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词条 POLR2A
释义

  1. Function

  2. Interactions

  3. References

  4. Further reading

{{Infobox_gene}}

DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene.

Function

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA-binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.[1]

Interactions

POLR2A has been shown to interact with:

{{div col|colwidth=20em}}
  • BRCA1,[2][3][4][5]
  • CREBBP,[6]
  • CTDP1,[3]
  • CDK8,[6]
  • GTF2B,[3]
  • GTF2F1,[3][6]
  • GTF2H4,[3]
  • MED21,[3][6][7]
  • MED26,[8]
  • PCAF,[6]
  • POLR2C,[18]
  • POLR2E,[18]
  • POLR2H,[9]
  • POLR2L,[9]
  • PQBP1,[10]
  • SMARCA2,[6][11][12]
  • SMARCA4[11][12][13]
  • SMARCB1,[6][11][12]
  • SMYD3,[14]
  • SND1,[15]
  • SUPT5H,[16][17]
  • TAF11,[3]
  • TBP,[3]
  • TCEA1,[18][19][20]
  • TCERG1,[21] and
  • ZNF74.[22]
{{Div col end}}

References

1. ^{{cite web | title = Entrez Gene: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5430| accessdate = }}
2. ^{{cite journal | vauthors = Krum SA, Miranda GA, Lin C, Lane TF | title = BRCA1 associates with processive RNA polymerase II | journal = J. Biol. Chem. | volume = 278 | issue = 52 | pages = 52012–20 | date = December 2003 | pmid = 14506230 | doi = 10.1074/jbc.M308418200 }}
3. ^1 2 3 4 5 6 7 {{cite journal | vauthors = Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD | title = BRCA1 is a component of the RNA polymerase II holoenzyme | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 94 | issue = 11 | pages = 5605–10 | date = May 1997 | pmid = 9159119 | pmc = 20825 | doi = 10.1073/pnas.94.11.5605}}
4. ^{{cite journal | vauthors = Chiba N, Parvin JD | title = Redistribution of BRCA1 among four different protein complexes following replication blockage | journal = J. Biol. Chem. | volume = 276 | issue = 42 | pages = 38549–54 | date = October 2001 | pmid = 11504724 | doi = 10.1074/jbc.M105227200 }}
5. ^{{cite journal | vauthors = Krum SA, Womack JE, Lane TF | title = Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity | journal = Oncogene | volume = 22 | issue = 38 | pages = 6032–44 | date = September 2003 | pmid = 12955082 | doi = 10.1038/sj.onc.1206515 }}
6. ^1 2 3 4 5 6 {{cite journal | vauthors = Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D | title = A human RNA polymerase II complex containing factors that modify chromatin structure | journal = Mol. Cell. Biol. | volume = 18 | issue = 9 | pages = 5355–63 | date = September 1998 | pmid = 9710619 | pmc = 109120 | doi = }}
7. ^{{cite journal | vauthors = Suñé C, Hayashi T, Liu Y, Lane WS, Young RA, Garcia-Blanco MA | title = CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription | journal = Mol. Cell. Biol. | volume = 17 | issue = 10 | pages = 6029–39 | date = October 1997 | pmid = 9315662 | pmc = 232452 | doi = }}
8. ^{{cite journal | vauthors = Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC | title = A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology | journal = Mol. Cell | volume = 14 | issue = 5 | pages = 685–91 | date = June 2004 | pmid = 15175163 | doi = 10.1016/j.molcel.2004.05.006 }}
9. ^1 2 3 {{cite journal | vauthors = Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M | title = Interactions between the human RNA polymerase II subunits | journal = J. Biol. Chem. | volume = 272 | issue = 27 | pages = 16815–21 | date = July 1997 | pmid = 9201987 | doi = 10.1074/jbc.272.27.16815}}
10. ^{{cite journal | vauthors = Humbert S, Saudou F | title = Toward cell specificity in SCA1 | journal = Neuron | volume = 34 | issue = 5 | pages = 669–70 | date = May 2002 | pmid = 12062012 | doi = 10.1016/s0896-6273(02)00715-8}}
11. ^1 2 {{cite journal | vauthors = Sif S, Saurin AJ, Imbalzano AN, Kingston RE | title = Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes | journal = Genes Dev. | volume = 15 | issue = 5 | pages = 603–18 | date = March 2001 | pmid = 11238380 | pmc = 312641 | doi = 10.1101/gad.872801 }}
12. ^1 2 {{cite journal | vauthors = Wang W, Côté J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL, Crabtree GR | title = Purification and biochemical heterogeneity of the mammalian SWI-SNF complex | journal = EMBO J. | volume = 15 | issue = 19 | pages = 5370–82 | date = October 1996 | pmid = 8895581 | pmc = 452280 | doi = }}
13. ^{{cite journal | vauthors = Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR | title = Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling | journal = Cell | volume = 95 | issue = 5 | pages = 625–36 | date = November 1998 | pmid = 9845365 | doi = 10.1016/s0092-8674(00)81633-5}}
14. ^{{cite journal | vauthors = Hamamoto R, Furukawa Y, Morita M, Iimura Y, Silva FP, Li M, Yagyu R, Nakamura Y | title = SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells | journal = Nat. Cell Biol. | volume = 6 | issue = 8 | pages = 731–40 | date = August 2004 | pmid = 15235609 | doi = 10.1038/ncb1151 }}
15. ^{{cite journal | vauthors = Yang J, Aittomäki S, Pesu M, Carter K, Saarinen J, Kalkkinen N, Kieff E, Silvennoinen O | title = Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II | journal = EMBO J. | volume = 21 | issue = 18 | pages = 4950–8 | date = September 2002 | pmid = 12234934 | pmc = 126276 | doi = 10.1093/emboj/cdf463}}
16. ^{{cite journal | vauthors = Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA | title = Tat-SF1 protein associates with RAP30 and human SPT5 proteins | journal = Mol. Cell. Biol. | volume = 19 | issue = 9 | pages = 5960–8 | date = September 1999 | pmid = 10454543 | pmc = 84462 | doi = 10.1128/mcb.19.9.5960}}
17. ^{{cite journal | vauthors = Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H | title = DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs | journal = Genes Dev. | volume = 12 | issue = 3 | pages = 343–56 | date = February 1998 | pmid = 9450929 | pmc = 316480 | doi = 10.1101/gad.12.3.343}}
18. ^{{cite journal | vauthors = Conaway RC, Kong SE, Conaway JW | title = TFIIS and GreB: two like-minded transcription elongation factors with sticky fingers | journal = Cell | volume = 114 | issue = 3 | pages = 272–4 | date = August 2003 | pmid = 12914690 | doi = 10.1016/S0092-8674(03)00607-X }}
19. ^{{cite journal | vauthors = Archambault J, Pan G, Dahmus GK, Cartier M, Marshall N, Zhang S, Dahmus ME, Greenblatt J | title = FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO | journal = J. Biol. Chem. | volume = 273 | issue = 42 | pages = 27593–601 | date = October 1998 | pmid = 9765293 | doi = 10.1074/jbc.273.42.27593}}
20. ^{{cite journal | vauthors = Pan G, Aso T, Greenblatt J | title = Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators | journal = J. Biol. Chem. | volume = 272 | issue = 39 | pages = 24563–71 | date = September 1997 | pmid = 9305922 | doi = 10.1074/jbc.272.39.24563}}
21. ^{{cite journal | vauthors = Carty SM, Goldstrohm AC, Suñé C, Garcia-Blanco MA, Greenleaf AL | title = Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase II | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 97 | issue = 16 | pages = 9015–20 | date = August 2000 | pmid = 10908677 | pmc = 16813 | doi = 10.1073/pnas.160266597 }}
22. ^{{cite journal | vauthors = Grondin B, Côté F, Bazinet M, Vincent M, Aubry M | title = Direct interaction of the KRAB/Cys2-His2 zinc finger protein ZNF74 with a hyperphosphorylated form of the RNA polymerase II largest subunit | journal = J. Biol. Chem. | volume = 272 | issue = 44 | pages = 27877–85 | date = October 1997 | pmid = 9346935 | doi = 10.1074/jbc.272.44.27877}}

Further reading

{{refbegin | 2}}
  • {{cite journal | author = Jeang KT | title = Tat, Tat-associated kinase, and transcription | journal = J. Biomed. Sci. | volume = 5 | issue = 1 | pages = 24–7 | year = 1998 | pmid = 9570510 | doi = 10.1007/BF02253352 }}
  • {{cite journal | vauthors = Yankulov K, Bentley D | title = Transcriptional control: Tat cofactors and transcriptional elongation | journal = Curr. Biol. | volume = 8 | issue = 13 | pages = R447–9 | year = 1998 | pmid = 9651670 | doi = 10.1016/S0960-9822(98)70289-1 }}
  • {{cite journal | vauthors = Romano G, Kasten M, De Falco G, Micheli P, Khalili K, Giordano A | title = Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression | journal = J. Cell. Biochem. | volume = 75 | issue = 3 | pages = 357–68 | year = 2000 | pmid = 10536359 | doi = 10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K }}
  • {{cite journal | vauthors = Szentirmay MN, Sawadogo M | title = Spatial organization of RNA polymerase II transcription in the nucleus | journal = Nucleic Acids Res. | volume = 28 | issue = 10 | pages = 2019–25 | year = 2000 | pmid = 10773068 | pmc = 105382 | doi = 10.1093/nar/28.10.2019 }}
  • {{cite journal | vauthors = Marcello A, Zoppé M, Giacca M | title = Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator | journal = IUBMB Life | volume = 51 | issue = 3 | pages = 175–81 | year = 2002 | pmid = 11547919 | doi = 10.1080/152165401753544241 }}
{{refend}}
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