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词条 Roger Y. Tsien
释义

  1. Early life

  2. Education

  3. Research and career

      Fluorescent proteins    Calcium imaging    FlAsH-EDT2    Fluorescence-assisted cancer surgery    Industrial activities  

  4. Personal life

     Death 

  5. Awards and honors

      Named lectures and lectureships  

  6. References

  7. External links

{{Use mdy dates |date = November 2011}}{{Infobox scientist
|name = Roger Tsien
|birth_name = Tsien Yonchien (錢永健)
|image = Roger Tsien-press conference Dec 07th, 2008-2.jpg
|image_size =
|caption = Tsien in 2008
|birth_date = {{Birth date|1952|2|1}}
|birth_place = New York, New York, U.S.
|death_date = {{Death date and age|mf=yes|2016|8|24|1952|2|1}}
|death_place = Eugene, Oregon, U.S.[1]
|residence = San Diego, California
|citizenship = United States
|nationality = American
|ethnicity = Han Chinese/Taiwanese
|spouse = Wendy Globe[1]
|fields = Biochemistry
|workplaces = {{Plainlist|
  • {{nowrap|University of California, San Diego}}
  • University of California, Berkeley}}

|alma_mater = {{Plainlist|
  • Harvard University
  • University of Cambridge}}

|doctoral_advisor = Richard Adrian[3]
|academic_advisors =
|thesis_title = The design and use of organic chemical tools in cellular physiology
|thesis_year = 1976
|thesis_url = http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.475593
|doctoral_students =
|notable_students =
|known_for = {{Plainlist|
  • Green fluorescent protein[2]
  • Calcium imaging}}

|awards = {{Plainlist|
  • Nobel Prize in Chemistry (2008)
  • Golden Goose Award (2012)
  • E. B. Wilson Medal (2008)
  • Rosenstiel Award (2006)
  • ForMemRS (2006)[3]
  • Wolf Prize in Medicine (2004)[4]
  • Keio Medical Science Prize (2004)
  • EMBO Membership (2005)[5]
  • Dr A.H. Heineken Prize (2002)
  • Artois-Baillet Latour Health Prize (1995)
  • Gairdner Foundation International Award (1995)}}

|religion =
|signature =
|footnotes =
|website = {{URL|http://www.tsienlab.ucsd.edu}}
|module = {{Chinese |child=yes |t = 錢永健 |s = 钱永健 |p = Qián Yǒngjiàn |w = Ch'ien Yung-chien}}
}}{{Chinese name |Tsien}}Roger Yonchien Tsien ({{zh |t = 錢永健}}; February 1, 1952 – August 24, 2016) was a Chinese-American biochemist. He was a professor of chemistry and biochemistry at the University of California, San Diego[6] and was awarded the 2008 Nobel Prize in Chemistry for his discovery and development of the green fluorescent protein, in collaboration with organic chemist Osamu Shimomura and neurobiologist Martin Chalfie.[7][8][9] Tsien was also a pioneer of calcium imaging.[10][11]

Early life

Tsien was born in New York, in 1952.[14] He grew up in Livingston, New Jersey[14] and attended Livingston High School there.[12] His Han Chinese family was originally from Hangzhou, China. Hsue-Chu Tsien, his father, was a mechanical engineer had excelled academically and graduated at the top of his university class.

Tsien suffered from asthma as a child, and as a result, he was often indoors. He spent hours conducting chemistry experiments in his basement laboratory. When he was 16, he won first prize in the nationwide Westinghouse Talent Search with a project investigating how metals bind to thiocyanate.[13]

Education

Tsien attended Harvard College on a National Merit Scholarship, where he was elected to Phi Beta Kappa as a junior.[14] He graduated summa cum laude with a Bachelor of Science in chemistry and physics in 1972.[19] According to his freshman-year roommate, economist and Iowa politician Herman Quirmbach, "It's probably not an exaggeration to say he's the smartest person I ever met ... [a]nd I have met a lot of brilliant people."[15]

After completing his bachelor's degree, Tsien joined the Physiological Laboratory at the University of Cambridge in Cambridge, England with the aid of a Marshall Scholarship, and resided at Churchill College, Cambridge.[16] He received his Ph.D. in physiology in 1977 for research on The Design and Use of Organic Chemical Tools in Cellular Physiology [17] formally supervised by Richard Adrian in the Department of Physiology and assisted by Andy Holmes, Gerry Smith and Jeremy Sanders in the Department of Chemistry.[16]

Research and career

Following his Ph.D., Tsien was a Research Fellow at Gonville and Caius College, Cambridge from 1977 to 1981.[16] He was appointed to the faculty at the University of California, Berkeley, from 1982 to 1989.[18] Beginning in 1989, he worked at the University of California, San Diego, as Professor of Pharmacology and Professor of Chemistry and Biochemistry,[6] and as an investigator of the Howard Hughes Medical Institute.[19][20]

Tsien contributed to the fields of cell biology and neurobiology by discovering genetically programmable fluorescent tags, thereby allowing scientists to watch the behavior of molecules in living cells in real time. He also developed fluorescent indicators of calcium ions and other ions important in biological processes.

In 2004, Tsien was awarded the Wolf Prize in Medicine "for his seminal contribution to the design and biological application of novel fluorescent and photolabile molecules to analyze and perturb cell signal transduction."[21]

In 2008, Tsien shared the Nobel Prize in Chemistry with Osamu Shimomura and Martin Chalfie for "the green fluorescent protein: discovery, expression and development."[7][22]

Fluorescent proteins

The multicolored fluorescent proteins developed in Tsien's lab are used by scientists to track where and when certain genes are expressed in cells or in whole organisms. Typically, the gene coding for a protein of interest is fused with the gene for a fluorescent protein, which causes the protein of interest to glow inside the cell when the cell is irradiated with ultraviolet light and allows microscopists to track its location in real time. This is such a popular technique that it has added a new dimension to the fields of molecular biology, cell biology, and biochemistry.[23]

Since the discovery of the wild type GFP, numerous different mutants of GFP have been engineered and tested.[24] The first significant leap forward was a single point mutation (S65T) reported by Tsien in 1995 in Nature.[25] This mutation dramatically improved the fluorescent (both intensity and photostability) and spectral characteristics of GFP. A shift of the major excitation peak to 488 nm with the emission peak staying at 509 nm thus can be clearly observed, which matched very well the spectral characteristics of commonly available FITC facilities. All these then largely amplified the practicality of using GFP by scientists in their research. Tsien mainly contributed to much of our understanding of how GFP works and for developing new techniques and mutants of GFP.

Former trainees of Roger Y. Tsien include Atsushi Miyawaki and Alice Y. Ting.

Timelines of GFP-development involved by Tsien:[22]

  • 1994: Tsien showed the mechanism that GFP chromophore is formed in a chemical reaction which requires oxygen but without help from the other proteins.
  • 1994–1998: Tsien and collaborators made various GFP mutants by genetic modification and structural tweaking. Newly created variants of GFP can shine more brightly and show different colours, such as yellow, cyan, and blue.
  • 2000–2002: Tsien produced monomeric variants of DsRED, which can glow in shades of red, pink, and orange. Remarkably, since then complicated marcromolecular networks of living organisms can be labelled or marked by using "all the colours of the rainbow".

Other detailed highlights involved by Tsien:[26]

  • 2002: The critical structural difference between GFP and DsRed was revealed. One extra double-bond in the chromophore of DsRed extends its conjugation thus causes the red-shift.
  • 2002: Monomeric DsRed (mRFP) was first developed.
  • 2004: New "fruit" FPs were generated (by in vitro and in vivo directed evolutions).

In 2009, a new kind of Infrared Fluorescent Protein (IFP) was developed by Tsien's group, and further reported and described by Science. The new IFPs are developed from bacterial phytochromes instead of from multicellular organism like jellyfish. Under normal conditions, bacterial phytochromes absorb light for signaling instead of fluorescence, but they can be turned fluorescent after deleting some of the signaling parts by genetic means such as site-directed mutagenesis. In order to fluoresce, IFPs require an exogenous chromophore, biliverdin.[27]

In 2016, a new class of fluorescent protein was evolved from a cyanobacterial (Trichodesmium erythraeum) phycobiliprotein, α-allophycocyanin, and named small ultra red fluorescent protein (smURFP). smURFP autocatalytically self-incorporates the chromophore biliverdin without the need of an external protein, known as a lyase.[28] Jellyfish- and coral-derived fluorescent proteins require oxygen and produce a stoichiometric amount of hydrogen peroxide upon chromophore formation.[29] smURFP does not require oxygen or produce hydrogen peroxide and uses the chromophore, biliverdin. smURFP has a large extinction coefficient (180,000 M−1 cm−1) and has a modest quantum yield (0.20), which makes it comparable biophysical brightness to eGFP and ~2-fold brighter than most red or far-red fluorescent proteins derived from coral. smURFP spectral properties are similar to the organic dye Cy5.[28]

Calcium imaging

Tsien was a pioneer of calcium imaging and known for developing various dyes which become fluorescent in the presence of particular ions such as calcium.[10][11] One such dye, Fura-2, is widely used to track the movement of calcium within cells. Indo-1, another popular calcium indicator, was also developed by Tsien's group in 1985. He has also developed fluorescent indicators for other ions such as magnesium, zinc, copper, iron, lead, cadmium, aluminum, nickel, cobalt, and mercury.[30]

Aequorin is also a useful tool to indicate calcium level inside cells; however, it has some limitations, primarily is that its prosthetic group coelenterazine is consumed irreversibly when emits light, thus requires continuous addition of coelenterazine into the media. To overcome such issues, Tsien's group also developed the calmodulin-based sensor, named Cameleon.[31]

FlAsH-EDT2

FlAsH-EDT2 is a biochemical method for specific covalent labeling inside live cells. It's a method based on recombinant protein molecules, and was developed by Tsien and his colleagues in 1998.[32]
  • "FLASH-EDT2": Fluorescein arsenical helix binder, bis-EDT adduct,
  • "EDT": 1,2-ethanedithiol.

Fluorescence-assisted cancer surgery

Mouse experiments by Tsien's group suggest that cancer surgery can be guided and assisted by fluorescent peptides. The peptides are used as probes, and are harmless to living tissues and organs. Their lifetime in the body is only 4 or 5 days. Clinical trials are awaited.[33]

Industrial activities

Tsien was also a notable biochemical inventor and holds or co-holds about 100 patents till 2010. In 1996, Tsien co-founded the Aurora Biosciences Corporation, which went public in 1997. In 2001, Aurora was acquired by the Vertex Pharmaceuticals. Similarly, Tsien was also a scientific co-founder of Senomyx in 1999.[13][34]

Dr. Tsien also promoted science education to promising young scientists through the first-ever San Diego Science Festival Lunch with a Laureate Program.[35]

Personal life

According to the Qian (Tsien) clan genealogy book, Tsien is a 34th-generational descendant of King Qian Liu of the Wuyue Kingdom of ancient China. His family belongs to the line of King Qian Hongzong.[36] Tsien's parents Hsue-Chu Tsien and Yi-Ying Li (李懿颖) came from Hangzhou and Beijing, respectively.

Tsien had a number of engineers in his extended family, including his father Hsue-Chu Tsien who was an MIT-educated mechanical engineer and his mother's brothers Y. T. Li (李耀滋) and Shihying Lee (李诗颖), who were engineering professors at MIT. Tsien's mother Yi-Ying Li was a nurse.[19] The famous rocket scientist Tsien Hsue-shen, regarded as the co-founding father of the Jet Propulsion Laboratory of the California Institute of Technology and, later, the director of the Chinese ballistic-missile and space programs, is a cousin of Tsien's father.[37]

Tsien was the younger brother of Richard Tsien, a renowned scientist currently at New York University,[38] and Louis Tsien, a software engineer. Tsien, who called his own work molecular engineering, once said, "I'm doomed by heredity to do this kind of work."[39]

He was married to Wendy Globe.[1]

Death

On August 31, 2016, a press release by UC San Diego announced the death of Prof. Tsien. He died on August 24, 2016.[40] Although the actual cause of death is yet to be determined, he seemed to have died while on a bike trail in Eugene, Oregon.[41][42][43] Prior to his death, Tsien had survived cancer and suffered a stroke in 2013.[44][45]

"He was ahead of us all," said Tsien's wife, Wendy. "He was ever the adventurer, the pathfinder, the free and soaring spirit. Courage, determination, creativity and resourcefulness were hallmarks of his character. He accomplished much. He will not be forgotten."[41]

Awards and honors

Roger Y. Tsien has received numerous honors and awards in his life, including:

{{Div col|colwidth=35em}}
  • National 1st Prize, Westinghouse Science Talent Search (1968)
  • National Merit Scholarship, USA (1968)
  • Detur Prize, Harvard College (1969)
  • Marshall Scholarship, British government (1972)
  • Comyns Berkeley Research Fellowship, Gonville and Caius College, Cambridge (1977)
  • Gedge Prize, University of Cambridge (1978)
  • Searle Scholar, Searle Scholar program (1983)
  • Lamport Prize, New York Academy of Sciences (1986)
  • Javits Neuroscience Investigator Award, National Institute of Neurological Disorders and Stroke (1989)
  • Young Scientist Award, Passano Foundation (1991)
  • W. Alden Spencer Award in Neurobiology, Columbia University (1991)
  • Artois-Baillet-Latour Health Prize, Belgium (1995)
  • Gairdner Foundation International Award, Canada (1995)
  • Basic Research Prize, American Heart Association (1995)
  • Elected to the United States Institute of Medicine (1995)
  • Doctorate honoris causa, Katholieke Universiteit Leuven, Belgium (1995)
  • Faculty Research Lecturer, UC San Diego (1997)
  • Elected to the American Academy of Arts and Sciences (1998)
  • Elected to the United States National Academy of Sciences (1998)
  • Award for Innovation in High Throughput Screening, Society for Biomolecular Screening (1998)
  • Pearse Prize, Royal Microscopical Society (2000)
  • ACS Award for Creative Invention, American Chemical Society (2002)
  • Christian B. Anfinsen Award, Protein Society (2002)
  • Heineken Prize for Biochemistry and Biophysics, Royal Netherlands Academy of Arts and Sciences (2002)
  • Max Delbrück Medal, Max Delbrück Centrum für Molekulare Medizin, Berlin (2002)[46]
  • Wolf Prize in Medicine, Israel (2004)
  • Keio Medical Science Prize, Japan (2004)
  • UCSD Chancellor's Associates Award for Excellence in Science & Engineering Research, UC San Diego (2004)
  • Perl-UNC Neuroscience Prize, University of North Carolina (2004)
  • Associate EMBO Member of the European Molecular Biology Organization 2005.[5]
  • J.Allyn Taylor International Prize in Medicine, Robarts Research Institute, Canada (2005)
  • ABRF Award, Association of Biomolecular Resource Facilities (2006)
  • Lewis S. Rosenstiel Award for Distinguished Work in the Basic Medical Sciences, Brandeis University (2006)[47]
  • Elected a Foreign Member of the Royal Society (ForMemRS) in 2006[3][48]
  • BioPharma Leadership Award, the 6th Annual San Diego BioPharma Conference, San Diego (2007)
  • US Department of Defense (DoD) Breast Cancer Innovator Award
  • Nobel Prize in Chemistry, Sweden (2008)[7]
  • E.B. Wilson Medal, American Society for Cell Biology (2008)
  • Honorary Fellow of the Royal Society of Chemistry (HonFRSC), UK (2008)[49]
  • Honorary Academician, Academia Sinica (2008)[50]
  • February 18, 2009, Roger Tsien Day, in the City of San Diego, California, USA
  • Distinguished Science and Technology Award, The 2009 Asian American Engineers of the Year (AAEoY) Award (April 2009)[51]
  • Lifetime Innovation Award, UC San Diego (May 20, 2009)[52]
  • AHA Distinguished Scientists, American Heart Association (2009)
  • Molecular Imaging Achievement Award, Society of Molecular Imaging (2009)
  • Doctor of Science honoris causa, The University of Hong Kong (2009)[53]
  • Doctor of Science honoris causa, Chinese University of Hong Kong (2009)[54]
  • General President Gold Medal, the 97th Indian Science Congress, India (January 3, 2010)[55]
  • Spiers Memorial Award, Royal Society of Chemistry, UK (2010)[56]
  • Golden Goose Award (2012)[57]
{{Div col end}}

Named lectures and lectureships

{{Div col|colwidth=35em}}
  • Bowditch Lectureship, American Physiological Society (1992)[58]
  • Hans L. Falk Memorial Lectureship, National Institute of Environmental Health Sciences (1993)
  • Quastel Lectureship, Hebrew University of Jerusalem (1994)
  • President's Lectureship, American Thoracic Society (1994)
  • Roger Eckert Memorial Lecture, Göttingen Neurobiology Conference of the German Neuroscience Society (1995)
  • Melvin Calvin Lectureship, UC Berkeley (1999)
  • Herbert Sober Lectureship, American Society for Biochemistry and Molecular Biology (2000)
  • Keith Porter Lecture, American Society for Cell Biology (2003)
  • Konrad Bloch Lectureship, Harvard University (2003)
  • Grass Foundation Lectureship, Society for Neuroscience (2004)
  • The 1st Academia Sinica Lecturer (the highest honor of the academy), Dec 2009[59][60]
  • The 2010 National Lecturer of the Biophysical Society (the highest honor of the society)[61]
  • The 2011 UCL Prize Lecture in Clinical Science (University College, London)[62]
{{Div col end}}

References

1. ^1 2 {{cite web |url = https://www.washingtonpost.com/national/health-science/roger-y-tsien-chemist-shared-nobel-for-tool-to-research-alzheimers-dies-at-64/2016/08/31/90ca6de8-6fc9-11e6-8533-6b0b0ded0253_story.html |title = Roger Y. Tsien, chemist shared Nobel for tool to research Alzheimer's, dies at 64 |publisher = The Washington Post |date = 31 August 2016 |accessdate = 1 September 2016}}
2. ^{{cite journal |last=Tsien |first=Roger Y. |year=1998 |title = The Green Fluorescent Protein |journal = Annual Review of Biochemistry |volume=67 |issue=1 |pages = 509–544 |doi = 10.1146/annurev.biochem.67.1.509 |pmid = 9759496}}
3. ^1 {{cite web |author=Anon |year=2006 |publisher=Royal Society |location=London |title=Professor Roger Tsien ForMemRS |archiveurl=https://web.archive.org/web/20151117111539/https://royalsociety.org/people/roger-tsien-12438/ |archivedate=2015-11-17 |url=https://royalsociety.org/people/roger-tsien-12438/ }} One or more of the preceding sentences incorporates text from the royalsociety.org website where: {{quote|All text published under the heading 'Biography' on Fellow profile pages is available under Creative Commons Attribution 4.0 International License." --{{cite web |url=https://royalsociety.org/about-us/terms-conditions-policies/ |title=Royal Society Terms, conditions and policies |accessdate=2016-03-09 |deadurl=bot: unknown |archiveurl=https://web.archive.org/web/20150925220834/https://royalsociety.org/about-us/terms-conditions-policies/ |archivedate=September 25, 2015 |df=mdy-all }}}}
4. ^The Wolf Prize in Medicine in 2004 (detail)
5. ^1 {{cite web |url = http://people.embo.org/profile/roger-y-tsien |website = people.embo.org |title = Roger Tsien EMBO Profile |publisher = European Molecular Biology Organization |location = Heidelberg |author = Anon |year=2016}}
6. ^1 {{cite web |url = http://www-chem.ucsd.edu/research/profile.cfm?cid=C01821 |title = Roger Tsien at UCSD Department of Chemistry & Biochemistry |format = Official web page |publisher = UCSD |year = 2008 |accessdate = November 4, 2008 |deadurl = yes |archiveurl = https://web.archive.org/web/20081015182639/http://www-chem.ucsd.edu/research/profile.cfm?cid=C01821 |archivedate = October 15, 2008 |df = mdy-all }}
7. ^1 2 {{cite web |url = http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/index.html |title = 2008 Nobel Prize in Chemistry Laureates |format = Official web page |publisher = The Nobel Foundation |date = October 8, 2008 |accessdate = October 8, 2008}}
8. ^Tsien Nobel Prize lecture
9. ^{{cite journal |last1=Tsien |first1=Roger Y. |title=The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008 |journal=Journal of Visualized Experiments |issue=35 |year=2010 |issn = 1940-087X |doi = 10.3791/1575 |pmid=20072108 |pmc=3152217 }} {{open access}}
10. ^1 [https://www.nobelprize.org/nobel_prizes/chemistry/laureates/2008/tsien-bio.html Roger Y. Tsien - Biographical]
11. ^1 {{cite journal |last= Rudolf |first=Rüdiger |last2=Mongillo |first2=Marco |last3=Rizzuto |first3=Rosario |last4=Pozzan |first4=Tullio |authorlink4=Tullio Pozzan|year = 2003 |title= Looking forward to seeing calcium |url = http://csl.johnshopkins.edu/academics/580427/rsc/580427_5_measuringcellcareview_032106.pdf |journal= Nature Reviews Molecular Cell Biology |volume=4 |issue=7 |pages= 579–586 |doi= 10.1038/nrm1153 |access-date= 1 September 2016 |pmid= 12838340 }}
12. ^Swayze, Bill. "Jersey teens call science a winner: Two finalists say just being in Westinghouse talent competition is prize enough", The Star-Ledger, March 11, 1997. Accessed September 18, 2007. "Only one New Jersey teenager has ever captured top honors in the history of the competition. That was Roger Tsien in 1968. The then-16-year-old Livingston High School math-science whiz explored the way subatomic particles act as bridges between two dissimilar metal atoms in various complex molecules."
13. ^1 2 3 Nicole Kresge, Robert D. Simoni, and Robert L. Hill. "The Chemistry of Fluorescent Indicators: the Work of Roger Y. Tsien", Journal of Biological Chemistry, September 15, 2006. Accessed September 18, 2007. "At age 16, Tsien won first prize in the nationwide Westinghouse talent search with a project investigating how metals bind to thiocyanate."
14. ^{{cite web |url = http://www.thecrimson.com/article/1971/4/24/phi-beta-kappa-pthe-alpha-chapter/ |title = Phi Beta Kappa |format = Web page |publisher=The Harvard Crimson |date=April 24, 1971 |accessdate=October 9, 2008}}
15. ^{{cite web |url = http://www.thecrimson.com/article/2008/10/9/harvard-alumni-win-nobel-prize-in/ |title = Harvard Alumni Win Nobel Prize – Three chemists share award for green fluorescent jellyfish protein |format= Web page |publisher=The Harvard Crimson |author1=June Q. Wu |author2=CRIMSON STAFF WRITER |date=October 9, 2008 |accessdate=October 9, 2008}}
16. ^1 2 {{cite web |url = http://www.admin.cam.ac.uk/news/dp/2008100801 |title=Cambridge graduate wins Nobel Prize for Chemistry |format= Web page |publisher=The University of Cambridge |date=October 8, 2008 |accessdate=October 8, 2008}}
17. ^1 {{cite thesis |degree=PhD |publisher=University of Cambridge |title=The design and use of organic chemical tools in cellular physiology.|first= Roger Yongchien|last=Tsien|date=1976|url=http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.475593|oclc=500581238}}
18. ^{{cite journal |last=Rink |first= Timothy J. |last2=Tsien |first2=Louis Y.|last3=Tsien |first3=Richard W. |date=13 October 2016 |title=Roger Yonchien Tsien (1952–2016) |journal=Nature |volume=538 |issue=172 |pages= 172|doi = 10.1038/538172a |issn = 0028-0836 |pmid=27734865|pmc= 5960232 }}
19. ^{{cite web|url=http://www.hhmi.org/research/investigators/tsien.html |title=HHMI Scientist Abstract: Roger Y. Tsien, PhD |format= Web page |publisher=HHMI |date=August 20, 2007 |accessdate=October 8, 2008}}
20. ^{{cite web|url=http://www.hhmi.org/research/investigators/tsien_bio.html |title=HHMI Scientist Bio: Roger Y. Tsien, PhD |format= Web page |publisher=HHMI |accessdate=October 8, 2008}}
21. ^{{cite web|url=http://www.wolffund.org.il/cat.asp?id=24&cat_title=MEDICINE |title=The Wolf Foundation Prize in Medicine |format=Web page |publisher=The Wolf Foundation |year=2004 |accessdate=October 8, 2008}}
22. ^1 {{cite web |url=http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/chemadv08.pdf |title=The green fluorescent protein: discovery, expression and development |publisher=The Royal Swedish Academy of Sciences, Information Department |date=October 8, 2008 |accessdate=October 8, 2008 |deadurl=yes |archiveurl=https://web.archive.org/web/20081028213021/http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/chemadv08.pdf |archivedate=October 28, 2008 |df=mdy-all }}
23. ^Nobel lecture
24. ^{{cite journal |vauthors = Shaner N, Steinbach P, Tsien R |title = A guide to choosing fluorescent proteins |journal = Nat Methods |volume=2 |issue=12 |pages = 905–9 |year=2005 |url = http://tsienlab.ucsd.edu/Publications/Shaner%202005%20Nature%20Methods%20-%20Choosing%20fluorescent%20proteins.pdf |pmid=16299475 |doi = 10.1038/nmeth819}}
25. ^{{cite journal |vauthors = Heim R, Cubitt A, Tsien R |title=Improved green fluorescence |journal=Nature |volume=373 |issue=6516 |pages = 663–4 |year=1995 |url= http://tsienlab.ucsd.edu/Publications/Heim%201995%20Nature%20-%20Improved%20GFP.PDF |pmid=7854443 |doi = 10.1038/373663b0}}
26. ^{{cite web|url=http://www.conncoll.edu/ccacad/zimmer/GFP-ww/timeline.html |title=Green Fluorescent Protein: Timeline |format=Web page |publisher=Conncoll.edu |date=18 Nov 2009 |accessdate=December 22, 2009}}
27. ^{{cite journal |author1=Xiaokun Shu |author2=Antoine Royant |author3=Michael Z. Lin |author4=Todd A. Aguilera |author5=Varda Lev-Ram |author6=Paul A. Steinbach |author7=Roger Y. Tsien | title = Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome | journal = Science | volume = 324 | pages = 804–07 | year = 2009 | doi = 10.1126/science.1168683 | pmid = 19423828 | issue = 5928 | pmc = 2763207 }}
28. ^1 {{Cite journal|last=Rodriguez|first=Erik A.|last2=Tran|first2=Geraldine N.|last3=Gross|first3=Larry A.|last4=Crisp|first4=Jessica L.|last5=Shu|first5=Xiaokun|last6=Lin|first6=John Y.|last7=Tsien|first7=Roger Y.|date=2016-08-01|title=A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein|journal=Nature Methods|doi=10.1038/nmeth.3935|issn=1548-7105|pmid=27479328|volume=13|issue=9|pages=763–9|pmc=5007177}}
29. ^{{Cite journal|last=Tsien|first=Roger Y.|date=1998-01-01|title=The Green Fluorescent Protein|journal=Annual Review of Biochemistry|volume=67|issue=1|pages=509–544|doi=10.1146/annurev.biochem.67.1.509|pmid=9759496}}
30. ^{{Cite web | url=https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-indicators-for-zn2-and-other-metal-ions.html | title=Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7 - US}}
31. ^{{cite journal |vauthors=Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikurak M, Tsien RY | title = Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. | journal = Nature | volume = 388 | pages = 882–7 | year = 1997 | pmid = 9278050 | doi = 10.1038/42264 | issue = 6645 }}
32. ^{{cite journal |author1=B. Albert Griffin |author2=Stephen R. Adams |author3=Roger Y. Tsien | title = Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells | journal = Science | volume = 281 | pages = 269–72 | year = 1998 | doi = 10.1126/science.281.5374.269 | pmid = 9657724 | issue = 5374 }}
33. ^{{cite web|url=http://www.healthimaging.com/index.php?option=com_articles&view=portal&id=publication:10:article:21021:pnas-fluorescence-mr-imaging-probe-can-guide-cancer-surgery&division=hiit |title=PNAS: Fluorescence + MR imaging probe can guide cancer surgery |format=php |publisher= PNAS|date=March 4, 2010 |accessdate=March 11, 2010 }}
34. ^Interview, Roger Tsien. [https://www.rsb.org.uk/images/pdf/Biochemistry_supplement_final_proofs.pdf The light fantastic], pages 7-9 in The Biologist, Biochemistry Supplement. Vol 62 No 5 supplement. Oct/Nov 2015
35. ^San Diego Science Festival Lunch with a Laureate
36. ^{{cite web|url=http://news.sina.com.cn/c/2008-10-09/001116418702.shtml |script-title=zh:诺贝尔化学奖得主钱永健系吴越国王34世孙 |trans-title=Nobel Chemistry Prize winner Roger Tsien is the 34th-generational descendant of the King of Wuyue |publisher=Sina.com |date=October 9, 2008 |accessdate=October 9, 2008 |language=Chinese}}
37. ^{{cite journal |author=Ruth Williams |title=People & Ideas – Roger Tsien: Bringing color to cell biology |journal=J Cell Biol |volume=179 |issue=1 |pages=6–8 |date=October 8, 2007 |pmid=17923526 |pmc=2064723 |doi=10.1083/jcb.1791pi |last2=Horsfall |first2=MJ |last3=Van Helten |first3=JB |last4=Glickman |first4=BW |last5=Mohn |first5=GR}}
38. ^{{cite journal|last1=Ma|first1=Huan|last2=Li|first2=Boxing|last3=Tsien|first3=Richard W.|title=Distinct roles of multiple isoforms of CaMKII in signaling to the nucleus|journal=Biochimica et Biophysica Acta (BBA) - Molecular Cell Research|volume=1853|issue=9|year=2015|pages=1953–1957|issn=0167-4889|doi=10.1016/j.bbamcr.2015.02.008|pmid=25700840|pmc=4522395}}
39. ^Steele, D. (2004) Cells aglow. HHMI Bulletin, Summer 2004, 22–26
40. ^Huang, Christopher L.-H. (2018). "Roger Yonchien Tsien. 1 February 1952—24 August 2016". Biographical Memoirs of Fellows of the Royal Society. doi:[https://royalsocietypublishing.org/doi/10.1098/rsbm.2018.0013 10.1098/rsbm.2018.0013]
41. ^1 2 3 {{cite web |url=http://ucsdnews.ucsd.edu/pressrelease/nobel_laureate_roger_tsien_dies_age_64 |title=Nobel Laureate Roger Tsien Dies, Age 64 |last1=LaFee |first1=Scott |date=31 August 2016 |publisher=UC San Diego News Center |access-date=31 August 2016}}
42. ^{{cite web |url=http://www.sandiegouniontribune.com/news/2016/aug/31/roger-tsien-dies |title=UCSD Nobel laureate Roger Tsien dies |last1=Robbins |first1=Gary |date=31 August 2016 |publisher=The San Diego Union-Tribune, LLC |access-date=31 August 2016 |deadurl=yes |archiveurl=https://web.archive.org/web/20160901151811/http://www.sandiegouniontribune.com/news/2016/aug/31/roger-tsien-dies/ |archivedate=September 1, 2016 |df=mdy-all }}
43. ^{{Cite web | url=http://www.latimes.com/local/lanow/la-me-roger-tsien-20160831-snap-story.html | title=Roger Tsien, who was awarded the 2008 Nobel Prize in chemistry, dies at 64}}
44. ^{{cite newspaper|url=http://www.sandiegoreader.com/news/2016/sep/21/radar-death-and-un-taxing-salary/|title=Details scarce in death of Nobel Prize–winner|first=Matt|last=Potter|date=September 21, 2016|accessdate=May 6, 2017|newspaper=San Diego Reader}}
45. ^{{cite newspaper|url=http://www.sandiegoreader.com/news/2016/sep/21/radar-death-and-un-taxing-salary/|title=Nobel-Winning Chemist Created a Rainbow of Colors to Illuminate Cells|first=James R.|last=Hagerty|date=September 9, 2016|accessdate=May 6, 2017|newspaper=Wall Street Journal}}
46. ^{{cite web |url = http://www.mdc-berlin.de/en/news/2002/20021121-prof__roger_tsien_honoured_with_max_delbru/index.html |title = Prof. Roger Tsien Honoured with Max Delbrueck Medal |format= Web page |publisher=Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch |author=Barbara Bachtler |date=November 21, 2002 |accessdate=October 8, 2008}}
47. ^{{cite web |url=http://www.rose.brandeis.edu/Center/rose_past.html |title=Lewis S. Rosenstiel Award for Distinguished Work in Basic Medical Science Past Winners |publisher=Rosenstiel Basic Medical Sciences Research Center |year=2006 |accessdate=December 31, 2009 |deadurl=yes |archiveurl=https://web.archive.org/web/20100623224059/http://www.rose.brandeis.edu/Center/rose_past.html |archivedate=June 23, 2010 |df=mdy-all }}
48. ^{{cite web|url=http://royalsociety.org/page.asp?tip=1&id=4710 |title=New Foreign members & Honorary Fellow 2006 |format=Web page |publisher=The Royal Society |year=2006 |accessdate=September 27, 2008}}
49. ^{{cite web|url=http://www.rsc.org/Membership/AboutRscMembership/HonFRSC/index.asp |title=Who are our Honorary Fellows? |publisher=The Royal Society of Chemistry |format=asp |accessdate=December 27, 2009}}
50. ^{{cite web|url=http://www.chinapost.com.tw/taiwan/int%27l--community/2008/10/16/178879/New-Nobel.htm |title=New Nobel laureate to be named honorary academician |work=The China Post |location=Taiwan (ROC) |date=October 16, 2008 |accessdate=December 27, 2009}}
51. ^{{cite web|url=http://www.eweek.org/site/pdfs/2009_AAEOY_Highlights.pdf |title=2009 AAEOY Awards |publisher=National Engineers Week Foundation |author=Roger S. Dong |volume=XXIV |issue=4 |date=April 2009 |accessdate=December 27, 2009}}
52. ^{{cite web|url=http://invent.ucsd.edu/info/salute_may09.shtml |title=Salute to Innovation |format=shtml |publisher=UC San Diego Technology Transfer Office (TTO) |date=May 20, 2009 |accessdate=May 20, 2009}}
53. ^{{cite web |url = http://www.hku.hk/press/news_detail_6047.html |title = HKU to award Honorary Degree to Nobel Laureate Professor Roger Yonchien Tsien |publisher=The University of Hong Kong |date=Oct 29, 2009 |accessdate=October 29, 2009}}
54. ^{{cite web |url = http://www.cuhk.edu.hk/cpr/pressrelease/091210e.htm |title=The Chinese University of Hong Kong Holds 67th Congregation for Conferment of Degrees |publisher=The Chinese University of Hong Kong |date=December 10, 2009 |accessdate=December 10, 2009}}
55. ^{{cite web |url = http://www.indiaeducationdiary.in/Showlatest.asp?newsid=2341 |title=PM Dr. Manmohan Singh gives away awards to prominent scientists |format=asp |publisher=India Education Dairy.com |date=January 3, 2010 |accessdate=January 4, 2010}}
56. ^{{cite web |url = http://www.rsc.org/ScienceAndTechnology/Awards/SpiersMemorialAward/2010Winner.asp |title = RSC Spiers Memorial Award 2010 winner – Roger Tsien, UCSD, USA |format=asp |publisher=The Royal Society of Chemistry |year=2009 |accessdate=December 22, 2009}}
57. ^{{cite web |title=Green Fluorescent Protein |url = http://www.goldengooseaward.org/awardees/200352el6rpcnd3yibqw96m4yxtpvu |publisher=The Golden Goose Award |accessdate=2015-05-27 }}
58. ^{{cite web |url = http://www.the-aps.org/mm/awards/Other-APS-Awards/Recipients/Bowditch.html |title = Bowditch Award Lecture |accessdate = March 23, 2015 |publisher=American Physiological Society |website = Recipients }}
59. ^{{cite web|url=http://newsletter.sinica.edu.tw/news/read_news.php?nid=4288 |script-title=zh:本院邀請諾貝爾化學獎得主錢永健教授12月蒞臨「中央研究院講座」演講 |publisher=Academia Sinica |date=December 11, 2009 |accessdate=December 27, 2009 |language=zh}}
60. ^{{cite web|url=http://www.etaiwannews.com/etn/news_content.php?id=1129640&lang=eng_news&cate_img=logo_taiwan&cate_rss=TAIWAN_eng |title=Academia Sinica to welcome lectures by 2008 Nobel laureate |publisher=eTaiwan News (Central News Agency) |date=December 11, 2009|accessdate=December 27, 2009}}
61. ^{{cite web|url=http://www.biophysics.org/Program/ScientificSessions/NationalLecture/tabid/791/Default.aspx |title=The 2010 National Lecture will be given by Roger Tsien, UCSD |format=aspx |publisher=The Biophysical Society |year=2009 |accessdate=September 10, 2009}}
62. ^http://blogs.ucl.ac.uk/events/2011/10/26/prize-lecture-in-clinical-science-roger-tsien/

External links

  • {{Commons category-inline|Roger Tsien}}
{{University of California, San Diego}}{{Wolf Prize in Medicine}}{{Heineken Prizes}}{{Nobel Prize in Chemistry Laureates 2001-2025}}{{Ethnic Chinese Nobel laureates}}{{FRS 2006}}{{Authority control}}{{DEFAULTSORT:Tsien, Roger Y.}}

22 : 1952 births|2016 deaths|American biochemists|American Nobel laureates|American scientists of Chinese descent|Alumni of Churchill College, Cambridge|Han Chinese Nobel laureates|Fellows of the American Academy of Arts and Sciences|Foreign Members of the Royal Society|Harvard College alumni|Marshall Scholars|Members of the European Molecular Biology Organization|Members of the United States National Academy of Sciences|Nobel laureates in Chemistry|People from Livingston, New Jersey|Tsien family|University of California, Berkeley faculty|University of California, San Diego faculty|Wolf Prize in Medicine laureates|Winners of the Heineken Prize|Howard Hughes Medical Investigators|Descendants of Qian Liu
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