- Member clubs for the 2016–17 season
- Season summaries
{{Unreferenced|date=December 2009}}{{Infobox football league
| logo =
| pixels =
| country = {{SCO}}
| founded = {{Start date and age|2002|paren=yes}}
| folded = 2018
| teams = 12
| promotion = Super League First Division
| relegation = none
| level =
| domest_cup = Scottish Junior Cup
| champions = Dalry Thistle
| season = 2017–18
| current =
| website = SJFA
}}The Scottish Junior Football Ayrshire Division One (also known as the Ayrshire District League) was the third-tier division of the West Region of the Scottish Junior Football Association. The two highest-placed teams at the end of the season were promoted to the Super League First Division. Member clubs for the 2016–17 season| Club | Location | Home Ground | Finishing position 2015–16 |
|---|
| Annbank United | Annbank | New Pebble Park | 9th | | Ardeer Thistle | Stevenston | Ardeer Stadium | 11th | | Ardrossan Winton Rovers | Ardrossan | Winton Park | Super League First Division, 12th | | Craigmark Burntonians | Dalmellington | Station Park | 7th | | Dalry Thistle | Dalry | Merksworth Park | 8th | | Darvel | Darvel | Recreation Park | 3rd | | Kello Rovers | Kirkconnel | Nithside Park | 6th | | Lugar Boswell Thistle | Lugar | Rosebank Park | 5th | | Maybole | Maybole | Ladywell Stadium | Super League First Division, 14th | | Muirkirk | Muirkirk | Burnside Park | 10th | | Saltcoats Victoria | Saltcoats | Campbell Park | 12th | | Whitletts Victoria | Ayr | Dam Park | 4th |
Season summaries| Season | Champions | Also promoted |
|---|
| 2002–03 | Lugar Boswell Thistle | N/A | | 2003–04 | Maybole | Saltcoats Victoria | | 2004–05 | Largs Thistle | Annbank United | | 2005–06 | Irvine Meadow | Girvan | | 2006–07 | Kilbirnie Ladeside | Maybole | | 2007–08 | Hurlford United | Girvan | | 2008–09 | Dalry Thistle | Whitletts Victoria | | 2009–10 | Hurlford United | Kilwinning Rangers | | 2010–11 | Ardrossan Winton Rovers | Dalry Thistle | | 2011–12 | Maybole | Kello Rovers | | 2012–13 | Kilwinning Rangers | Troon | | 2013–14 | Irvine Victoria | Ardeer Thistle | | 2014–15 | Ardrossan Winton Rovers | Kilwinning Rangers | | 2015–16 | Girvan | Irvine Victoria | | 2016–17 | Darvel | Kello Rovers | | 2017–18 | Dalry Thistle | Irvine Victoria |
{{SJFA Ayrshire District League}}{{Scottish Junior Football Association}}{{Football in Scotland}}{{Scotland-footy-competition-stub}} 1 : Scottish Junior Football Association, West Region |