| 释义 |
- Function
- Interactions
- References
- Further reading
{{Infobox_gene}}Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene.[1] Function Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is composed of approximately 30 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.[2] Interactions NUP98 has been shown to interact with: - CREB-binding protein,[3]
- KPNB1,[4][5]
- NUP88,[6]
- RAE1,[7] and
- TNPO2.[8]
References 1. ^{{cite journal | vauthors = Arai Y, Hosoda F, Kobayashi H, Arai K, Hayashi Y, Kamada N, Kaneko Y, Ohki M | title = The inv(11)(p15q22) chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene, NUP98, with the putative RNA helicase gene, DDX10 | journal = Blood | volume = 89 | issue = 11 | pages = 3936–44 | date = June 1997 | pmid = 9166830 | pmc = | doi = }}
2. ^{{cite web | title = Entrez Gene: NUP98 nucleoporin 98kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4928| accessdate = }}
3. ^{{cite journal | vauthors = Kasper LH, Brindle PK, Schnabel CA, Pritchard CE, Cleary ML, van Deursen JM | title = CREB binding protein interacts with nucleoporin-specific FG repeats that activate transcription and mediate NUP98-HOXA9 oncogenicity | journal = Mol. Cell. Biol. | volume = 19 | issue = 1 | pages = 764–76 | date = January 1999 | pmid = 9858599 | pmc = 83933 | doi = 10.1128/mcb.19.1.764}}
4. ^{{cite journal | vauthors = Moroianu J, Blobel G, Radu A | title = RanGTP-mediated nuclear export of karyopherin alpha involves its interaction with the nucleoporin Nup153 | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 94 | issue = 18 | pages = 9699–704 | date = September 1997 | pmid = 9275187 | pmc = 23253 | doi = 10.1073/pnas.94.18.9699}}
5. ^{{cite journal | vauthors = Bonifaci N, Moroianu J, Radu A, Blobel G | title = Karyopherin beta2 mediates nuclear import of a mRNA binding protein | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 94 | issue = 10 | pages = 5055–60 | date = May 1997 | pmid = 9144189 | pmc = 24630 | doi = 10.1073/pnas.94.10.5055}}
6. ^{{cite journal | vauthors = Griffis ER, Xu S, Powers MA | title = Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes | journal = Mol. Biol. Cell | volume = 14 | issue = 2 | pages = 600–10 | date = February 2003 | pmid = 12589057 | pmc = 149995 | doi = 10.1091/mbc.E02-09-0582 }}
7. ^{{cite journal | vauthors = Pritchard CE, Fornerod M, Kasper LH, van Deursen JM | title = RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains | journal = J. Cell Biol. | volume = 145 | issue = 2 | pages = 237–54 | date = April 1999 | pmid = 10209021 | pmc = 2133102 | doi = 10.1083/jcb.145.2.237}}
8. ^{{cite journal | vauthors = Fontoura BM, Blobel G, Yaseen NR | title = The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import | journal = J. Biol. Chem. | volume = 275 | issue = 40 | pages = 31289–96 | date = October 2000 | pmid = 10875935 | doi = 10.1074/jbc.M004651200 }}
{{Clear}} Further reading {{refbegin | 2}}- {{cite journal | vauthors = Slape C, Aplan PD | title = The role of NUP98 gene fusions in hematologic malignancy | journal = Leuk. Lymphoma | volume = 45 | issue = 7 | pages = 1341–50 | year = 2004 | pmid = 15359631 | doi = 10.1080/10428190310001659325 }}
- {{cite journal | vauthors = Moore MA, Chung KY, Plasilova M, Schuringa JJ, Shieh JH, Zhou P, Morrone G | title = NUP98 dysregulation in myeloid leukemogenesis | journal = Ann. N. Y. Acad. Sci. | volume = 1106 | issue = | pages = 114–42 | year = 2007 | pmid = 17442773 | doi = 10.1196/annals.1392.019 }}
- {{cite journal | vauthors = Radu A, Moore MS, Blobel G | title = The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex | journal = Cell | volume = 81 | issue = 2 | pages = 215–22 | year = 1995 | pmid = 7736573 | doi = 10.1016/0092-8674(95)90331-3 }}
- {{cite journal | vauthors = Nakamura T, Largaespada DA, Lee MP, Johnson LA, Ohyashiki K, Toyama K, Chen SJ, Willman CL, Chen IM, Feinberg AP, Jenkins NA, Copeland NG, Shaughnessy JD | title = Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia | journal = Nat. Genet. | volume = 12 | issue = 2 | pages = 154–8 | year = 1996 | pmid = 8563753 | doi = 10.1038/ng0296-154 }}
- {{cite journal | vauthors = Borrow J, Shearman AM, Stanton VP, Becher R, Collins T, Williams AJ, Dubé I, Katz F, Kwong YL, Morris C, Ohyashiki K, Toyama K, Rowley J, Housman DE | title = The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9 | journal = Nat. Genet. | volume = 12 | issue = 2 | pages = 159–67 | year = 1996 | pmid = 8563754 | doi = 10.1038/ng0296-159 }}
- {{cite journal | vauthors = Hu T, Guan T, Gerace L | title = Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins | journal = J. Cell Biol. | volume = 134 | issue = 3 | pages = 589–601 | year = 1996 | pmid = 8707840 | pmc = 2120945 | doi = 10.1083/jcb.134.3.589 }}
- {{cite journal | vauthors = Stutz F, Izaurralde E, Mattaj IW, Rosbash M | title = A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus | journal = Mol. Cell. Biol. | volume = 16 | issue = 12 | pages = 7144–50 | year = 1996 | pmid = 8943370 | pmc = 231718 | doi = 10.1128/mcb.16.12.7144}}
- {{cite journal | vauthors = Yaseen NR, Blobel G | title = Cloning and characterization of human karyopherin beta3 | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 94 | issue = 9 | pages = 4451–6 | year = 1997 | pmid = 9114010 | pmc = 20743 | doi = 10.1073/pnas.94.9.4451 }}
- {{cite journal | vauthors = Bonifaci N, Moroianu J, Radu A, Blobel G | title = Karyopherin beta2 mediates nuclear import of a mRNA binding protein | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 94 | issue = 10 | pages = 5055–60 | year = 1997 | pmid = 9144189 | pmc = 24630 | doi = 10.1073/pnas.94.10.5055 }}
- {{cite journal | vauthors = Grandi P, Dang T, Pané N, Shevchenko A, Mann M, Forbes D, Hurt E | title = Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly | journal = Mol. Biol. Cell | volume = 8 | issue = 10 | pages = 2017–38 | year = 1997 | pmid = 9348540 | pmc = 25664 | doi = 10.1091/mbc.8.10.2017 }}
- {{cite journal | vauthors = Zolotukhin AS, Felber BK | title = Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev | journal = J. Virol. | volume = 73 | issue = 1 | pages = 120–7 | year = 1999 | pmid = 9847314 | pmc = 103815 | doi = }}
- {{cite journal | vauthors = Kasper LH, Brindle PK, Schnabel CA, Pritchard CE, Cleary ML, van Deursen JM | title = CREB binding protein interacts with nucleoporin-specific FG repeats that activate transcription and mediate NUP98-HOXA9 oncogenicity | journal = Mol. Cell. Biol. | volume = 19 | issue = 1 | pages = 764–76 | year = 1999 | pmid = 9858599 | pmc = 83933 | doi = 10.1128/mcb.19.1.764}}
- {{cite journal | vauthors = Fontoura BM, Blobel G, Matunis MJ | title = A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96 | journal = J. Cell Biol. | volume = 144 | issue = 6 | pages = 1097–112 | year = 1999 | pmid = 10087256 | pmc = 2150585 | doi = 10.1083/jcb.144.6.1097 }}
- {{cite journal | vauthors = Pritchard CE, Fornerod M, Kasper LH, van Deursen JM | title = RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains | journal = J. Cell Biol. | volume = 145 | issue = 2 | pages = 237–54 | year = 1999 | pmid = 10209021 | pmc = 2133102 | doi = 10.1083/jcb.145.2.237 }}
- {{cite journal | vauthors = Ahuja HG, Felix CA, Aplan PD | title = The t(11;20)(p15;q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1 fusion | journal = Blood | volume = 94 | issue = 9 | pages = 3258–61 | year = 1999 | pmid = 10556215 | doi = }}
- {{cite journal | vauthors = Bachi A, Braun IC, Rodrigues JP, Panté N, Ribbeck K, von Kobbe C, Kutay U, Wilm M, Görlich D, Carmo-Fonseca M, Izaurralde E | title = The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates | journal = RNA | volume = 6 | issue = 1 | pages = 136–58 | year = 2000 | pmid = 10668806 | pmc = 1369901 | doi = 10.1017/S1355838200991994 }}
- {{cite journal | vauthors = Fontoura BM, Blobel G, Yaseen NR | title = The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import | journal = J. Biol. Chem. | volume = 275 | issue = 40 | pages = 31289–96 | year = 2000 | pmid = 10875935 | doi = 10.1074/jbc.M004651200 }}
- {{cite journal | vauthors = Rodrigues JP, Sitterlin D, Bachi A, Wu X, Wilm M, Carmo-Fonseca M, Izaurralde E | title = Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98 | journal = Mol. Cell | volume = 6 | issue = 5 | pages = 1243–52 | year = 2000 | pmid = 11106761 | doi = 10.1016/S1097-2765(00)00120-9 }}
- {{cite journal | vauthors = Hofmann W, Reichart B, Ewald A, Müller E, Schmitt I, Stauber RH, Lottspeich F, Jockusch BM, Scheer U, Hauber J, Dabauvalle MC | title = Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin | journal = J. Cell Biol. | volume = 152 | issue = 5 | pages = 895–910 | year = 2001 | pmid = 11238447 | pmc = 2198816 | doi = 10.1083/jcb.152.5.895 }}
{{refend}}{{PDB Gallery|geneid=4928}}{{Autoantigens}} 1 : Nuclear pore complex |