxin family
• Thioredoxin-Interacting Protein
• Thiorphan
• THIORPHAN
• Thiosalicylate
• Thiosalicylic acid
• Thioseconal
• Thioselenide
• Thiosemicarbazide
• Thiostrepton
• Thiosulfate-citrate-bile salts-sucrose agar
• Thiosulfate citrate bile sucrose
• Thiosulfate--dithiol sulfurtransferase
• Thiosulfate--thiol sulfurtransferase
• Thiosulfinate
• Thiosulfoxide
• Thiosulfuric acid dihydroxidooxidosulfidosulfur