| 词条 | PMSF |
| 释义 |
| Name = Phenylmethanesulfonyl fluoride (PMSF) | verifiedrevid = 443307656 | ImageFile=PMSF.svg | ImageSize=180 | ImageFile1 = PMSF-3D-balls.png | ImageAlt1 = PMSF molecule | IUPACName=phenylmethanesulfonyl fluoride | OtherNames= |Section1={{Chembox Identifiers | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} | ChemSpiderID = 4620 | KEGG_Ref = {{keggcite|correct|kegg}} | KEGG = C06747 | InChI = 1/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2 | InChIKey = YBYRMVIVWMBXKQ-UHFFFAOYAF | ChEMBL_Ref = {{ebicite|correct|EBI}} | ChEMBL = 190503 | StdInChI_Ref = {{stdinchicite|correct|chemspider}} | StdInChI = 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2 | StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} | StdInChIKey = YBYRMVIVWMBXKQ-UHFFFAOYSA-N | CASNo_Ref = {{cascite|correct|CAS}} | CASNo=329-98-6 | PubChem=4784 | ChEBI_Ref = {{ebicite|correct|EBI}} | ChEBI = 8102 | SMILES = O=S(F)(=O)Cc1ccccc1 | MeSHName=Phenylmethylsulfonyl+fluoride |Section2={{Chembox Properties | C=7 | H=7 | F=1 | O=2 | S=1 | Appearance= | Density= | MeltingPt= | BoilingPt= | Solubility= |Section3={{Chembox Hazards | MainHazards= | FlashPt= | AutoignitionPt = }} In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases{{Citation needed|date=January 2011}}. The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C).[1] Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use. PMSF binds specifically to the active site serine residue in serine hydrolases. It does not bind to any other serine residues in the protein. This is a result of the hyperactivity of that serine residue caused by the specific environmental conditions in the enzyme's active site (catalytic triad). Because PMSF binds covalently to the enzyme, the complex can be viewed by X-ray crystallography; it can therefore be used as a chemical label to identify an essential active site serine in an enzyme. Enzyme(active)Ser-O-H + F-SO2CH2C6H5 → EnzymeSer-O-SO2CH2C6H5 + HF Serine protease + PMSF → Irreversible enzyme-PMS complex + HF The median lethal dose is less than 500 mg/kg{{Citation needed|date=September 2015}} (acetylcholine esterase inactivator!). PMSF should be handled only inside a fume hood and while wearing gloves. DMSO is sometimes recommended as solvent for stock solutions, but should not be used as it makes intact skin permeable for PMSF. References1. ^{{cite journal | author = GT James | journal = Analytical Biochemistry | volume = 86 | issue = 2 | pages = 574–9 | year = 1978 | title = Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers | pmid = 26289 | doi = 10.1016/0003-2697(78)90784-4}} External links
3 : Protease inhibitors|Serine protease inhibitors|Sulfonyl halides |
| 随便看 |
|
开放百科全书收录14589846条英语、德语、日语等多语种百科知识，基本涵盖了大多数领域的百科知识，是一部内容自由、开放的电子版国际百科全书。