“Sequencing by hybridization”的意思、由来-开放百科全书

The binding of one strand of DNA to its complementary strand in the DNA double-helix (known as hybridization) is sensitive to even single-base mismatches when the hybrid region is short or if specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notably via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations.^[2]^[3]

The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation (as well as pore-based methods). However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, and reversible hybridization.^[4]

Examples of commercial systems

See also

References

1. ^{{cite journal|last1=Drmanac |first1=R |display-authors=et al.|title=Sequencing by hybridization (SBH): advantages, achievements, and opportunities.|journal=Adv Biochem Eng Biotechnol|date=2002|volume=77|pages=75–101|pmid=12227738}}
2. ^{{cite journal|last1=Preparata |first1=FP|last2=Upfal |first2=E |title=Sequencing-by-hybridization at the information-theory bound: an optimal algorithm|journal=J. Comput. Biol.|date=2000|volume=7|issue=3|pages=621–30|pmid=11108482 |doi=10.1089/106652700750050970|citeseerx=10.1.1.61.3325}}
3. ^{{cite journal|last1=Hanna |first1=GJ |display-authors=et al|title=Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase|journal= J Clin Microbiol|date=July 2000|volume=38|issue=7|pages=2715–2721|pmid=10878069|pmc=87006}}
4. ^{{cite journal|last1=Church|first1=George M. |title=Genomes for all.|journal=Scientific American|date=January 2006|volume=294|issue=1|pages=46–54 |doi=10.1038/scientificamerican0106-46|pmid=16468433}}