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词条 Polymeric immunoglobulin receptor
释义

  1. Structure

  2. Function

      Transcytosis    Elimination of Immune Complexes  

  3. Regulation

      Cytokinetic Regulation    Hormonal Regulation    Pathogenic Stimulation  

  4. References

  5. Further reading

  6. External links

{{Infobox_gene}}Polymeric immunoglobulin receptor (pIgR) is a transmembrane protein that in humans is encoded by the PIGR gene.[1] It is an Fc receptor which facilitates the transcytosis of the soluble polymeric isoforms of immunoglobulin A , and immune complexes. pIgRs are mainly located on the epithelial lining of mucosal surfaces of the gastrointestinal tract. The composition of the receptor is complex, including 6 immunoglobulin-like domains, a transmembrane region, and an intracellular domain.[2] pIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli.[2][3]

Structure

pIgR is produced among others by intestinal epithelial cells (IECs) and bronchial epithelial cells. pIgR belongs to the family of type I transmembrane proteins. The extracellular portion of the protein contains 6 domains: 5 evolutionary conserved immunoglobulin-like domains, and 1 non-homologous domain, which is involved in proteolytic cleavage of pIg-pIgR complex from the apical side of the IECs. The quite long intracellular domain of the receptor, alongside with the transmembrane region, is responsible for the transduction of highly conserved signals.[3][4] During transcytosis, an essential part of pIgR, the secretory component, is attached to the ligand and later cleaved with the ligand to form fully functioning secreted IgA.[5]

Function

Polymeric immunoglobulin receptor is responsible for transcytosis of soluble dimeric IgAs and immune complexes from the basolateral to the apical mucosal epithelial cell surface. pIgR has a strong specificity to polymeric immunoglobulins and is not responsive to monomeric immunoglobulin.[6] The ligand’s J-chain is responsible for the binding of pIgR to its ligand.[3][5]

Transcytosis

The process of transporting polymeric immunoglobulins from the basolateral to apical side, known as transcytosis, is composed of several distinct steps. Transcytosis is initiated by either the binding of dimeric IgA to the receptor or the phosphorylation of Ser-664 residue of the receptor.[4] The internalization of both free and IgA-bound pIgR is mediated by clathrin coating. The internalized receptor is transported to basolateral early endosomes. The following step of transporting the pIgR across the cell (through tubulo-vesicular compartments to apical recycling endosome) is dependent on microtubules.[4] When pIgR reaches the apical membrane, proteolytic cleavage generates either a free secretory component of SC-IgA complex , which is released to the apical lumen.[3] Cleavage occurs at the junction of the transmembrane region of the receptor and domain 5.[2]

Elimination of Immune Complexes

pIgRs are capable of capturing IgA bound to an antigen (Immune complexes (ICs)) with identical affinity as IgA and transport them to apical side. ICs result from the capture of an antigen by an antibody. IgA ICs are formed within the mucous membranes in response to foreign invasion.[7] The accumulation of ICs on the basolateral side of mucous layers can have detrimental effects. Transcytosis of IgA ICs from the formation sites represents an important mechanism of eliminating circulating antigens and minimizing their negative effects.[7][8]

Regulation

Cytokinetic Regulation

The expression of pIgR is critically regulated by the pro-inflammatory cytokines, such as IL-1, IL-4, TNF-α, and IFN-γ. The transcriptional regulation by different cytokines proceeds though similar pathways, involving the NF-kB feedback loop. Interaction of IL-1 and TNF-α with their receptors ultimately lead to transcriptional activation of PIGR gene due to nuclear translocation of NF-kB. NF-kB interacts with intron 1 of the PIGR gene to start pIgR mRNA synthesis.[2]

Besides NF-kB pathway, the transcriptional induction also proceeds in response to IFN-γ, upregulating the expression of pIgR.[2][3]

Additionally, instead of the usual antagonistic behavior, IL-4 acts synergistically with IFN-γ to induce pIgR transcription. Their combination exhibits an upregulating effect in PIGR expression because of the presence of STAT6 enhancer, the main downstream effector of IL-4, binding site in PIGR's intron 1.[9]

Hormonal Regulation

The level of pIgRs in the mucosal reproductive tract is highly dependent on the activity of sex hormones and correlates with estrous cycle phases. The peaks of pIgR expression at proestrus and estrus phases are due to the dominant activity of estrogen, which acts as a pIgR agonist. The low levels of pIgR during the diestrus are linked to the downregulating activity of progesterone, which peaks during this phase and is able to reverse the activity of estrogen.[10] Androgens are the agonists of pIgR expression in both male and female reproductive tissues.[2]

5’-flanking region of the Pigr gene contains a response element to glucocorticoids. This class of hormones increases the steady mRNA expression levels of pIgR of intestinal cells.[9][11]

Prolactin elevates the levels of IRF-1 via Jak-STAT pathway. IRF-1 is known to be a direct agonist of pIgR expression. Considering this linkage, prolactin is believed to exhibit indirect upregulation of pIgR levels during pregnancy and lactation.[2]

Pathogenic Stimulation

IECs express a variety of Toll-like receptors (TLRs), activation of which ultimately leads to the pIgR upregulation during the infection.[2][3] The most prominent modulators of pIgR regulation consist of TLR4 and TLR3, which recognize bacterial lipopolysaccharide and viral dsRNA respectively. TLR4, like the majority of TLRs, transduce the signal though MyD88 adaptor and execute the function via NF-kB, which stimulates the expression of pIgR by binding to intron 1 of the gene. TLR3, on the other hand, involves the regulation by the means of IRF-1, which is able to promote the transcription of PIGR gene by binding to exon 1.[9][11]

References

1. ^{{cite web|url=https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5284|title=Entrez Gene: PIGR polymeric immunoglobulin receptor|access-date=}}
2. ^1 2 3 4 5 6 7 {{cite journal | vauthors = Kaetzel CS | title = The polymeric immunoglobulin receptor: bridging innate and adaptive immune responses at mucosal surfaces | journal = Immunological Reviews | volume = 206 | pages = 83–99 | date = August 2005 | pmid = 16048543 | doi = 10.1111/j.0105-2896.2005.00278.x }}
3. ^1 2 3 4 5 {{cite journal | vauthors = Asano M, Komiyama K | title = Polymeric immunoglobulin receptor | journal = Journal of Oral Science | volume = 53 | issue = 2 | pages = 147–56 | date = June 2011 | pmid = 21712618 }}
4. ^1 2 {{cite journal | vauthors = Mostov K | title = The polymeric immunoglobulin receptor | journal = Seminars in Cell Biology | volume = 2 | issue = 6 | pages = 411–8 | date = December 1991 | pmid = 1813030 }}
5. ^1 {{Cite book|url=http://www.worldcat.org/title/kuby-immunology/oclc/820117219|title=Kuby immunology|last=Owen|first=Judith A|last2=Punt|first2=Jenni|last3=Stranford|first3=Sharon A|last4=Jones|first4=Patricia P|last5=Kuby|first5=Janis|date=2013|publisher=W.H. Freeman|isbn=9781429219198|location=New York|language=English}}
6. ^{{cite journal | vauthors = Kaetzel CS, Blanch VJ, Hempen PM, Phillips KM, Piskurich JF, Youngman KR | title = The polymeric immunoglobulin receptor: structure and synthesis | journal = Biochemical Society Transactions | volume = 25 | issue = 2 | pages = 475–80 | date = May 1997 | pmid = 9191139 | doi = 10.1042/bst0250475 | url = http://www.biochemsoctrans.org/content/25/2/475 }}
7. ^1 {{cite journal | vauthors = Kaetzel CS, Robinson JK, Chintalacharuvu KR, Vaerman JP, Lamm ME | title = The polymeric immunoglobulin receptor (secretory component) mediates transport of immune complexes across epithelial cells: a local defense function for IgA | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 88 | issue = 19 | pages = 8796–800 | date = October 1991 | pmid = 1924341 }}
8. ^{{cite journal | vauthors = Phalipon A, Corthésy B | title = Novel functions of the polymeric Ig receptor: well beyond transport of immunoglobulins | journal = Trends in Immunology | volume = 24 | issue = 2 | pages = 55–8 | date = February 2003 | pmid = 12547499 }}
9. ^1 2 {{cite journal | vauthors = Johansen FE, Kaetzel CS | title = Regulation of the polymeric immunoglobulin receptor and IgA transport: new advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity | journal = Mucosal Immunology | volume = 4 | issue = 6 | pages = 598–602 | date = November 2011 | pmid = 21956244 | pmc = 3196803 | doi = 10.1038/mi.2011.37 }}
10. ^{{cite journal | vauthors = Kaushic C, Richardson JM, Wira CR | title = Regulation of polymeric immunoglobulin A receptor messenger ribonucleic acid expression in rodent uteri: effect of sex hormones | journal = Endocrinology | volume = 136 | issue = 7 | pages = 2836–44 | date = July 1995 | pmid = 7789308 | doi = 10.1210/endo.136.7.7789308 }}
11. ^1 {{cite journal | vauthors = Kaetzel CS | title = Cooperativity among secretory IgA, the polymeric immunoglobulin receptor, and the gut microbiota promotes host-microbial mutualism | journal = Immunology Letters | volume = 162 | issue = 2 Pt A | pages = 10–21 | date = December 2014 | pmid = 24877874 | pmc = 4246051 | doi = 10.1016/j.imlet.2014.05.008 }}

Further reading

{{refbegin | 2}}
  • {{cite journal | vauthors = Kaetzel CS | title = The polymeric immunoglobulin receptor: bridging innate and adaptive immune responses at mucosal surfaces | journal = Immunological Reviews | volume = 206 | issue = | pages = 83–99 | date = August 2005 | pmid = 16048543 | doi = 10.1111/j.0105-2896.2005.00278.x }}
  • {{cite journal | vauthors = Krajci P, Kvale D, Taskén K, Brandtzaeg P | title = Molecular cloning and exon-intron mapping of the gene encoding human transmembrane secretory component (the poly-Ig receptor) | journal = European Journal of Immunology | volume = 22 | issue = 9 | pages = 2309–15 | date = September 1992 | pmid = 1355431 | doi = 10.1002/eji.1830220920 }}
  • {{cite journal | vauthors = Krajci P, Grzeschik KH, Geurts van Kessel AH, Olaisen B, Brandtzaeg P | title = The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization | journal = Human Genetics | volume = 87 | issue = 6 | pages = 642–8 | date = October 1991 | pmid = 1682231 | doi = 10.1007/BF00201717 }}
  • {{cite journal | vauthors = Eiffert H, Quentin E, Wiederhold M, Hillemeir S, Decker J, Weber M, Hilschmann N | title = Determination of the molecular structure of the human free secretory component | journal = Biological Chemistry Hoppe-Seyler | volume = 372 | issue = 2 | pages = 119–28 | date = February 1991 | pmid = 1859628 | doi = 10.1515/bchm3.1991.372.1.119 }}
  • {{cite journal | vauthors = Bakos MA, Kurosky A, Goldblum RM | title = Characterization of a critical binding site for human polymeric Ig on secretory component | journal = Journal of Immunology | volume = 147 | issue = 10 | pages = 3419–26 | date = November 1991 | pmid = 1940346 | doi = }}
  • {{cite journal | vauthors = Krajci P, Solberg R, Sandberg M, Oyen O, Jahnsen T, Brandtzaeg P | title = Molecular cloning of the human transmembrane secretory component (poly-Ig receptor) and its mRNA expression in human tissues | journal = Biochemical and Biophysical Research Communications | volume = 158 | issue = 3 | pages = 783–9 | date = February 1989 | pmid = 2920039 | doi = 10.1016/0006-291X(89)92790-3 }}
  • {{cite journal | vauthors = Davidson MK, Le Beau MM, Eddy RL, Shows TB, DiPietro LA, Kingzette M, Hanly WC | title = Genetic mapping of the human polymeric immunoglobulin receptor gene to chromosome region 1q31----q41 | journal = Cytogenetics and Cell Genetics | volume = 48 | issue = 2 | pages = 107–11 | year = 1989 | pmid = 3197448 | doi = 10.1159/000132601 }}
  • {{cite journal | vauthors = Eiffert H, Quentin E, Decker J, Hillemeir S, Hufschmidt M, Klingmüller D, Weber MH, Hilschmann N | title = [The primary structure of human free secretory component and the arrangement of disulfide bonds] | journal = Hoppe-Seyler's Zeitschrift für Physiologische Chemie | volume = 365 | issue = 12 | pages = 1489–95 | date = December 1984 | pmid = 6526384 | doi = }}
  • {{cite journal | vauthors = Mizoguchi A, Mizuochi T, Kobata A | title = Structures of the carbohydrate moieties of secretory component purified from human milk | journal = The Journal of Biological Chemistry | volume = 257 | issue = 16 | pages = 9612–21 | date = August 1982 | pmid = 7107583 | doi = }}
  • {{cite journal | vauthors = Coyne RS, Siebrecht M, Peitsch MC, Casanova JE | title = Mutational analysis of polymeric immunoglobulin receptor/ligand interactions. Evidence for the involvement of multiple complementarity determining region (CDR)-like loops in receptor domain I | journal = The Journal of Biological Chemistry | volume = 269 | issue = 50 | pages = 31620–5 | date = December 1994 | pmid = 7989333 | doi = }}
  • {{cite journal | vauthors = Maruyama K, Sugano S | title = Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides | journal = Gene | volume = 138 | issue = 1–2 | pages = 171–4 | date = January 1994 | pmid = 8125298 | doi = 10.1016/0378-1119(94)90802-8 }}
  • {{cite journal | vauthors = Piskurich JF, France JA, Tamer CM, Willmer CA, Kaetzel CS, Kaetzel DM | title = Interferon-gamma induces polymeric immunoglobulin receptor mRNA in human intestinal epithelial cells by a protein synthesis dependent mechanism | journal = Molecular Immunology | volume = 30 | issue = 4 | pages = 413–21 | date = March 1993 | pmid = 8455639 | doi = 10.1016/0161-5890(93)90071-I }}
  • {{cite journal | vauthors = Piskurich JF, Youngman KR, Phillips KM, Hempen PM, Blanchard MH, France JA, Kaetzel CS | title = Transcriptional regulation of the human polymeric immunoglobulin receptor gene by interferon-gamma | journal = Molecular Immunology | volume = 34 | issue = 1 | pages = 75–91 | date = January 1997 | pmid = 9182878 | doi = 10.1016/S0161-5890(96)00079-X }}
  • {{cite journal | vauthors = Hughes GJ, Frutiger S, Savoy LA, Reason AJ, Morris HR, Jaton JC | title = Human free secretory component is composed of the first 585 amino acid residues of the polymeric immunoglobulin receptor | journal = FEBS Letters | volume = 410 | issue = 2–3 | pages = 443–6 | date = June 1997 | pmid = 9237679 | doi = 10.1016/S0014-5793(97)00629-7 }}
  • {{cite journal | vauthors = Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S | title = Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library | journal = Gene | volume = 200 | issue = 1–2 | pages = 149–56 | date = October 1997 | pmid = 9373149 | doi = 10.1016/S0378-1119(97)00411-3 }}
  • {{cite journal | vauthors = Ackermann LW, Wollenweber LA, Denning GM | title = IL-4 and IFN-gamma increase steady state levels of polymeric Ig receptor mRNA in human airway and intestinal epithelial cells | journal = Journal of Immunology | volume = 162 | issue = 9 | pages = 5112–8 | date = May 1999 | pmid = 10227981 | doi = }}
  • {{cite journal | vauthors = Røe M, Norderhaug IN, Brandtzaeg P, Johansen FE | title = Fine specificity of ligand-binding domain 1 in the polymeric Ig receptor: importance of the CDR2-containing region for IgM interaction | journal = Journal of Immunology | volume = 162 | issue = 10 | pages = 6046–52 | date = May 1999 | pmid = 10229845 | doi = }}
  • {{cite journal | vauthors = Norderhaug IN, Johansen FE, Krajci P, Brandtzaeg P | title = Domain deletions in the human polymeric Ig receptor disclose differences between its dimeric IgA and pentameric IgM interaction | journal = European Journal of Immunology | volume = 29 | issue = 10 | pages = 3401–9 | date = October 1999 | pmid = 10540352 | doi = 10.1002/(SICI)1521-4141(199910)29:10<3401::AID-IMMU3401>3.0.CO;2-G }}
  • {{cite journal | vauthors = Mostov KE, Friedlander M, Blobel G | title = The receptor for transepithelial transport of IgA and IgM contains multiple immunoglobulin-like domains | journal = Nature | volume = 308 | issue = 5954 | pages = 37–43 | year = 1984 | pmid = 6322002 | doi = 10.1038/308037a0 }}
  • {{cite journal | vauthors = Hughes GJ, Reason AJ, Savoy L, Jaton J, Frutiger-Hughes S | title = Carbohydrate moieties in human secretory component | journal = Biochimica et Biophysica Acta | volume = 1434 | issue = 1 | pages = 86–93 | date = September 1999 | pmid = 10556562 | doi = 10.1016/S0167-4838(99)00168-5 }}
{{refend}}

External links

  • {{MeshName|Polymeric+Immunoglobulin+Receptor}}
{{PDB Gallery|geneid=5284}}{{Immunoglobulin superfamily immune receptors}}{{Portal bar|Molecular and cellular biology}}{{NLM content}}

1 : Fc receptors
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